Cryostats cm1950

Manufactured by Leica

The Leica Cryostats CM1950 is a precision instrument designed for cryosectioning of frozen tissue samples. It provides accurate temperature control and rapid cooling capabilities to enable high-quality sectioning of frozen specimens for various analysis and research applications.

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Lab products found in correlation

Anti collagen 1, by Abcam (1 mentions) Anti pcna, by Santa Cruz Biotechnology (1 mentions) Anti gfap, by Abcam (1 mentions) Anti serotonin, by Merck Group (1 mentions) Anti caspase 3, by BD (1 mentions) Anti laminin, by Merck Group (1 mentions) Anti α sma, by Abcam (1 mentions) P6748, by Merck Group (1 mentions) Anti rabbit or anti mouse secondary antibody, by Thermo Fisher Scientific (1 mentions) Glial fibrillary acidic protein (gfap), by Abcam (1 mentions) P6148, by Merck Group (1 mentions) Bacto agar, by Merck Group (1 mentions) Ab23730, by Abcam (1 mentions) S7903, by Merck Group (1 mentions)

Spelling variants of this product

Cryostat (2434 citations) CM1950 (1130 citations) CM1950 cryostat (399 citations) CM1950 cryostat microtome (17 citations) Leica CM1950 Clinical Cryostat (7 citations) CM1950‐S cryostat (2 citations)

3 protocols using cryostats cm1950

Microscopic Imaging of Anammox Biofilm

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Microscopic imaging was conducted with a Leica SPP8WLL confocal microscope using a 40× objective. Briefly, cryosectioned anammox biofilm (Leica Cryostats CM1950) (5 μm) gel formed during dialysis and purified protein were stained with 0.5% (wt/vol) ThT, each prepared using 0.1 N filtered HCl, for 15 min before being viewed under the microscope. ThT was excited at 405 nm and detected at 488 to 520 nm.

Seviour T., Wong L.L., Lu Y., Mugunthan S., Yang Q., Shankari U d/o Chanda S.e.g.a.r.a.n., Bessarab I., Liebl D., Williams R.B., Law Y, & Kjelleberg S. (2020). Phase Transitions by an Abundant Protein in the Anammox Extracellular Matrix Mediate Cell-to-Cell Aggregation and Biofilm Formation. mBio, 11(5), e02052-20.

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Zebrafish Immunofluorescence Staining Protocol

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Zebrafish larvae were fixed in 4% paraformaldehyde in phosphate buffered saline (Sigma, P6748) and cryo-sectioned at 8 µm thickness using a Leica Cryostats CM1950, followed by immunofluorescence (IF) staining. The primary antibody was derived either from rabbits (anti-PCNA, Santa Cruz, FL-261; anti-Caspase-3, BD biosciences, C92-065; anti-Serotonin, Sigma, C5545; anti-Tgfb1, Anaspec, 55450; anti-Collagen I, Abcam, ab23730; anti-laminin, Sigma, L9393; anti-α-Sma, Abcam, ab15734) or from mice (Anti-Gfap, Abcam, 154474). Anti-mouse or anti-rabbit secondary antibodies were purchased from Thermo Fisher Scientific.

Yang Q., Yan C, & Gong Z. (2018). Interaction of hepatic stellate cells with neutrophils and macrophages in the liver following oncogenic kras activation in transgenic zebrafish. Scientific Reports, 8, 8495.

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Immunofluorescence Profiling of Larval Fish

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All of the fish larvae were fixed in 4% paraformaldehyde (P6148; Sigma) in phosphate buffered saline, embedded in 1.5% Bacto-agar (A5306; Sigma), immersed in 30% sucrose (S7903; Sigma) in phosphate buffered saline and cryo-sectioned at 8 μm thickness using a Leica Cryostats CM1950, followed by immunofluorescence (IF) staining. The primary antibodies used included anti-proliferating cell nuclear antigen (PCNA) (FL-261; Santa Cruz Biotechnology, Dallas, TX), anti–Caspase 3 (C92-065; BD Biosciences, Singapore), anti–collagen I (ab23730; Abcam), anti-laminin (L9393; Sigma), anti-glial fibrillary acidic protein (Gfap) (154474; Abcam), anti-a-Sma (ab15734; Abcam, Singapore), anti-fibrinogen (sc69775; Santa Cruze) and anti-Tgfb1 (04-953; EMD Millipore, Billerica, MA). Anti-rabbit or anti-mouse secondary antibodies were purchased from Thermo Fisher Scientific. At least 10 fish larvae from each group were examined and at least one image was taken for each larva.

Yan C., Yang Q, & Gong Z. (2018). Activation of Hepatic Stellate Cells During Liver Carcinogenesis Requires Fibrinogen/Integrin αvβ5 in Zebrafish. Neoplasia (New York, N.Y.), 20(5), 533-542.

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