Phospho sting s366

Manufactured by Cell Signaling Technology

Phospho-STING S366 is a monoclonal antibody that detects endogenous levels of STING (Stimulator of Interferon Genes) when phosphorylated at serine 366. STING is a critical component of the innate immune response to cytosolic DNA.

Automatically generated - may contain errors

Lab products found in correlation

Gapdh, by Santa Cruz Biotechnology (2 mentions) Lamp2, by Santa Cruz Biotechnology (1 mentions) Gm130 antibody, by BD (1 mentions) Golgin 97, by Proteintech (1 mentions) Golgin97, by Santa Cruz Biotechnology (1 mentions) Lamp1 rabbit mab, by Cell Signaling Technology (1 mentions) Gm130 rabbit mab, by Cell Signaling Technology (1 mentions) F7425, by Merck Group (1 mentions) Flag m2, by Merck Group (1 mentions) Human recombinant tgfβ, by R&D Systems (1 mentions) Celllight golgi rfp, by Thermo Fisher Scientific (1 mentions) Quant it assay kit, by Thermo Fisher Scientific (1 mentions) Vdac1, by Novus Biologicals (1 mentions) Tom20, by Santa Cruz Biotechnology (1 mentions) Sting, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Phospho-STING STING

2 protocols using phospho sting s366

Antibody Characterization for Cellular Organelles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies for LAMP1 (sc-20011, IF 1:200); LAMP2 (sc-18822, IF 1:200); Golgin97 (sc-59820, IF 1:500); GAPDH (sc-365062, WB 1:5000); Tubulin (sc-5286, WB 1:3000) were from Santa Cruz Biotechnology. The GM130 antibody (610822, IF 1:1000) was from BD Biosciences. Flag (M2, IF 1:1000; F7425, WB 1:3000) antibody was from Sigma. LAMP1 rabbit mAb (#9091, IF 1:200); GM130 rabbit mAb (#12480, IF 1:300); Rab5 rabbit mAb (#3547, IF 1:200), Phospho-STING S366 (#50907, WB 1:1000); Phospho-TBK1 Ser172 rabbit mAb (#5483, WB/IF 1:1000) were from Cell Signaling. The following antibodies were from Proteintech: STING (19851-1-AP, WB 1:1000, IF 1:1000); LC3 (14600-1-AP, IF 1:1000, WB 1:1000, reacts with LC3A, LC3B, and LC3C); TGN46 (13573-1-AP, IF 1:1000); Golgin97 (12640-1-AP, IF 1:1000). Alexa-488/594- and Pacific Blue-conjugated secondary antibodies were obtained from ThermoFisher Scientific.

Xun J., Zhang Z., Lv B., Lu D., Yang H., Shang G, & Tan J.X. (2024). A conserved ion channel function of STING mediates noncanonical autophagy and cell death. EMBO Reports, 25(2), 544-569.

+ Open protocol

+ Expand

Investigating STING and IRF3 Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

STING (Cat# D2P2F), phospho-STING S366 (Cat# D7C36), IRF3 (Cat# D83B9),
and phospho-IRF3 S396 (Cat# D601M) were purchased from Cell Signaling
Technology; DNA (Cat# CBL186; 1:250) from Thermo Fisher; VDAC1 (Cat#
NB100–695) from Novus Biologicals; cGAS (Cat# sc-515777), TOM20 (Cat#
sc-11415), and GAPDH (Cat# sc-47724) from Santacruz Biotechnology; and
α-smooth muscle actin (Cat# MAB1420; 1:500) from R&D systems. Human
recombinant TGF-β was purchased from R&D systems (7754-BH). Inhibitor
for STING (Cat# IFM-004490–7) was provided by IFM therapeutics. The CGAS
inhibitor RU.521 (Cat# SML2347–5MG), 4-Dinitrophenol (Cat# D-198501),
SMAD inhibitor (Cat# SB53512), and zinc chelator N, N, N′,
N′-Tetrakis (2-pyridylmethyl) ethylenediamine TPEN (Cat# P-4413) were
purchased from Millipore Sigma. Golgi were labeled using CellLight Golgi-RFP
(Thermo Fisher Scientific, Cat# C10593). The CellLight reagent was directly
added to medium (2 μL/200 μL medium) before treatment. Picogreen
stain was from the Quant-IT assay kit (Thermo Fisher Scientific, Cat#
P7589).

Arumugam S., Li B., Boodapati S.L., Nathanson M.H., Sun B., Ouyang X, & Mehal W.Z. (2023). Mitochondrial DNA and the STING pathway are required for hepatic stellate cell activation. Hepatology (Baltimore, Md.), 78(5), 1448-1461.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!