Triglyceride (TG) contents were measured using the triglyceride colorimetric assay kit (Abcam, MA, USA) according to the manufacturer’s protocol. Briefly, frozen worm pellets were placed in liquid nitrogen with 5% Triton X-100. The pellets were sonicated and diluted for protein determination by BCA assay (Pierce, IL, USA). The samples were heated till 80 °C and shaken for 5 min then, samples were cool-down to room temperature to solubilize all the triglycerides. TG contents were normalized relative to protein contents and three independently collected worm pellets were assayed for each experimental condition.
Triglyceride colorimetric assay kit
Manufactured by Abcam
Sourced in United States
The Triglyceride Colorimetric Assay Kit is a laboratory product that allows for the quantitative determination of triglyceride levels in various sample types. It utilizes a colorimetric reaction to measure the concentration of triglycerides present in the sample.
Automatically generated - may contain errors
Lab products found in correlation
Cell counting kit 8 cck 8, by Merck Group (1 mentions)
Protease inhibitor, by Merck Group (1 mentions)
X tremegene sirna transfection reagent, by Merck Group (1 mentions)
3t3 l1 differentiation kit, by Abcam (1 mentions)
Nile red staining kit, by Abcam (1 mentions)
Lipid oil red o staining kit, by Abcam (1 mentions)
Mouse 3t3 l1 preadipocytes cl 173, by American Type Culture Collection (1 mentions)
Microtiter plate reader, by Agilent Technologies (1 mentions)
Synergy ht multi mode microplate reader, by Agilent Technologies (1 mentions)
5 protocols using triglyceride colorimetric assay kit
1
Triglyceride Quantification in Worms
2
3T3-L1 Preadipocyte Differentiation and Analysis
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The following reagents were obtained from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany): HAuCl4·3H2O, ethanol, Cell Counting Kit-8 (CCK-8), protease inhibitor, DAPI mounting media, and X-treme GENE siRNA transfection reagent. Mouse 3T3-L1 preadipocytes (CL-173, Lot:70047755) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). The 3T3-L1 differentiation kit and lipid (Oil Red O) staining kit were obtained from BioVision (Milpitas, CA, USA). The Nile Red staining kit and triglyceride colorimetric assay kit were obtained from Abcam (Cambridge, MA, USA). The following reagents were obtained from Thermo Fisher Scientific Life Sciences (Waltham, MA, USA): Dulbecco’s modified Eagle’s medium/nutrient mixture F-12 (DMEM/F12), fetal bovine serum (FBS), phosphate-buffered saline (PBS), penicillin and streptomycin, eight-well chamber slides, M-PER™ Mammalian Protein Extraction Reagent, Pierce ECL Western blotting substrate, PureLink RNA Mini Kit, high-dose cDNA reverse kit, SYBR Green qPCR Master Mix, PLD1-specific siRNA, and negative control siRNA.
3
Quantification of Hepatic Triglycerides
4
Serum, Tissue Triglyceride and FFA Assay
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Serum and tissue triglycerides (TG) assay (Cayman Chemical, Triglyceride Colorimetric Assay Kit), free fatty acids (FFA) assay (FFA Kit, Biovision), and insulin assay (Insulin Kit, Cayman Chemical) were performed according to the instructions provided by the manufacture. For the hepatic TG, liver tissues of each rat were homogenized in NP-40 lysis buffer and centrifuged at 12,000 g for 30 min at 4°C.
5
Quantification of Hepatic Triglycerides
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Approximately 50–100 mg of frozen liver tissues (stored at − 80 °C) was mechanically disrupted and homogenized in lysis buffer containing 5% Triton X-100 by using a tissue homogenizer. The remained insoluble cellular fragments were then removed by centrifugation at 16,000×g for 10 min. Extracted triglycerides were quantified using a Triglyceride Colorimetric Assay Kit (Biovision, Inc.) according to the manufacturer’s protocol. Briefly, the sample supernatant was combined with a triglyceride probe, enzyme mix, and lipase, and after 60 min, incubation in the dark absorbance was measured at 570 nm in a Synergy HT Multi-Mode Microplate Reader (BioTek Instruments, Inc.). Blank and lipase controls were subtracted from the optical density of each sample to allow for the quantification of triglycerides. Triglyceride concentrations were interpolated from the linear regression of a standard curve and normalized by the wet weight of liver tissue used in the assay.
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