Saxsquant software

Manufactured by Anton Paar

Sourced in United States, Austria

SAXSquant software is a core application for the analysis of small-angle X-ray scattering (SAXS) data. It provides a comprehensive set of tools for data reduction, modeling, and interpretation.

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Lab products found in correlation

Saxsess camera, by Anton Paar (5 mentions) Kratky type instrument, by Anton Paar (2 mentions) Saxsess system, by Anton Paar (1 mentions) 1400 transmission electron microscope, by JEOL (1 mentions) Tcs 120, by Anton Paar (1 mentions) Pw3830 x ray generator, by Malvern Panalytical (1 mentions) Cyclone reader, by PerkinElmer (1 mentions) Saxsess, by Anton Paar (1 mentions) Ccd camera, by Teledyne (1 mentions) Id 3003 laboratory x ray generator, by GE Healthcare (1 mentions)

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SAXSQuant

10 protocols using saxsquant software

SAXS Characterization of SufU Proteins

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SaSufU and BsSufU were diluted to ∼25 mg/mL
in 50 mM Tris at pH 7.8, 500 mM KCl, and 10% glycerol. SAXS measurements
were made using a PANalytical X-ray generator with a Cu target (at
50 mA, 40 kV) and an Anton-Paar SAXSess system, which is an updated
model of the Kratky camera.⁴⁰ The signal
was acquired with an electronic CMOS detector over a scattering angle
range (2θ) from 0.14 to 10°. Data processing was performed
with SAXSquant software from Anton-Paar and consisted of desmearing
to correct for the line slit geometry of the Kratky method.⁴¹

Hudspeth J.D., Boncella A.E., Sabo E.T., Andrews T., Boyd J.M, & Morrison C.N. (2022). Structural and Biochemical Characterization of Staphylococcus aureus Cysteine Desulfurase Complex SufSU. ACS Omega, 7(48), 44124-44133.

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Transmission Electron Microscopy and SAXS Analysis of Digested Samples

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A drop of each digested or undigested sample was placed on a formvar carbon-coated 300 mesh grid for 20s for adsorption. Excess fluid was wicked off using a filter paper before grids were air-dried. All grids were examined with a JEOL 1400 transmission electron microscope (JEOL, Peabody MA, USA) operated at 120 kV and supplied with a GATAN Orius 1000 camera (GATAN Inc., Pleasanton CA, USA).
Small-angle X-ray scattering (SAXS) SAXS measurements were conducted in a flow-through capillary with a Kratky-type instrument (SAXSess from Anton Paar AG, Graz, Austria) at 21±1 °C. The SAXSess has a low sample-detector-distance of 0.309 m which is appropriate for the investigation of dispersions with low scattering intensities. The experiments were performed with 120 measurement cycles (each averaged over 10 s). The measurements were backgroundcorrected with the respective mixture of aqueous BSA solution or digestive juices without addition of aluminum species. Deconvolution (slit length desmearing) of the SAXS curves was performed with the SAXS-Quant software (Anton Paar AG). Samples analyzed with SAXS were used as prepared. Curve fitting was performed with the software McSAS (Monte Carlo method, version 1.0.1). This procedure was described before 27 .

Sieg H., Kästner C., Krause B., Meyer T., Burel A., Böhmert L., Lichtenstein D., Jungnickel H., Tentschert J., Laux P., Braeuning A., Estrela-Lopis I., Gauffre F., Fessard V., Meijer J., Luch A., Thünemann A.F, & Lampen A. (2017). Impact of an Artificial Digestion Procedure on Aluminum-Containing Nanomaterials. Langmuir : the ACS journal of surfaces and colloids, 33(40).

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SAXS Data Acquisition Protocol

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SAXSess camera (Anton Paar Co., Ltd., Graz, Austria) in a line-collimation system. A semitransparent beam stop was used to attenuate the beam. The samples were placed in a vacuum-proof metallic cell between Mylar windows, and the cell was tightened at both ends. Each sample was exposed to a radiation of λ＝0.154 nm at 25℃ for 20 min in vacuum, and the scattering pattern was detected with a 2D imaging plate detection system. A cyclone reader (Perki-nElmer Inc., MA, USA) was used to read the scattering patterns that were converted to 1D profiles using SAXSquant software (Anton Paar Co., Ltd.) . All data were normalized to the same incident primary beam intensity 22) .

Kafle A., Misono T., Bhadani A., Akamatsu M., Sakai K., Kaise C., Kaneko T, & Sakai H. (2018). Effects of β-Sitosteryl Sulfate on the Hydration Behavior of Dipalmitoylphosphatidylcholine. Journal of oleo science, 67(6).

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SWAXS Characterization of Samples

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SWAXS measurements were performed using a SAXSess camera (Anton Paar, Graz, Austria) attached to a PW3830 sealed-tube anode X-ray generator (PANalytical, Almelo, The Netherlands) operated at 40 kV and 50 mA. A monochromatic X-ray beam of Cu–Kα radiation (λ = 0.1542 nm) with a focused line-shape was generated using a Göbel mirror fitted with a block collimator. The thermostatic sample holder unit (TCS 120, Anton Paar, Graz, Austria) was part of the SAXSess system and provided a temperature accuracy of ±0.1 °C. Two-dimensional (2D) scattering patterns were first recorded on an image plate (IP) detector (Cyclone, Perkin Elmer, Waltham, MA, USA) and were subsequently integrated into 1D scattering intensities, I(q), as a function of the absolute value of the scattering vector, q, using SAXSQuant software (Anton Paar, Graz, Austria).

Aramaki K., Takimoto E, & Yamaguchi T. (2020). Effect of the Cationic Head Group on Cationic Surfactant-Based Surfactant Mediated Gelation (SMG). International Journal of Molecular Sciences, 21(21), 8046.

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SAXS Analysis of Lyotropic Liquid Crystals

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SAXS measurements were performed using a SAXSess camera (Anton Parr, Austria) attached to a PW3830 X-ray generator (PANalytical, The Netherlands) , which was operated at 40 kV and 50 mA, with a sealed-tube anode (Cu-Kα wavelength of 0.154 nm) . The measurements were carried out at room temperature. Two-dimensional scattering patterns recorded on an image plate detector were read out by a Cyclone system (Perkin-Elmer, Downers Grove, IL, USA) and were converted into one-dimensional curves as a function of the scattering vector, q＝ (4π/λ ) sin (θ /2) , where θ is the total scattering angle, with SAXSQuant software (Anton Paar) . All scattering intensities were transmission calibrated by adjusting the attenuated primary intensity at q＝0 to unity and corrected for the background scattering of the sample cell and solvent. The SAXS peak ratio was used to confirm the type of the lyotropic liquid crystals.

Takahashi Y., Shirahata H., Nishimura T., Murai M., Wakita K, & Kondo Y. (2018). Boosting Effect of Amphiphilic Random Copolymers for Bicontinuous Phases. Journal of oleo science, 67(5).

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Thermal and Structural Analysis of Materials

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DSC measurements were performed using a Mettler Toledo DSC 1 (Mettler Toledo, Greifensee, Switzerland) operating with STAR e software under nitrogen atmosphere. The sample weight was adjusted to 5 mg, and samples were placed in an aluminum pan, and then heated from 0 to 80℃ with a heating rate of 3℃/min.
2.4 Small Angle X-ray Scattering (SAXS) and Wide Angle X-ray Scattering (WAXS) SAXS and WAXS measurements (SWAXS measurements) were performed with a SAXSess camera (Anton Paar, Graz, Austria) . Cu-Kα (λ : 1.542 Å) radiation was used. The scattering intensity from each sample was measured at 25℃ for 10 min with a cyclone imaging plate detection system (Perkin-Elmer, Waltham, MA, USA) and the results were analyzed using SAXSQuant software (Anton Paar) .

Uyama M., Araki H., Fukuhara T, & Watanabe K. (2018). Physicochemical Properties of α-Form Hydrated Crystalline Phase of 3-(10-Carboxydecyl)-1,1,1,3,5,5,5-heptamethyl Trisiloxane/Higher alcohol/Polyoxyethylene (5 mol) Glyceryl monostearate/Water System. Journal of oleo science, 67(7).

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SAXS Characterization of Materials

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Scattering experiments were carried out using a W 3830 X-ray generator (PANalytical Co., Ltd., Almedo, Netherlands) and diffraction patterns were recorded using a SAXSess camera (Anton Paar Co., Ltd., Graz, Australia) in a line collimation system. A semitransparent beam stop was available to attenuate the beam. The samples were placed in a vacuum-proof metallic cell between Mylar windows, and the cell was tightened at both ends. Each sample was exposed to radiation of λ＝0.154 nm for 20 minutes in a vacuum, and the scattering pattern was detected on a 2D imaging plate. A cyclone reader (PerkinElmer Inc., Massachusetts, USA) was used to read the scattering patterns, which were converted to 1D profiles using SAXSquant software (Anton Paar Co., Ltd.) . All data were normalized to the same incident primary beam intensity 18, 23) .

Kafle A., Misono T., Bhadani A., Akamatsu M., Sakai K., Kaise C., Kaneko T, & Sakai H. (2018). Effects of β-Sitosteryl Sulfate on the Phase Behavior and Hydration Properties of Distearoylphosphatidylcholine: a Comparison with Dipalmitoylphosphatidylcholine. Journal of oleo science, 67(4).

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Small-Angle X-Ray Scattering Analysis of Cholesteryl-Nonanoate Liquid Crystals

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Small-angle X-ray scattering (SAXS) was performed to investigate the liquid crystalline structure of the cholesteryl-nonanoate-containing dispersions. The measurements were conducted with a SAXSess mc2 system (Anton Paar GmbH, Graz, Austria) using Cu Kα radiation (λ = 0.154) and a CCD detector (measurement range: q = 0 − 6 nm⁻¹). The nanodispersions, as well as hydrogel beads (lipid-containing and placebo), were measured at room temperature in a 1 mm capillary, which was positioned in the beam path at a distance of 309 mm to the CCD detector.
Background and dark current subtraction, as well as desmearing, were performed using the SAXSquant software (Anton Paar GmbH, Graz, Austria). The raw data (dotted lines in Figure 3) were appropriately smoothed (solid lines; Figure 3), and the scattering vector q (nm⁻¹) was determined using OriginLab 2018. The position of the reflections was used to calculate the layer spacing (d) according to Bragg’s law: d = 2π/q.

Knoke S, & Bunjes H. (2021). Transfer Investigations of Lipophilic Drugs from Lipid Nanoemulsions to Lipophilic Acceptors: Contributing Effects of Cholesteryl Esters and Albumin as Acceptor Structures. Pharmaceuticals, 14(9), 865.

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SAXS Characterization of Dispersions

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SAXS measurements were performed in a flow-through capillary with a Kratky-type instrument (SAXSess from Anton Paar, Austria) at 294 1 K. The SAXSess has a low sample-to-detector distance of 0.309 m, which is appropriate for investigation of dispersions with low scattering intensities. The samples were measured as delivered after vortexing for 3 min. The measured intensity was converted to an absolute scale according to Orthaber et al. (2000 ▸ ). The scattering vector magnitude q depends on the wavelength λ of the radiation (λ = 0.154 nm) as q = (4πn/λ)sinθ. Deconvolution (slit length desmearing) of the SAXS curves was performed with the SAXS-Quant software (Anton Paar). Samples analysed with SAXS were used as prepared. Curve fitting was conducted with the software SASfit (Bressler et al., 2015 ▸ ).

Szczerba W., Costo R., Veintemillas-Verdaguer S., Morales M.D, & Thünemann A.F. (2017). SAXS analysis of single- and multi-core iron oxide magnetic nanoparticles. Journal of Applied Crystallography, 50(Pt 2), 481-488.

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Small-Angle X-Ray Scattering Protocol

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Small Angle X-ray Scattering (SAXS) spectra were recorded on a SAXSess mc² apparatus (Anton Paar, Austria), using a line-collimation system. This instrument was associated with a ID 3003 laboratory X-Ray generator (General Electric) equipped with a sealed X-Ray tube (PANalytical, λ Cu Ka = 0.1542 nm) operating at 40 kV and 50 mA. A translucent beam stop allowed the measurement of an attenuated primary beam at q = 0. Sample powders were introduced into a Special glass capillary (1.5 mm, WJM-Glas), before being placed inside an evacuation chamber at 20 °C. The scattering of the X-ray beam was detected by a CCD camera (Princeton Instruments, 309 mm sample-detector distance). Using SAXSQuant software (Anton Paar), 2D images were integrated into 1D scattering intensities I as a function of the magnitude of the scattering vector q (q = (4π/λ) sin θ; where 2θ is the total scattering angle). All data were calibrated by normalizing the attenuated primary intensity, before being corrected for the background scattering from the empty capillary. The scattering data, obtained with a slit collimation, exhibited instrumental smearing. Obtained intensities were then scaled into absolute units using water as the reference material.

Khanji A.N., Michaux F., Petit J., Salameh D., Rizk T., Jasniewski J, & Banon S. (2018). Structure, gelation, and antioxidant properties of curcumin-doped casein micelle powder produced by spray-drying. Food & function, 9(2).

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