Isomet slow speed bone cutter

Manufactured by Buehler

Sourced in United States

The Isomet-Slow Speed Bone Cutter is a precision laboratory equipment designed for cutting and sectioning of hard materials, such as bone samples. It operates at a slow speed to ensure a clean and controlled cut without damaging the specimen.

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Lab products found in correlation

Lsm 510 meta, by Zeiss (2 mentions) Tcs sp8, by Leica (2 mentions) Carl lsm 510 meta confocal microscope, by Zeiss (1 mentions) Qwin software, by Leica (1 mentions)

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Isomet (286 citations)

6 protocols using isomet slow speed bone cutter

Femoral Drill-Hole Injury Model in Rats

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Example 13

Drill-Hole Injury in the Femur:

A drill-hole was created in adult rats by inserting a drill bit with a diameter of 0.8 mm in the anterior portion of the diaphysis of femur, 2 cm above the knee joint. Treatments were given for 12 days prior to termination. 24 h before termination, calcein, a bone-seeking fluorochrome was administered to each rats (20 mg/kg, i.p.) to measure new bone formation at the fractured callus. On the 12^thday, rats were killed and femurs were collected and stored in 70% isopropanol for 48 h, and embedded in an acrylic material. Sections (60 μm) through the fracture callus were made using Isomet-Slow Speed Bone Cutter (Buehler, Lake Bluff, Ill.) followed by photography using confocal microscope (LSM 510 Meta, Carl Zeiss, Inc., Jena, Germany) with appropriate filters. The intensity of calcein binding was calculated using Carl Zeiss AM 4.2 image-analysis software.

US11304927B2. Bioactive extract, fraction of Cassia occidentalis and formulation thereof for bone regeneration (2022-04-19). COUNCIL OF SCIENTIFIC AND INDUSTRIAL RESEARCH [IN]. Inventors: Naibedya Chattopadhayay [IN], Subhashis Pal [IN], Sudhir Kumar [IN], Ramakrishna Eppalapally [IN], Padam Kumar [IN], Sapana [IN], Jiaur Rahaman Gayen [IN], Riyazuddin Mohammed [IN], Sabyasachi Sanyal [IN], Anagha Gurjar [IN], Prabhat Ranjan Mishra [IN], Naresh Mittapelly [IN], Kamal Ram Arya [IN], Brijesh Kumar [IN], Srikanta Rath [IN], Arun Kumar Trivedi [IN], Maurya Rakesh [IN].

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Bone Healing Assessment in Rat Model

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Forty-two female SD rats (220 ± 20 g, three months old, sexually mature) were taken, and drill holes of 0.8 mm were made at the femoral mid-diaphysis according to our previously published protocol (18 (link), 19 (link)). Postsurgery, rats were divided randomly in the presence of two researchers into seven groups (n = 6 rat/group); vehicle (water), USKECSE (100 mg/kg, 150 mg/kg, 200 mg/kg), USCSE (100 mg/kg, 150 mg/kg, 200 mg/kg). All treatments were given orally daily for 12 days. All animals received a subcutaneous (s.c.) injection of calcein (20 mg/kg) 24 hours before sacrifice. For the calcein labeling studies, bones were embedded in acrylic material, and 60 μm sections through the osteotomy site were made using an Isomet-Slow Speed Bone Cutter (Buehler, Lake Bluff, IL) (19 (link)). Sections were photographed using a confocal microscope (Leica TCS SP-8, Wetzlar, Germany) and analyzed using LAS-X software.

Kulkarni C., Sharma S., Bora P.S., Verma S., Rajput S., Porwal K., Rath S.K., Gayen J.R., Sharma U, & Chattopadhyay N. (2022). A novel extraction method enhanced the osteogenic and anti-osteoporosis effect of tea extract without any hepatotoxicity in ovariectomized rats. Frontiers in Endocrinology, 13, 951800.

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Femur Osteotomy Bone Regeneration Model

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Adult rats with a drill-hole (0.8mm) osteotomy in the femur diaphysis provide a rapid and reliable model for evaluating bone regeneration that is proportional to bone formation (17 (link), 18 (link)). Furthermore, this model is useful for the determination of effective osteogenic dose of a drug.
Twenty four female SD rats (220 ± 20 g) were used for femur osteotomy following a previously described protocol (19 (link)). Post-surgery, rats were randomly divided into four groups (n=6 rats/group); vehicle (water, orally), CFE (25-, 50- and 100 mg/kg, orally). All the treatments were given daily for 12 days. 24 h before sacrifice, all the animals were given subcutaneous (s.c.) injections of calcein (20 mg/kg). After sacrifice, bones were collected and processed for calcein labeling studies according to our previously published protocol. 60 μm sections were made through the osteotomy site using Isomet-Slow Speed Bone Cutter (Buehler, Lake Bluff, IL, USA) (19 (link), 20 (link)). Sections were photographed using a confocal microscope (Leica TCS SP-8, Wetzlar, Germany) and analyzed using LAS-X software.

Kulkarni C., Sharma S., Porwal K., Rajput S., Sadhukhan S., Singh V., Singh A., Baranwal S., Kumar S., Girme A., Pandey A.R., Singh S.P., Sashidhara K.V., Kumar N., Hingorani L, & Chattopadhyay N. (2023). A standardized extract of Coleus forskohlii root protects rats from ovariectomy-induced loss of bone mass and strength, and impaired bone material by osteogenic and anti-resorptive mechanisms. Frontiers in Endocrinology, 14, 1130003.

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Liraglutide Enhances Fracture Healing

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For experiments on fracture healing, 30 SD rats (200 ± 20 g) were divided into 3 groups and drill-holes were made by using a drill bit of 0.8 mm at femoral mid-diaphysis according to our previously published protocol [14 (link)]. One day after surgery, animals were divided into three equal groups; one group received vehicle (water), other two received Lira (0.3- or 0.6 mg/kg s.c.) for 12 days.
For studies in OVX rats, 20 adult female SD rats (250 ± 30 g, 8–10 months) underwent bilateral OVX. All animals were left untreated for osteopenia to develop which was confirmed by live animal μCT scan after 12 weeks. After confirming significant femoral bone loss, drill-hole was made as described above. 0.3 mg/kg Lira was administered subcutaneously for 12 days.
Calcein (20 mg/kg) was injected 24 h before sacrificing each animal to measure new bone formation at the drill-hole site. After the autopsy, all bones were frozen at −20 °C for μCT and microscopic analyses [15 (link)].
Bones were cleaned to remove soft tissue and were embedded in acrylic material. 50 μm sections through the drill-hole were prepared using Isomet-slow speed bone cutter (Buehler, Lake Bluff, IL) followed by confocal microscopy (Carl Zeiss LSM 510 meta confocal microscope, Zeiss Germany) to determine calcein intensity.

Pal S., Maurya S.K., Chattopadhyay S., China S.P., Porwal K., Kulkarni C., Sanyal S., Sinha R.A, & Chattopadhyay N. (2019). The osteogenic effect of liraglutide involves enhanced mitochondrial biogenesis in osteoblasts. Biochemical pharmacology, 164, 34-44.

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Quantitative Bone Histomorphometry Analysis

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Cross-sections (50 μm thickness) of periosteal regions of undecalcified diaphysis tibial bone of each mouse were obtained using an Isomet-Slow Speed Bone Cutter (Buehler, Lake Bluff, IL, USA). Images were captured using Leica-Qwin software (Leica Microsystems Inc., Buffalo Grove, IL, USA) and bone forming rate/bone surface (BFR/BS) and MAR were calculated. Tetracycline was injected 30 (20 mg/kg) days before termination of treatment, whereas calcien (20 mg/kg) 24 h before termination of treatment.^{36 (link)} For TRAP histochemistry, femur bones were fixed in 4% formaldehyde and decalcified using EDTA solution. The bones were dehydrated, sectioned in 5 μm thick size, deparaffined and TRAP staining was performed according to previously published protocol. Histological section of bone stained for TRAP activity was analyzed with bioquant software. Analysis of osteoclast number and osteoclast surface was performed according to standardized protocols of the American Society for Bone and Mineral.^{38 (link)}

Kushwaha P., Khedgikar V., Gautam J., Dixit P., Chillara R., Verma A., Thakur R., Mishra D.P., Singh D., Maurya R., Chattopadhyay N., Mishra P.R, & Trivedi R. (2014). A novel therapeutic approach with Caviunin-based isoflavonoid that en routes bone marrow cells to bone formation via BMP2/Wnt-β-catenin signaling. Cell Death & Disease, 5(9), e1422-.

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Dynamic Histomorphometry of Femora

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For dynamic histomorphometry, undecalcified femora were embedded in acrylic and 50 µm sections were cut by using Isomet-Slow Speed Bone Cutter (Buehler, Lake Bluff, IL) followed by photography using a confocal microscope (LSM 510 Meta, Carl Zeiss, Inc., Thornwood, NY). We enumerated periosteal perimeters, single-labeled surface (sLS), double-labeled surface (dLS) and interlabelled thickness (IrLTh), and these values were employed to calculate mineralizing surface/bone surface (MS/BS), mineral apposition rate (MAR), and bone formation rate (BFR) [40 (link)].

Tripathi A.K., Rai D., Kothari P., Kushwaha P., Sashidhara K.V, & Trivedi R. (2022). Benzofuran pyran hybrid prevents glucocorticoid induced osteoporosis in mice via modulation of canonical Wnt/β-catenin signaling. Apoptosis, 27(1-2), 90-111.

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