For blood glucose measurement, blood samples were collected into heparinized glass capillary tubes and immediately suspended in glucose/lactate system solution buffer (EKF-Diagnostics, Barleben, Germany). Blood glucose was measured using a BIOSEN c-Line glucose meter (EKF-Diagnostics), according to the manufacturer's instructions. For insulin measurement, blood samples were similarly collected in heparinized tubes and plasma was separated and stored at −80°C until analysis. Mouse insulin was measured using the Meso Scale Diagnostics platform.
Biosen c line glucose meter
Manufactured by EKF Diagnostics
Sourced in Germany
The BIOSEN c-Line glucose meter is a compact, easy-to-use device designed for measuring glucose levels in various biological samples, such as blood, plasma, or serum. It provides accurate and reliable glucose measurements with a user-friendly interface.
Automatically generated - may contain errors
Lab products found in correlation
Glucose lactate system solution buffer, by EKF Diagnostics (3 mentions)
Meso scale diagnostics platform, by Mesoscale (2 mentions)
Mouse albumin elisa kit, by Fortis Life Sciences (2 mentions)
Crep2 kit, by Roche (2 mentions)
Cobas c 501 autoanalyzer, by Roche (2 mentions)
Cobas c111, by Roche (1 mentions)
Ultrasensitive insulin elisa, by Mercodia (1 mentions)
Glucose, by Fresenius (1 mentions)
Ascensia breeze 2 blood glucose monitoring system, by Bayer (1 mentions)
Alphalisa kit, by PerkinElmer (1 mentions)
8 protocols using biosen c line glucose meter
1
Blood Glucose and Insulin Measurement
2
Blood Glucose Measurement Protocol
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10 μL blood was collected in a heparinized glass capillary and immediately suspended in 0.5 ml lysis buffer (glucose/lactate system solution, EKF-diagnostics, Cardiff, Great Britain), and then analyzed for glucose using a BIOSEN c-Line glucose meter (EKF-diagnostics).
3
Glycemic and Renal Biomarkers Monitoring
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Blood glucose was measured weekly for the first 4 weeks postsurgery and from there on every third week by collecting blood into heparinized glass capillary tubes and immediate suspension in glucose/lactate system solution buffer (EKF‐diagnostics). Blood glucose was measured using a BIOSEN c‐Line glucose meter (EKF‐diagnostics) according to the manufacturer's instructions. Glycated haemoglobin A1c (HbA1c) levels were measured at termination (16 weeks after surgery) using commercial kits (Roche Diagnostics) on a CobasTM C‐501 autoanalyzer (Roche Diagnostics). At study week 15, spot urine samples were collected directly from the penis/vulva to determine the urinary albumin‐to‐creatinine ratio (ACR). Urine creatinine was measured using the CREP2 kit (Roche Diagnostics) on a CobasTM C‐501 autoanalyzer. Urinary albumin was measured using a mouse albumin ELISA kit (Bethyl Laboratories, Inc.). Plasma blood urea nitrogen (BUN) levels were measured at termination (16 weeks after surgery) using commercial kits (Roche Diagnostics) on the Cobas™ C‐501 autoanalyzer.
4
Measuring Blood Glucose and Urine ACR in Mice
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Blood glucose was measured throughout the study by collecting blood from the tail vein of nonfasted mice into heparinized glass capillary tubes and immediately suspending in glucose/lactate system solution buffer (EKF Diagnostics, Barleben, Germany). Blood glucose was measured using a BIOSEN c-Line glucose meter (EKF Diagnostics) according to the manufacturer's instructions. Spot urine samples were collected 6 and 12 weeks after the injection of viral vector directly from the vulva to determine the urine ACR. Urine creatinine was measured using the CREP2 kit (Roche Diagnostics, Mannheim, Germany) on a Cobas C-501 autoanalyzer. Urine albumin was measured using a Mouse Albumin ELISA Kit (Bethyl Laboratories, Montgomery, TX, USA).
5
Evaluating SER140 Efficacy on Nonfasting Glucose
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Nonfasting blood glucose (BG) was monitored biweekly before the experiment start. On day 3, animals were randomized according to BG and then body weight into two groups: a vehicle group (QD) (n = 20) and a SER140 group, 10 mg/kg (QD) (n = 20). The compound was administered subcutaneously once daily. SER140 was provided by Phlogo ApS, Copenhagen, Denmark (5 mg/mL in water), and diluted in PBS at the required concentration for injection (10 mg/kg, S.C). Throughout the study, animals had ad libitum access to food and water. Body weight and food and water intake were recorded biweekly from arrival and throughout the study period. Samples for measuring nonfasting BG were collected biweekly from the tail vein. Animals were terminated on day 56 and BG was measured using a BIOSEN c-Line glucose meter (EKF Diagnostics, Germany), HbA1c using autoanalyzer Cobas C-111 with commercial kit (Roche Diagnostics, Germany), and insulin using ultrasensitive insulin ELISA (Mercodia, Sweden) according to the manufacturer's instructions.
6
Oral Glucose Tolerance Test in Animals
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An oral glucose tolerance test (OGTT) was performed on treatment day 27. Animals were fasted 4 h prior to the OGTT. Vehicle or compounds were administered 60 min prior to the OGTT. At time 0, a bolus of glucose (2 g/kg, 10 ml/kg, Fresenius Kabi, Uppsala, Sweden) was administered by oral gavage. Tail vein blood samples were collected in 10 µl heparinized capillary tubes at times −60, 0, 15, 30, 60, 120 and 240 min., and immediately suspended in glucose/lactate solution buffer (0.5 mL, EKF-diagnostics, Cardiff, UK). Blood glucose concentrations were measured using a BIOSEN C-Line glucose meter (EKF-diagnostics, Barleben, Germany) according to the manufacturer’s instructions.
7
Blood Glucose and Insulin Measurement
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Blood was sampled from a tail vein or tail snips in mice and a peripheral vein in monkeys. Blood glucose from tail snips was determined at the specified time points using the Ascensia Breeze 2 blood glucose monitoring system (Bayer Healthcare LLC, Mishawaka, IN, USA) in the acute mouse studies. Blood glucose from venous samples was measured with a BIOSEN c‐Line glucose meter (EKF‐diagnostics, Barleben, Germany) in the repeat dose DIO mouse study or with an ACCU‐CHEK glucose meter (La Roche AG, Basel, Switzerland) in monkeys. Blood was collected into tubes containing EDTA anticoagulant, centrifuged at 2000 × g for 10 minutes at ambient temperature and plasma immediately frozen prior to measurement of insulin by immunoassay using a Mesoscale rat/mouse insulin kit (K152BZC‐1, Rockville, MD, USA) in the mouse acute IPGTT study, an AlphaLisa kit (Perkin Elmer, Waltham, MA, USA) in the repeat dose DIO mouse study and an EMD Millipore kit (Nottingham, UK) in monkeys. Access to food was withheld for monkeys for at least 3 hours prior to sampling for glucose and insulin.
8
Metabolic Biomarker Measurement Protocol
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Fasting blood glucose was measured using a BIOSEN c-Line glucose meter (EKF-Diagnostics), insulin was measured using the Meso Scale Diagnostics platform, TG and TC were measured using commercial kits (Roche Diagnostics) on the Cobas C-501 autoanalyzer (Roche), and FFA levels were measured using a Wako Chemicals kit on the Cobas C-501 autoanalyzer, all according to the manufacturers’ instructions. Homeostatic model assessment of insulin resistance (HOMA-IR) was calculated using the following equation: [fasting serum glucose (mmol/L) × fasting serum insulin (pmol/L) / 22.5] to assess insulin resistance [57 (link)].
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