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2 protocols using foxj1 hfh4

1

Isolation and Characterization of Airway Epithelial Cells

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Collagenase type A (Roche, Basel, Switzerland); Dispase II (Gibco/ThermoFisher, Waltham, MA); Elastase (Worthington-Biochem, Lakewood, NJ); DNAase (Sigma-Aldrich, St. Louis, MO); Absolute RNA Microprep kit (Agilent, #400805, Stratagene, La Jolla, CA); bronchial epithelial basal medium (BEBM, Lonza, Mapleton, IL); small airway epithelial cell growth medium (SAGM; Lonza); Dulbecco’s modified Eagle medium (DMEM; GIBCO, Rockville, MD); PneumaCult-ALI medium (Stemcell Technologies, Vancouver, Canada); iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA); SYBR Green (Applied Biosystems, Foster City, CA. SCGB1A1/CCSP (Secretoglobin family 1A, member 1) antibodies (LifeSpan BioScience, Seattle, WA); FOXJ1/HFH4 (Novus Biologicals, Littleton, CO).
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2

Isolation and Characterization of Airway Epithelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Collagenase type A (Roche, Basel, Switzerland); Dispase II (Gibco/ThermoFisher, Waltham, MA); Elastase (Worthington-Biochem, Lakewood, NJ); DNAase (Sigma-Aldrich, St. Louis, MO); Absolute RNA Microprep kit (Agilent, #400805, Stratagene, La Jolla, CA); bronchial epithelial basal medium (BEBM, Lonza, Mapleton, IL); small airway epithelial cell growth medium (SAGM; Lonza); Dulbecco’s modified Eagle medium (DMEM; GIBCO, Rockville, MD); PneumaCult-ALI medium (Stemcell Technologies, Vancouver, Canada); iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA); SYBR Green (Applied Biosystems, Foster City, CA. SCGB1A1/CCSP (Secretoglobin family 1A, member 1) antibodies (LifeSpan BioScience, Seattle, WA); FOXJ1/HFH4 (Novus Biologicals, Littleton, CO).
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