Human hepatocellular carcinoma cell lines Huh7 (Catalog No. CL-0120) and HepG2 (Catalog No. SCSP-510) were purchased from the National Collection of Authenticated Cell Cultures (Shanghai, China). The normal liver cell line LO2 (Catalog No. CL-0111) was purchased from Procell Life Science & Technology Co., Ltd (Wuhan, China).
Huh7 and HepG2 were cultivated in dulbecco's modified eagle medium (DMEM) supplemented with 10% fetal bovine serum (Catalog No. SH30406.05, Cytiva) and 1% penicillin/streptomycin solution (Catalog No. BL505A, Biosharp), in a 37°C incubator supplemented with 5% CO2. LO2 was cultivated in RPMI1640 supplemented with 10% fetal bovine serum (Catalog No. SH30406.05, CytivaGibco) and 1% penicillin/streptomycin solution (Catalog No. BL505A, Biosharp).
Huh7 and HepG2 were cultivated in dulbecco's modified eagle medium (DMEM) supplemented with 10% fetal bovine serum (Catalog No. SH30406.05, Cytiva) and 1% penicillin/streptomycin solution (Catalog No. BL505A, Biosharp), in a 37°C incubator supplemented with 5% CO2. LO2 was cultivated in RPMI1640 supplemented with 10% fetal bovine serum (Catalog No. SH30406.05, CytivaGibco) and 1% penicillin/streptomycin solution (Catalog No. BL505A, Biosharp).