Porcine specific elisa kits

According to the protocol described by Wang et al. (16 (link)), samples (~0.1000 g) of the jejunal and ileal mucosa were mixed with PBS (1:9, w:v) and homogenized, and the supernatants were obtained by centrifugation at 2000 × g for 10 min at 4°C. The concentrations of sIgA, IL-6, IL-10, IL-17, IFN-γ, TNF-α, and TGF-β were measured using commercially available porcine-specific ELISA kits (Jiangsu Meimian Industrial Co., Ltd.). A Pierce BCA Protein Assay Kit (Thermo Scientific, Shanghai, China) was used to quantify the total protein concentration in the jejunal and ileal mucosa. The concentration of each index was normalized to the total protein in each sample. Absorbance was measured using a Multiscan Spectrum Spectrophotometer (Infinite M200Pro, Tecan).

The plasma concentrations of interleukin (IL)-1β, -6, -8, -10, tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β, and interferon (IFN)-γ were determined using commercially available porcine-specific ELISA kits from Jiangsu Meimian Industrial Co., Ltd. (Meimian, Yancheng, China) according to the manufacturer’s instructions. Absorbance values were measured using a Multiscan Spectuum Spectrophotometer (Infinite M200Pro, Tecan, Switzerland).