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Anti mouse il 6

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Anti-mouse IL-6 is a laboratory reagent used to detect and quantify the levels of interleukin-6 (IL-6) in mouse samples. It is a specific antibody that binds to the IL-6 protein, allowing for its identification and measurement.

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Lab products found in correlation

Crystal violet, by Merck Group (1 mentions) Ruxolitinib, by Bio-Techne (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions) Dnase 1, by Roche (1 mentions) Collagenase 1, by Worthington (1 mentions) Anti mouse ly6g, by BioXCell (1 mentions) G csf elisa kit, by R&D Systems (1 mentions) Corticosterone elisa, by R&D Systems (1 mentions) Murine il 6, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

InVivoMab anti-mouse IL-6

3 protocols using anti mouse il 6

1

Matrigel-based Cell Culture Assay

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Growth factor reduced Matrigel Matrix (MG) was obtained from BD Biosciences. Collagenase I was purchased from Worthington and DNAse I from Roche. BSA, crystal violet (CV), and paraformaldehyde (PFA) were obtained from Sigma-Aldrich. Dox and MTX were provided by Dr. Khalil Zaman from the Department of Oncology, University Hospital, University of Lausanne, Lausanne, Switzerland. Ruxolitinib (JAK inhibitor, catalog 7064) was purchased from Bio-Techne and used at 5 μg/ml. The anti-mouse OSM (R&D systems Clone: P53347), anti-mouse IL-6 (BioxCell, Clone MP5-20F3) were used at 1 μg/ml. The recombinant mouse OSM and IL-6 (BioLegend, catalog 762802 and 575702, respectively) were both used at 10 ng/ml.
Peyvandi S., Bulliard M., Yilmaz A., Kauzlaric A., Marcone R., Haerri L., Coquoz O., Huang Y.T., Duffey N., Gafner L., Lorusso G., Fournier N., Lan Q, & Rüegg C. (2024). Tumor-educated Gr1+CD11b+ cells drive breast cancer metastasis via OSM/IL-6/JAK–induced cancer cell plasticity. The Journal of Clinical Investigation, 134(6), e166847.
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2

Cytokine and Stress Hormone Analysis

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Anti-mouse IL-6 (Bio-X-Cell, BE0046) and anti-mouse Ly6G (Bio-X-Cell, BE0075–1); G-CSF ELISA kit (R&D Systems, MCS00); Corticosterone ELISA (R&D Systems, KGE009); IL-6 ELISA (Biolegend, 431304); Histamine ELISA (Beckman Coulter, IM2015).
Cronin S.J., Tejada M.A., Song R., Laval K., Cikes D., Ji M., Brai A., Stadlmann J., Novatchikova M., Perlot T., Ali O.H., Botta L., Decker T., Lazovic J., Hagelkruys A., Enquist L., Rao S., Koyuncu O.O, & Penninger J.M. (2023). Pseudorabies virus hijacks DDX3X, initiating an addictive “mad itch” and immune suppression, to facilitate viral spread. bioRxiv.
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3

Murine Model for IL-6 Modulation in UTI

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For systemic administration in the absence of UTI, 100 ng murine IL-6 (Peprotech, Rocky Hill, NJ) or PBS/0.1% bovine serum albumin carrier was injected intraperitoneally in 100 μl total volume. For local administration during UTI, 100 ng IL-6 or carrier was injected intravesically in 50 μl one hour before UPEC inoculation and repeated 2 hours after inoculation. For neutralization, female C3H/HeOuJ mice received an intraperitoneal injection of 1 mg anti-mouse IL-6 (BioXCell, clone MP5-20F3, in 200 μl) or rat IgG1 isotype control (BioXCell, clone HRPN) 24 hours prior to UPEC infection. The injection was repeated 1, 3, and 6 dpi.
Ching C.B., Gupta S., Li B., Cortado H., Mayne N., Jackson A.R., McHugh K.M, & Becknell B. (2018). Interleukin-6/Stat3 signaling has an essential role in the host antimicrobial response to urinary tract infection. Kidney international, 93(6), 1320-1329.
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