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2 protocols using ah7614

1

Lipid Compound Characterization and Cell Signaling

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PAME, palmitic acid (PA), and stearic acid methyl ester (SAME) were purchased from Sigma-Aldrich (St. Louis, MO) and dissolved in 100% methanol. DC260126, dexamethasone, L-ascorbate-2-phosphate, indomethacin, 1,2-bis (2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetra (acetoxymethyl ester) (BAPTA-AM), triethyl phosphate, and Oil Red O were purchased from Sigma-Aldrich. GW1100 was purchased from Cayman Chemical Company (Ann Arbor, MI), AH7614 was purchased from Abcam (Cambridge, England), U73122 was purchased from Millipore (Burlington, MA), and U0126 was purchased from Calbiochem (San Diego, CA).
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2

Comprehensive Metabolic Profiling Protocol

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The following chemicals were purchased: LA (L0124, Tokyo Chemical Industry, Tokyo, Japan), trilinolein (T1388, Tokyo Chemical Industry), oleic acid (25630-64, Nacalai Tesque, Kyoto, Japan), α-linolenic acid (122-05831, FUJIFILM Wako Pure Chemical Corp., Osaka, Japan), streptozocin (195-15154, FUJIFILM Wako Pure Chemical Corp), olive oil (7121704X1407, Yoshida Pharmaceutical Company, Tokyo, Japan), [14C]-Labelled methyl-α-D-glucopyranoside ([14C] α-MDG; 50 μCi; 1.85 MBq; 250 mCi/mmol) (NEC659V, Perkin Elmer, Waltham, MA, United States), phlorizin (167-24401, FUJIFILM Wako Pure Chemical Corp.), TAK-875 (HY-10480, MedChemExpress, Monmouth Junction, NJ, United States), TUG-891 (HY-100881, MedChemExpress), and AH7614 (ab146181, Abcam, Cambridge, United Kingdom).
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