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Labsystems fluoroskan ascent fl

Manufactured by Thermo Fisher Scientific
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Sourced in Finland

The LabSystems Fluoroskan Ascent FL is a microplate fluorometer designed for sensitive fluorescence measurements. It provides precise and accurate measurement of fluorescent samples in microplates.

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Lab products found in correlation

Qcm 24 well colorimetric cell migration assay, by Merck Group (1 mentions) Celltox green cytotoxicity, by Promega (1 mentions) Apotox glo triplex assay kit, by Promega (1 mentions)

2 protocols using labsystems fluoroskan ascent fl

1

Cell Migration Assay Protocol

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Undifferentiated HK532 and HK532-IGF-I cells were added to migration inserts following overnight storage at 4°C (1 × 106 cells/ml or 3 × 106 cells per vial), or were alternatively cultured on six-well plates and moved to inserts on D7 of differentiation. NSDM plus 10% fetal bovine serum was added below the inserts. After 24 hours, cells that had migrated through the insert were stained using the QCM 24-well Colorimetric Cell Migration Assay (Millipore). Migration was quantified using a standard LabSystems Fluoroskan Ascent FL microplate reader (Thermo Fisher Scientific) at 530 and 590 nm.
McGinley L.M., Sims E., Lunn J.S., Kashlan O.N., Chen K.S., Bruno E.S., Pacut C.M., Hazel T., Johe K., Sakowski S.A, & Feldman E.L. (2016). Human Cortical Neural Stem Cells Expressing Insulin-Like Growth Factor-I: A Novel Cellular Therapy for Alzheimer’s Disease. Stem Cells Translational Medicine, 5(3), 379-391.
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2

Cytotoxicity and Apoptosis Assays for Drug Evaluation

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The CellTox Green Cytotoxicity and ApoTox-Glo Triplex assay kits (Promega, Madison, WI, USA) were used to measure cytotoxicity at different timepoints and to assess the mechanism of action of the drugs studied, respectively. In all, 104 cells per well were plated in 96-well microplates (black walls for CellTox assay and white for ApoTox), changing the medium to phenol red-free DMEM after 24 h. The protocols were carried out following the manufacturer's instructions, and fluorescence and luminescence were measured in a Labsystems Fluoroskan Ascent FL (Thermo, Vantaa, Finland) plate reader.
Martínez-Pérez C., Ward C., Turnbull A.K., Mullen P., Cook G., Meehan J., Jarman E.J., Thomson P.I., Campbell C.J., McPhail D., Harrison D.J, & Langdon S.P. (2016). Antitumour activity of the novel flavonoid Oncamex in preclinical breast cancer models. British Journal of Cancer, 114(8), 905-916.
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