H 7000 electron microscope

Manufactured by Hitachi

Sourced in Japan, United States

The H-7000 is an electron microscope manufactured by Hitachi. It is designed to produce high-resolution images by using a focused beam of electrons to scan the surface of a specimen. The H-7000 provides detailed information about the structure and composition of materials at the nanoscale level.

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Lab products found in correlation

Ultracut e, by Leica (3 mentions) High resolution 4 k 4 k digital camera, by Ametek (2 mentions) Micrograph software, by Ametek (2 mentions) Nano z, by Malvern Panalytical (2 mentions) Q550cw, by Leica (1 mentions) Zetasizer nano z, by Malvern Panalytical (1 mentions) H 500, by Hitachi (1 mentions) Type a, by Ted Pella (1 mentions) Axiocam mr, by Zeiss (1 mentions) Remel flagella stain, by Thermo Fisher Scientific (1 mentions) Axio imager z1, by Zeiss (1 mentions) Quetol 812, by Nisshin EM (1 mentions) Tecnai transmission electron microscope, by Thermo Fisher Scientific (1 mentions) Epon 812, by TAAB (1 mentions)

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H-7000 (120 citations) H-7000 transmission electron microscope (30 citations) Hitachi-7000 electron microscope (5 citations) H-7000 microscope (3 citations) H-7000 NAR transmission electron microscope (2 citations)

38 protocols using h 7000 electron microscope

Negative Staining for Electron Microscopy

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The samples were deposited on carbon‐coated 300‐mesh copper grids, incubated for 3 min for absorption, and then washed by water. Negative staining was carried out by staining with 2% uranyl acetate for 1.5 min. After air drying, the samples were viewed using a Hitachi H‐7000 electron microscope (Hitachi, Japan).

Cheng Y., Chen Z., Liao T., Lin C., Shen H.C., Wang Y., Chang C., Liu R., Chen R.P, & Tu P. (2017). An intranasally delivered peptide drug ameliorates cognitive decline in Alzheimer transgenic mice. EMBO Molecular Medicine, 9(5), 703-715.

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Submandibular Gland Morphometric Analysis

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Submandibular gland tissues were fixed in 10% formalin, paraffin-embedded, sectioned at 5 μm, and stained with Hematoxylin-Eosin for morphometric evaluation using the Leica Qwin software (Q550CW, Leica, Mannheim, Germany). Other gland specimens were fixed in 2% paraformaldehyde-1.25% glutaraldehyde. Ultrathin sections were prepared and stained with uranyl acetate and lead citrate and observed by TEM (H-7000 electron microscope; Hitachi, Tokyo, Japan). The area of acinar cells, number of secretory granules, and area of secretory granules were calculated using the imaging software (Q550CW, Leica, Mannheim, Germany) and ImageJ (NIH, Bethesda, MD, U.S.A.). The data were from 9 randomly selected acini in each section from 12 control and 12 transplanted glands.

Ding C., Cong X., Zhang Y., Li S.L., Wu L.L, & Yu G.Y. (2018). β-adrenoceptor activation increased VAMP-2 and syntaxin-4 in secretory granules are involved in protein secretion of submandibular gland through the PKA/F-actin pathway. Bioscience Reports, 38(1), BSR20171142.

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Ultrastructural Analysis of Liver Tissue

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Con A-treated animals were sacrificed after 6 h of ConA administration and hepatic tissue were obtained as described previously [48 (link)]. Ultrathin sections (50 nm) were made and observed under a Hitachi H-7000 electron microscope (Hitachi, Tokyo, Japan).

Peixoto E., Atorrasagasti C., Malvicini M., Fiore E., Rodriguez M., Garcia M., Finocchieto P., Poderoso J.J., Corrales F, & Mazzolini G. (2016). SPARC gene deletion protects against toxic liver injury and is associated to an enhanced proliferative capacity and reduced oxidative stress response. Oncotarget, 10(41), 4169-4179.

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Hepatic Tissue Ultrastructural Analysis

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After 6 h, Con A-treated animals were killed and hepatic tissue dissected out and processed as described elsewhere.^{30 (link)} Ultrathin sections (50 nm) were made and observed under a Hitachi H-7000 electron microscope (Hitachi, Tokyo, Japan).

Peixoto E., Atorrasagasti C., Aquino J., Militello R., Bayo J., Fiore E., Piccioni F., Salvatierra E., Alaniz L., García M., Bataller R., Corrales F., Gidekel M., Podhajcer O., Colombo M, & Mazzolini G. (2014). SPARC (secreted protein acidic and rich in cysteine) knockdown protects mice from acute liver injury by reducing vascular endothelial cell damage. Gene therapy, 22(1), 9-19.

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Extraction and Purification of Viral Particles

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Thirty worker bees were frozen in liquid nitrogen, ground to a fine powder, and homogenized in 10-ml extraction buffer (0.1 M potassium phosphate buffer [pH 7.5], 0.2% diethyldithiocarbamate, 1/5 volume of diethyl ether). The mixture was emulsified with 5 ml carbon tetrachloride and centrifuged at 5,000 × g at 4°C for 30 min to remove tissue debris. Supernatant containing viruses was centrifuged once more at 5,000 × g at 4°C for 30 min and then filtered through a 45-μm filter to remove small tissue debris. The filtrate was then centrifuged at 10,187 × g for 6 h at 4°C to pellet the viral particles. The pellet was resuspended in 2 ml of 0.2 M phosphate-buffered saline (PBS) buffer. A 15-µl portion of viral solution was examined for the presence of virus particles in an electron microscope. The rest of the viral solution was saved for subsequent viral RNA isolation and cDNA library construction.
Virus particles were negatively stained with 2% uranyl acetate on a Formvar-coated Ni grid and viewed in a Hitachi H-7000 electron microscope at magnifications between ×33,000 and ×100,000.

Li J.L., Cornman R.S., Evans J.D., Pettis J.S., Zhao Y., Murphy C., Peng W.J., Wu J., Hamilton M., Boncristiani HF J.r., Zhou L., Hammond J, & Chen Y.P. (2014). Systemic Spread and Propagation of a Plant-Pathogenic Virus in European Honeybees, Apis mellifera. mBio, 5(1), e00898-13.

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TEM Examination of Biological Samples

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For TEM examination, a 10 μl sample was loaded onto a carbon-coated copper grid and dried on filter paper after 2 min. Then, the sample was negatively stained. After that, the samples were observed with a Hitachi H7000 electron microscope at 200 kV.

Chen C., Zou Z., Chen L., Ji X, & He Z. (2016). Functionalized magnetic microparticle-based colorimetric platform for influenza A virus detection. Nanotechnology, 27(43), 435102.

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Electron Microscopy of Glutaraldehyde-Fixed Cells

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TetPtf1a cells grown on p60 culture dishes to EB7+28 were fixed for 45 minutes in 3% glutaraldehyde, 1% paraformaldehyde, 0.1 M cacodylate buffer solution. Fixed tissues were then prepared and sectioned as previously described [18 (link)]. Sections were then examined using a Hitachi H-7000 electron microscope.

Nair G., Vincent R.K, & Odorico J.S. (2014). Ectopic Ptf1a expression in murine ESCs potentiates endocrine differentiation and models pancreas development in vitro. Stem cells (Dayton, Ohio), 32(5), 1195-1207.

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Ultrastructural Analysis of Mouse Placenta

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After perfusion with 4% PFA, some samples of the mouse placenta with uterus were cut into small blocks and incubated in 2% glutaraldehyde in 0.1 M PB for 2 hours. The samples were subsequently incubated with a 1% solution of OsO₄ for 1 hour at 4°C, dehydrated by passage through a graded series of ethanol followed by propylene oxide, and embedded in epoxy resin. Ultrathin sections (70 nm) were stained with lead citrate and examined with an H-7000 electron microscope (Hitachi, Tokyo, Japan).

Suenaga K., Kitahara S., Suzuki Y., Kobayashi M., Horie S., Sugawara J., Yaegashi N, & Sato Y. (2014). Role of the Vasohibin Family in the Regulation of Fetoplacental Vascularization and Syncytiotrophoblast Formation. PLoS ONE, 9(9), e104728.

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Ultrastructural Analysis of Cardiac Tissue

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Small cubic pieces ≤ 1 mm³ were dissected from left ventricles and fixed with 2.5% glutaraldehyde in 0.1 mol/ L sodium phosphate (pH 7.4) overnight at 4 °C. After post-fixation in 1% OsO4, samples were dehydrated through graded alcohols and embedded in Epon Araldite. Ultrathin sections (50 nm) were cut using an ultramicrotome (Ultracut E, Leica), and stained with uranyl acetate and lead citrate. The specimens were viewed on a Hitachi H-7000 Electron Microscope (Pleasanton, CA, USA). Images were captured using a Gatan high resolution 4 k × 4 k digital camera and Gatan Ditital Micrograph software (22 (link)).

Xu X, & Ren J. (2014). Macrophage Migration Inhibitory Factor (MIF) Knockout Preserves Cardiac Homeostasis through Alleviating Akt-Mediated Myocardial Autophagy Suppression in High Fat Diet-Induced Obesity. International journal of obesity (2005), 39(3), 387-396.

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Ultrastructural Analysis of RGM1 Cells

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RGM1 cells were treated with IND for 1 h after pretreatment with 5-µg/ml Qing Dai for 1 h. The cultures were fixed with 2% glutaraldehyde in 0.1 M phosphate buffer, pH 7.4. Thereafter, the cells were postfixed for 2 h in cold-buffered, 1% OsO₄ after experimental manipulations and were dehydrated in a graded series of ethanol and propylene oxide, and were embedded in Epon. Epon sections were cut on the Reichert-Jung ultramicrotome, were stained with uranyl acetate and lead citrate, and were then imaged under a Hitachi (H-7000) electron microscope.

Saito R., Tamura M., Matsui H., Nagano Y., Suzuki H., Kaneko T., Mizokami Y, & Hyodo I. (2014). Qing Dai attenuates nonsteroidal anti-inflammatory drug-induced mitochondrial reactive oxygen species in gastrointestinal epithelial cells. Journal of Clinical Biochemistry and Nutrition, 56(1), 8-14.

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