The interactions between LysP108 and MRSA (XN108 and SCCmec V) strains were assessed in vitro. LysP108 and bacteria cells were suspended in 10 mM PBS (pH 7.4). Then, 200 μL of LysP108 (250 μg mL-1) and 800 μL of the test bacteria (108 CFU mL-1) were mixed together and incubated at 37°C for 5, 30, and 60 min, respectively. For the complete removal of free LysP108, the mixtures were then centrifuged at 5000 g for 5 min and washed thrice by PBS (10 mM; pH 7.4). Subsequently, the sediments comprising bacteria with LysP108 were suspended in PBS (10 mM; pH 7.4). The morphology of aggregated pellets were detected under a scanning electron microscope (SEM, Inspect F, Philips, The Netherlands).
Inspect f
Manufactured by Philips
The Inspect F is a laboratory equipment product designed for visual inspection. It provides a controlled and consistent environment for examining samples or materials.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using inspect f
1
Interactions of LysP108 with MRSA Strains
2
Extensive Characterization of Ceramic Materials
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Phase analysis for all samples was performed using a Panalytical X'Pert X-ray diffractometer. High resolution XRD patterns for lattice parameter determination were obtained using a Stoe Cu-PSD (position sensitive detector).
Ceramics for SEM were first thermally etched at 90 % of the sinter temperature for 30 minutes. The calcined powders and sintered, thermally etched ceramics were affixed to carbon pads, sputtered with gold, and imaged using a Phillips Inspect F. Energy dispersive Xray analysis was performed using an EDAX EDS detector.
Infrared spectroscopy was performed using a Perkin Elmer Frontier FTIR and GoldenGate Diamond ATR between 4000-400 cm -1 . Samples for FTIR were dried at 180 °C for 24 h before testing to remove surface H2O and CO2. Measurements were taken with the CO2/H2O suppression off, which resulted in some noise in the data (2500-1250 cm -1 ).
Raman spectroscopy was performed using a Renishaw inVia Raman microscope with a 514 nm green laser operated at 20 mW.
Gold paste electrodes were applied to sintered ceramics for electrical testing. Bulk capacitance and tan h (the dissipation factor expressed as the ratio of equivalent series resistance to capacitive resistance) were measured using an LCR meter (Model 4284A, Hewlett Packard, HP). Measurements were taken at the following fixed frequencies: 100 kHz, 250 kHz and 1 MHz at temperatures between 25 and 250 °C.
Ceramics for SEM were first thermally etched at 90 % of the sinter temperature for 30 minutes. The calcined powders and sintered, thermally etched ceramics were affixed to carbon pads, sputtered with gold, and imaged using a Phillips Inspect F. Energy dispersive Xray analysis was performed using an EDAX EDS detector.
Infrared spectroscopy was performed using a Perkin Elmer Frontier FTIR and GoldenGate Diamond ATR between 4000-400 cm -1 . Samples for FTIR were dried at 180 °C for 24 h before testing to remove surface H2O and CO2. Measurements were taken with the CO2/H2O suppression off, which resulted in some noise in the data (2500-1250 cm -1 ).
Raman spectroscopy was performed using a Renishaw inVia Raman microscope with a 514 nm green laser operated at 20 mW.
Gold paste electrodes were applied to sintered ceramics for electrical testing. Bulk capacitance and tan h (the dissipation factor expressed as the ratio of equivalent series resistance to capacitive resistance) were measured using an LCR meter (Model 4284A, Hewlett Packard, HP). Measurements were taken at the following fixed frequencies: 100 kHz, 250 kHz and 1 MHz at temperatures between 25 and 250 °C.
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