Mouse igg1 isotype control antibody clone mopc 21

Manufactured by BioXCell

Sourced in United States

The Mouse IgG1 isotype control antibody (clone MOPC-21) is a laboratory reagent used as a control in immunoassays and flow cytometry experiments. It binds to target antigens in a non-specific manner, allowing researchers to distinguish specific antigen-antibody interactions from background signals.

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Lab products found in correlation

Sterile pbs, by Thermo Fisher Scientific (2 mentions) N methylpyrrolidone, by Merck Group (2 mentions) Dodecyl isocyanate, by Merck Group (2 mentions) Hypromellose, by Merck Group (2 mentions) Histidine tagged human il 12, by Sino Biological (2 mentions) Anti il 17a clone 17f3, by BioXCell (1 mentions) Vancomycin, by Merck Group (1 mentions) Clindamycin, by Merck Group (1 mentions) Neomycin, by Merck Group (1 mentions) Mouse igg2a, by Southern Biotech (1 mentions) Mouse igg2b, by Southern Biotech (1 mentions)

Close spelling variants of this product

MOPC-21 (71 citations) Isotype control (29 citations) Isotype control antibody (27 citations) Clone MOPC-21 (21 citations) Mouse IgG1 isotype control (clone MOPC-21, (17 citations) Mouse IgG1 isotype control (13 citations) Mouse IgG1 (13 citations) Mouse IgG1 (clone MOPC-21; (7 citations) IgG1 isotype control (5 citations) IgG1 (MOPC-21; (4 citations)

7 protocols using mouse igg1 isotype control antibody clone mopc 21

LCMV-Docile Mice Anti-IFN-αR1 Treatment

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LCMV‐Docile chronic infected mice were treated with anti‐IFN‐αR1 (clone MAR1‐5A3) or mouse IgG1 isotype control antibody (clone MOPC21; Bioxcell, NH, USA) on day 4 (1 mg) and day 2 (500 μg) before VSV infection for histological analysis and additionally on day 5 (250 μg) for neutralizing antibody measurement.

Honke N., Shaabani N., Merches K., Gassa A., Kraft A., Ehrhardt K., Häussinger D., Löhning M., Dittmer U., Hengel H., Recher M., Lang P.A, & Lang K.S. (2015). Immunoactivation induced by chronic viral infection inhibits viral replication and drives immunosuppression through sustained IFN‐I responses. European Journal of Immunology, 46(2), 372-380.

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Experimental Autoimmune Encephalomyelitis Induction

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EAE induction was previously described. Briefly, age- (8–12 wk) and sex-matched mice were immunized with 200 μg MOG_35–55 peptide (Scrum) and complete Freund’s adjuvant containing 5 mg/ml (0.5 mg/mouse) heat-killed Mycobacterium tuberculosis (BD). Mice were injected with this emulsion at the tail base. Pertussis toxin (500 ng/mouse) was injected intraperitoneally on days 0 and 2. For IL-17A neutralization study, mice were treated intraperitoneally with 100 μg of anti-IL-17A (clone 17F3; BioXCell) or mouse IgG₁ isotype control antibody (clone MOPC-21; BioXCell) every 3 d, starting at day −1 of EAE immunization until day 14. Immunized mice were assessed everyday by clinical scores as follows: no clinical signs, 0; partially limp tail, 0.5; completely limp tail, 1; hind leg inhibition (hind legs fall when mice are dropped on a wire rack), 1.5; dragging of one hind leg, 2; dragging of both hind legs or paralysis of one hind leg, 2.5; paralysis of both hind legs, 3; paralysis of both hind legs and hind legs are together on one side of the body, 3.5; limp tail, complete hind legs, and mouse is minimally moving around the cage, 4; mouse is found dead due to paralysis, 5.

Miyashita Y., Kouwaki T., Tsukamoto H., Okamoto M., Nakamura K, & Oshiumi H. (2021). TICAM-1/TRIF associates with Act1 and suppresses IL-17 receptor–mediated inflammatory responses. Life Science Alliance, 5(2), e202101181.

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Mouse Model of C. difficile Infection

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Mice were cohoused for three weeks prior to antibiotic treatment then supplemented with neomycin (Sigma) (0.25 g/L) and vancomycin (Novaplus) (0.25 g/L) in the drinking water for 3 days. One day following cessation of antibiotic water, mice received 200 μg of clindamycin (Sigma) by i.p. injection. Twenty-four hours later, mice received 200 C. difficile spores (VPI10463 strain ATCC# 43255) via oral gavage. For IL-17a or IFN-γ blockade experiments, mice received 400 μg of anti-mIL17a antibody (clone 17F3 BioXcell), anti-mIFN-γ antibody (clone XMG1.2 BioXcell) or mouse IgG1 isotype control antibody (clone MOPC-21 BioXcell) i.p. every other day starting at day −1 of infection. After infection, mice were monitored and scored for disease severity by four parameters: weight loss (>95% of initial weight = 0, 95-90% initial weight = 1, 90-80% initial weight = 2, <80% = 3), surface body temperature (>32°C = 0, 32-30°C = 1, 30-28°C = 2, <28°C = 3), diarrhea severity (fo rmed pellets = 0, loose pellets = 0, liquidly discharge = 2, no pellets/caked to fur = 3), morbidity (score of 1 for each symptoms with max score of 3; ruffled fur, hunched back, lethargy, ocular discharge).

Abt M.C., Lewis B.B., Caballero S., Xiong H., Carter R.A., Sušac B., Ling L., Leiner I, & Pamer E.G. (2015). Innate Immune Defenses Mediated by Two ILC Subsets are Critical for Protection Against Acute Clostridium difficile Infection. Cell host & microbe, 18(1), 27-37.

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Lipid-Based Nanoparticle Protocol

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All lipids were purchased from Avanti Polar Lipids. See Supplemental Table 1 for a complete list of lipids and their associated catalog numbers. Hypromellose, N-methylpyrrolidone (NMP), and dodecyl isocyanate were purchased from Sigma Aldrich. Dialysis tubing was purchased from Spectrum Labs. Sterile PBS was purchased from Thermo Fisher. Tag-free and histidine tagged GFP were purchased from MilliporeSigma and Sino Biologicals, respectively. Mouse IgG1 isotype control antibody (clone MOPC-21) was purchased from Bio X Cell. Tag-free and histidine tagged human IL-12 were both purchased from Sino Biologicals.

Correa S., Grosskopf A.K., Klich J.H., Hernandez H.L, & Appel E.A. (2022). Injectable Liposome-based Supramolecular Hydrogels for the Programmable Release of Multiple Protein Drugs. Matter, 5(6), 1816-1838.

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Antibody-mediated IFN-γ and IL-17A Blockade

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Anti-IFN-γ (clone XMG1.2) was a generous gift from Mary Ann Accavitti-Loper (University of Alabama-Birmingham). Rat IgG1 isotype control antibody (clone HRPN), anti-IL-17A (clone 17F3), and mouse IgG1 isotype control antibody (clone MOPC-21) were purchased from BioXCell (West Lebanon, NH). Mice received 500 μg of anti-IFN-γ or rat IgG1 isotype control antibody or 100 ug of anti-IL-17A or mouse IgG1 antibody in 200 μL PBS via intraperitoneal injection on days 0, 2, and 4 post-inoculation.

Roberts L.M., Davies J.S., Sempowski G.D, & Frelinger J.A. (2014). IFN-γ, but not IL-17A, is required for survival during secondary pulmonary Francisella tularensis Live Vaccine Stain infection. Vaccine, 32(29), 3595-3603.

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Recombinant Protein Reagents for PD-1 and B7-1 Signaling

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Recombinant proteins human B7–1-hIgG1 and human PD-1-hIgG1 were purchased from R & D systems. Human IgG was purchased from Jackson and BioXcell. Mouse IgG1 isotype control antibody (clone MOPC21) was purchased from BioXcell. Mouse IgG2b and Mouse IgG2a were purchased from Southern Biotech. hPD-1-mIgG2a and hB7–1-mIgG2a were purchased from Chimerigen. hPD-L1-mIgG2a was made in our laboratory (14 (link)).

Chaudhri A., Xiao Y., Klee A.N., Wang X., Zhu B, & Freeman G.J. (2018). PD-L1 binds to B7-1 only in cis on the same cell surface. Cancer immunology research, 6(8), 921-929.

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Lipid-Based Nanoparticle Formulation Protocol

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All lipids were purchased from Avanti Polar Lipids. See Supplemental Table 1 for a complete list of lipids and their associated catalog numbers. Hypromellose, Nmethylpyrrolidone (NMP), and dodecyl isocyanate were purchased from Sigma Aldrich. Dialysis tubing was purchased from Spectrum Labs. Sterile PBS was purchased from Thermo Fisher. Tag-free and histidine tagged GFP were purchased from MilliporeSigma and Sino Biologicals, respectively. Mouse IgG1 isotype control antibody (clone MOPC-21) was purchased from Bio X Cell. Tag-free and histidine tagged human IL-12 were both purchased from Sino Biologicals.

Correa S., Grosskopf A.K., Klich J.H., Hernandez H.L., & Appel E.A. (2021). Injectable Liposome-based Supramolecular Hydrogels for the Programmable Release of Multiple Protein Drugs.

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