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2 protocols using anti k48 ub

1

Subcellular Fractionation and Immunoblotting

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Cell lysates or chromatin-unbound and bound fractions were prepared and separated as described previously [22 (link), 23 (link)]. Antibodies against PAF1, LEO1, CDC73 and CTR9 were purchased from Bethyl Laboratories (Montgomery). Anti-LAMIN B, ACTIN, CYCLIN A, c-Myc, PARP1, GFP and H3 antibodies were purchased from Santa Cruz Biotechnology, anti-CNOT4 from Abcam, anti-GAPDH from Chemicon, anti-HA from Roche and anti-K48-UB from Millipore.
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2

Antibodies for Ciliopathy Protein Analysis

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Antibodies used in this study included anti-HA, anti-NPHP5, anti-Myc, anti-MARCH7, anti-γ tubulin-FITC (Santa Cruz); anti-Flag, anti-GFP, anti-β actin, (Sigma); anti-CP110, anti-USP9X, anti-CEP290 (Bethyl Laboratories); anti-BBS11 (Thermo Fisher), anti-centrin, anti-K48 Ub (Millipore); anti-K63 Ub (Abcam); anti-Ub (Dako); anti-IFT88 (Proteintech); anti-glutamylated tubulin GT335, anti-acetylated tubulin and anti-Ki67 (Invitrogen). Two additional anti-NPHP5 antibodies (IRCM1 and IRCM2) were also used[25 (link)].
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