Between 10 and 20 male pupae derived from each combination were immobilized by freezing (4 °C) for 5 min. The length of the cephalothorax was measured to determine pupal size [34 (link)]. The pupae were then preserved until the adult eclosion for wing measurement. The right wing (or left if the right was damaged or lost) was separated from the body under a dissecting microscope. A wing was measured from the distal edge of the alula to the end of the radius vein excluding fringe scales [35 (link)]. A digital image of the wing was captured using a camera (EI200 HD, OPTI Advanced Imaging Ltd., Taipei City, Taiwan) mounted on a stereomicroscope (Leica S8 AP0, Leica Microsystems, Heerbrugg, Switzerland). The image was analyzed using Helicon Focus version 6.7.1 Lite (Helicon Soft Ltd., Kharkiv, Ukraine).
Leica s8ap0
Manufactured by Leica Microsystems
Sourced in Germany
The Leica S8AP0 is a stereomicroscope designed for a variety of applications in the laboratory. It features an optical system with a magnification range of 6.3x to 50x, allowing for detailed observation and analysis of samples. The microscope is equipped with LED illumination for bright, even lighting.
Automatically generated - may contain errors
8 protocols using leica s8ap0
1
Measuring Pupal Size and Wing Dimensions in Males
2
Thelytokous Trichogramma Reproduction Control
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Thelytokous T. pretiosum were treated with either antibiotics curing (5.0 mg/mL sulfadiazine, 10.0 mg/mL tetracycline hydrochloride) or high-temperature treatment (30 ± 1 °C) [4 (link)]. Both antibiotics were mixed with 30% honey. T. pretiosum reared at a temperature of 25 ± 1 °C was used as the control. Every newly emerged female was allowed to oviposit for 24 h. The number of parasitic eggs, emergence rate, and male percentage of offspring were recorded for 5 generations by checking under a dissecting microscope Leica S8AP0 (Leica Microsystems, Nussloch GmbH, Wetzlar, Germany). Ten replicates for each treatment and control were detected.
3
Lichen Taxonomy and Identification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The field excursion was organized by Dr. X. Y. Wang, Kunming Institute of Botany, CAS in December 2013. Duplicates of the specimens were deposited in the herbarium of the Korean Lichen Research Institute (KoLRI), Sunchon National University, South Korea. The taxonomy of the material was studied in the lichenology laboratory of the CSIR-National Botanical Research Institute, Lucknow, India, using standard microscopy techniques. Morphological and anatomical details were observed under stereozoom (LEICA S8AP0; Leica Microsystems, Chennai, India) and compound (LEICA DM500; Leica Microsystems) microscopes, respectively. Relevant literature [2 15 16 17 18 19 20 ] was consulted for the identification of species. The color test and thin layer chromatography in solvent systems A [toluene (180) : 1, 4-dioxane (45) : acetic acid (5)] and C [toluene (170) : acetic acid (30)] were performed following Orange et al. [21 ]. Lugol's solution (I) was used to check the amyloidity of asci and ascospores. Illustrations were prepared using CorelDRAW (version 12).
4
Sciatic Nerve and Muscle Histology
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Twelve weeks after surgery, the rats were deeply anesthetized with an intraperitoneal (IP) injection (300 mL) of a cocktail containing xylasin (3.2 mg/kg; Syntec, Cotia, SP, Brazil), ketamine (62.5 mg/kg; Syntec), and acepromazine (0.625 mg/kg; Syntec) diluted in water and perfused transcardially with a 0.9% saline solution for 10 min (10 mL/min, Masterflex®; Cole-Parmer Instrument Co.), followed by 4% paraformaldehyde (20 min) and 4% paraformaldehyde + 10% sucrose solution (10 min). The sciatic nerves or gastrocnemius muscles were kept in a 30% sucrose solution in phosphate buffer for 48 h. The nerves had the epineurium removed and were photographed under a stereomicroscope (Leica S8AP0; Leica Microsystems Nussloch GmbH). After embedding in OCT mounting medium, longitudinal sections of the sciatic nerves (18 μm) or gastrocnemius muscles (30 μm) were obtained using a cryostat (Leica CM 1850‘ Leica Microsystems Nussloch GmbH) and mounted directly on gelatin + 0.05% chromium alum precoated slides. Slides were stored at −20 °C for immunofluorescence procedures.
5
SMALDI Mass Spectrometry Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Areas of interest of the PDA with the seeded or cultivated microorganisms were cut to rectangular pieces with a razor blade and transferred onto a glass slide and fixed with thin 25 µm adhesive tape. A SMALDI Prep spray device (TransMIT GmbH, Germany) was utilized for matrix deposition and formation of uniform matrix layer on the samples. HCCA (alpha-cyano-4-hydroxycinnamic acid, 7 mg/mL) in acetone:water 1:1 (v:v) containing 0.1% FA was used as matrix solution and the spray application was performed using the following parameters: 20 µL/min matrix flow rate, 3 L/min gas (N2) flow rate, 30 min spray cycle, and 100 rpm sample plate revolution speed. Photos of each sample were created before spraying with the optical microscope Leica S8AP0 (Leica Microsystems GmbH, Germany) or the digital microscope VHX-5000 (Keyence Deutschland GMBH, Germany), respectively.
6
Preparing Camponotus rufipes Ant Workers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Workers were obtained from a mature colony of Camponotus rufipes. The colony was collected in 2011 from La Coronilla, Uruguay, by Oliver Geißler and kindly provided by F. Roces (University of Würzburg). The colony was kept at 25°C and 50% rH at the University of Konstanz with a 12 h:12 h L/D-cycle. Honeywater and cockroaches or locusts were provided twice a week. Workers were collected from the colony and immobilized on a glasslide with adhesive tape. The mandibles and the scape were mounted in dental wax (Surgident, Heraeus Kulzer, Germany). The flagellum of the antenna was mounted under visual control (Leica S8AP0, Leica, Microsystems, Wetzlar, Germany) with water based white-out correction fluid (Tipp-Ex, Bic, France) exposing the lateral-ventral side upwards.
7
Measuring Mosquito Wing Length
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Newly emerged female mosquitoes were transferred to mosquito cages (measuring 32.5 cm in length, 32.5 cm in width, and 32.5 cm in height) and provided with 10% sucrose solution. Subsequently, 30 5-day-old female mosquitoes from each population were randomly collected and stored at −20°C for wing length measurement. A pair of wings from each female was mounted on a microscope slide (Leica S8 AP0, Leica Microsystems, Heerbrugg, Switzerland). A digital image of the wings was captured using a camera (EI200 HD, OPTI Advanced Imaging Ltd., Taipei City, Taiwan), and the distance between veins R3 and R4+5 was measured from the axillary incision (or alula notch) to the apex of the wing, excluding the fringe scales [37 (link)]. The images were analyzed using Helicon Focus Lite version 6.7.1 (Helicon Soft Ltd., Kharkiv, Ukraine).
8
Induced Immune Response in N. lugens
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
N. lugens 5th instar nymphs were immunized with a microinjection of heat-killed Escherichia coli K12 or Bacillus subtilis (500 cells per nymph, respectively) using the FemtoJet Microinjection System (Eppendorf-Nethler-Hinz, Hamburg, Germany). Nymphs were collected at different time points (6, 12 and 24 hours) after infection for the bacteria-induced expression experiment as previously described
[13 (link)].
For studies of tissue-specific expression, the N. lugens were dissected under a Leica S8AP0 stereomicroscope (Leica Microsystems GmbH, Wetzlar, Germany). The fat body, midgut, salivary gland, male reproductive system, ovary and carcass (the remaining body) were isolated and quickly washed in cold diethylpyrocarbonate (DEPC)-treated NaCl/Pi solution (137 mM NaCl, 2.68 mM KCl, 8.1 mM Na2HPO4, 1.47 mM KH2PO4, pH 7.4) and immediately frozen at -80°C as previously described
[2 (link), 13 (link)]. For each tissue, more than 300 N. lugens individuals were dissected and used for RNA extractions.
[13 (link)].
For studies of tissue-specific expression, the N. lugens were dissected under a Leica S8AP0 stereomicroscope (Leica Microsystems GmbH, Wetzlar, Germany). The fat body, midgut, salivary gland, male reproductive system, ovary and carcass (the remaining body) were isolated and quickly washed in cold diethylpyrocarbonate (DEPC)-treated NaCl/Pi solution (137 mM NaCl, 2.68 mM KCl, 8.1 mM Na2HPO4, 1.47 mM KH2PO4, pH 7.4) and immediately frozen at -80°C as previously described
[2 (link), 13 (link)]. For each tissue, more than 300 N. lugens individuals were dissected and used for RNA extractions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!