Winepr program

Manufactured by Bruker

The WinEPR program is a software package developed by Bruker for the acquisition and analysis of electron paramagnetic resonance (EPR) data. It provides a comprehensive suite of tools for the control and operation of Bruker EPR spectrometers, as well as for the processing and interpretation of EPR spectra.

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Lab products found in correlation

Emx 20 2.7 epr spectrometer x band, by Bruker (2 mentions) Emxplus epr spectrometer, by Bruker (1 mentions)

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WinEPR SimFonia program (3 citations)

7 protocols using winepr program

Hydroxyl Radical Detection by EPR

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Dimethyl pyridine N oxide (DMPO) was used as a free radical trapper. EPR signals were detected with a Bruker e‐scan EPR spectrometer (Burker, Karlsruhe, Germany) as previous report (Ohsawa et al., 2007). We produced hydroxyl radical (OH˙) by the Fenton reaction in the mixture of 0.25 mm H₂O₂ and 0.1 mm FeSO₄ in the presence of 0.1 mm DMPO. The spin traps, DMS (0.1, 1, or 10 mm), and MsrA/nonactive MsrA (1 μm) were added before the Fe (II) and H₂O₂. Samples (20 μL) were loaded into a quartz tube, and the EPR spectra were recorded at room temperature. The EPR microwave power was set to 4.88 mW. The modulation frequency was 9.76 GHz. The time constant was 81.92 ms, and conversion time was 81.92 ms. A sweep time of 41.94 s was used. Each sample was scanned once. A sweep width of 100 G was used for experiments with DMPO. The receiver gain was set to 3.17 × 10³. Simulation and fitting of the EPR spectra were performed using the Bruker WinEPR program.

Guan X., Wu P., Wang S., Zhang J., Shen Z., Luo H., Chen H., Long L., Chen J, & Wang F. (2016). Dimethyl sulfide protects against oxidative stress and extends lifespan via a methionine sulfoxide reductase A‐dependent catalytic mechanism. Aging Cell, 16(2), 226-236.

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EPR Analysis of Tobacco Smoke Particulate

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Smoke particulate from the burning of tobacco cigarette (SM1) trapped in situ on silica and immediately analyzed using EPR. About 5 mg sample of SM1 tobacco particulate adsorbed on silica gel was analyzed using a Bruker EMX-20/2.7 EPR spectrometer (X-band) with dual cavities, modulation and microwave frequencies of 100 kHz and 9.516 GHz, respectively [21 (link), 4 (link)]. The characteristic parameters were: sweep width of 200 G, EPR microwave power of 1–20 mW, and modulation amplitude of ≤4 G. The time constant was varied appropriately. The sweep time was set at 84 s and the number of scans was fixed at 10. The receiver gain for this investigation was 50. The g-value was computed using Bruker’s WINEPR program, which is a comprehensive line of software, allowing control of the Bruker EPR spectrometer, data acquisition, automation routines, tuning, and calibration programs on a Windows-based PC [22 , 4 (link)]. The exact g-value for the key spectrum was determined by comparing with a 2,2-diphenyl-1-picrylhydrazyl (DPPH) standard [23 (link)]. Constituent radicals in a mixture can only be distinguished by judicious variation of the experimental conditions (temperature, pressure, and annealing parameter procedures) followed by computer analysis of digitally stored spectra [21 (link)] which is beyond the scope of the current investigation.

Jebet A., Kibet J., Ombaka L, & Kinyanjui T. (2017). Surface bound radicals, char yield and particulate size from the burning of tobacco cigarette. Chemistry Central Journal, 11, 79.

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EPR Analysis of Biodiesel-Diesel Char

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About 5 mg thermal char sample from the co-pyrolysis of biodiesel and commercial diesel was analyzed using a Bruker EMX-20/2.7 EPR spectrometer (X-band) with dual cavities, modulation and microwave frequencies of 100 kHz and 9.516 GHz, respectively [26 (link), 27 (link)]. The typical parameters were: sweep width of 200 G, EPR microwave power of 1–20 mW, and modulation amplitude of ≤ 6 G. Time constant and sweep time were 16 s and 84 s, respectively. The value of the g factors was calculated using Bruker’s WINEPR program, which is a comprehensive line of software that allows control of the Bruker EPR spectrometer, data-acquisition, automation routines, tuning, and calibration programs on a windows-based personal computer [28 (link)]. The actual g-value for the spectrum was estimated by comparison with a 2,2-diphenyl-1-picrylhydrazyl (DPPH) standard.

Kibet J.K., Mosonik B.C., Nyamori V.O, & Ngari S.M. (2018). Free radicals and ultrafine particulate emissions from the co-pyrolysis of Croton megalocarpus biodiesel and fossil diesel. Chemistry Central Journal, 12, 89.

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Quantification of EPR-Active Metals

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Quantification of EPR-active metal complexes was achieved by calculating the double integration values of iron/manganese with and without DFO using WinEPR program (Bruker Biospin Corp., Billerica, MA). Concentrations were determined through the use of calibration curves consisting of standard solutions of Fe³⁺-DFO or Mn(ClO₄)₂.6H₂O. One-way analysis of variance (ANOVA) was used to determine the breadth of statistical difference in our data. P-values > 0.05 were considered non-significant.
The amount of sequestered iron was determined by subtracting the Fe³⁺-DFO concentration (available Fe) from the total Fe intracellular concentration. For determining redox speciation of the available Fe pool, the concentration of available Fe³⁺ was measured directly from the EPR signal prior to DFO treatment. The available Fe²⁺ was determined by subtracting this value from the total available Fe³⁺-DFO.
For the redox speciation of Mn, the amount of intracellular Mn²⁺ was measured directly from the EPR signal prior to DFO treatment. The amount of intracellular Mn^3+/4+ was determined by subtracting this value from the total intracellular Mn content (from ICP-OES). For cellular speciation of Mn²⁺, the amount of sequestered Mn²⁺ was measured directly from the EPR signal after treatment with DFO, and the available Mn²⁺ was determined by subtracting this value from the total intracellular Mn²⁺ content.

Murgas C.J., Green S.P., Forney A.K., Korba R.M., An S.S., Kitten T, & Lucas H.R. (2020). Intracellular Metal Speciation in Streptococcus sanguinis Establishes SsaACB as Critical for Redox Maintenance. ACS infectious diseases, 6(7), 1906-1921.

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Electron Paramagnetic Resonance Characterization

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Electron paramagnetic resonance (EPR) X-band (9.8 GHz) was recorded at room temperature on a Bruker ElexSys E500 instrument equipped with an NMR teslameter ER 036TM and E 41 FC frequency counter. The concentration of spins was measured using a double integral procedure applying the Bruker WinEPR program. Leonardite was used as a spin concentration standard (International Humic Substance Society) [64 (link)].

Szajdak L.W., Jezierski A., Wegner K., Meysner T, & Szczepański M. (2020). Influence of Drainage on Peat Organic Matter: Implications for Development, Stability, and Transformation. Molecules, 25(11), 2587.

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EPR Spectroscopy of Cyanide Toxicity

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EPR was performed using a Bruker EMX-plus EPR spectrometer (Bruker, Germany) operated at 9.4 GHz. CYA or its metabolites and 50 μl of PBN or DMPO were pre-degassed separately with N₂ gas. Two hundred microliters of CYA (1 mg/ml) was added with 2 ml of bacterial cells, extracted bacterial proteins, or XO/X respectively in tubes flushed with N₂ and the mixtures were maintained in an oil bath at 37°C for 15 min. The EPR spectra were monitored at the following conditions: microwave frequency, 9.4 GHz; microwave power, 18 mW; modulation amplitude, 3.0 G; field sweep from 3000 to 3800 G; time constant, 80 ms; conversion time, 30 ms. The spectra were simulated using the WinEPR program (Bruker).

Cheng G., Li B., Wang C., Zhang H., Liang G., Weng Z., Hao H., Wang X., Liu Z., Dai M., Wang Y, & Yuan Z. (2015). Systematic and Molecular Basis of the Antibacterial Action of Quinoxaline 1,4-Di-N-Oxides against Escherichia coli. PLoS ONE, 10(8), e0136450.

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EPR Analysis of Hydroxyl Radical Scavenging

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EPR was performed as described in our previous reports with some modifications [28, 29] . DMPO was used as a free radical trapper. EPR signals were detected with a Bruker e-scan EPR spectrometer (Burker, Germany). We produced hydroxyl radical (OH•) by the Fenton reaction in the mixture of 0.25 mmol/L H 2 O 2 and 0.1 mmol/L FeSO 4 in the presence of 0.1 mmol/L DMPO. The spin traps, L-Met (0.1 or 1 mmol/L), SMLC (0.1, 1 or 10 mmol/L) and rMsrA/non-active rMsrA (1 µmol/L) were added before the Fe (II) and H 2 O 2 . Samples (20 µL) were loaded into a quartz tube and the EPR spectra were recorded at room temperature. The EPR microwave power was set at 4.88 mW. The modulation frequency was 9.76 GHz. The time constant was 81.92 ms. The conversion time was 81.92 ms and a sweep time of 41.94 s was used. Each sample was scanned once. A sweep width of 100 G was used for experiments with DMPO. The receiver gain was set at 3.17 × 10 3 . Simulation and fitting of the EPR spectra were performed using the Bruker WinEPR program.

Ni L., Guan X.L., Chen F.F, & Wu P.F. (2020). S-methyl-L-cysteine Protects against Antimycin A-induced Mitochondrial Dysfunction in Neural Cells via Mimicking Endogenous Methionine-centered Redox Cycle. Current medical science, 40(3).

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