The β-galactosidase (β-gal) assay was performed using the Senescence-β-Galactosidase Kit (Beyotime). Young and aging TSPCs were plated on 6-well plates and incubated for 48 h. Cells were incubated with the kit’s staining mixture for 16 h at 37 °C without CO2. The percentages of β-gal-positive cells were calculated by counting positive cells and all the cells in nine random microscopic fields. Images were captured by an Olympus CKX53 inverted phase-contrast microscope (× 4 objectives).
Ckx53 inverted phase contrast microscope
Manufactured by Olympus
The CKX53 inverted phase-contrast microscope is a laboratory equipment product designed for imaging and analysis. It features phase-contrast optics to enhance contrast in transparent samples. The microscope is intended for use in various research and testing applications.
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2 protocols using ckx53 inverted phase contrast microscope
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Senescence-associated β-galactosidase Assay
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Senescence-Associated β-Galactosidase Assay
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The β-galactosidase (β-gal) assay was performed using the SA-β-gal staining kit (Sigma). Cells were plated on 12-well plates and incubated for 48 h. Cells were incubated with the kit’s staining mixture for 16 h at 37 °C. The percentages of β-gal-positive cells were calculated by counting 300 cells in six microscopic fields. Images were captured by an Olympus CKX53 inverted phase-contrast microscope (×4 or ×10 objectives).
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