Nis elements br image processing and analysis software

The influence of genistein on isoflurane-induced neuronal apoptosis was determined by TUNEL assay as described by Li et al. [5 (link)]. The brain tissues were cut into sections at a thickness of 5 µm, and apoptosis was assessed using the Dead End™ fluorometric TUNEL system kit (Promega, Madison, WI, USA) according to the manufacturer's instructions. The numbers of TUNEL-positive cells in the hippocampal CA1, CA3, and dentate gyrus sections were determined and analyzed using NIS-Elements BR image processing and analysis software (Nikon Corporation, Tokyo, Japan). The concentration of TUNEL-positive cells in each region is presented as the number of TUNEL-positive cells/mm².

Brain tissue from each rat was fixed in 4% paraformaldehyde, followed by embedding in paraffin wax and tissue sections were cut at 5 mm thickness onto glass slides. The Dead End™ Fluorometric TUNEL System kit (Promega Corp., Madison, WI, USA) was used to perform the TUNEL fluorescence assay, according to the manufacturer’s instructions. Slides stored in the dark before the cell nuclei were stained using Hoechst fluorescence stain (Thermofisher Scientific, Waltham, MA, USA). NIS-Elements BR image processing and analysis software (Nikon, Tokyo, Japan) were used to quantify the TUNEL-positive cells in the hippocampal region.