Optima formic acid

Manufactured by Thermo Fisher Scientific

Sourced in United States, Czechia

Optima™ formic acid is a high-purity reagent used in various analytical and laboratory applications. It serves as a volatile mobile phase component in liquid chromatography and mass spectrometry techniques.

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Lab products found in correlation

Methanol optima, by Thermo Fisher Scientific (2 mentions) Milli q water system, by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Lobind, by Eppendorf (1 mentions) Metformin hydrochloride, by MP Biomedicals (1 mentions) Milli q plus water purification system, by Merck Group (1 mentions) Ammonium acetate, by Merck Group (1 mentions)

Spelling variants of this product

Formic acid (1906 citations) Optima LC/MS grade formic acid (18 citations) Optima-grade formic acid (9 citations) Formic Acid Optima LC/MS (5 citations) Optima grade 0.1% formic acid (1 citations)

4 protocols using optima formic acid

LC-MS Analysis with High-Purity Reagents

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All LC-MS analyses were collected at the Drug Discovery Platform, Research Institute of McGill University Health Centre. All reagents and solvents were LC-MS grade. OmniSolv^® acetonitrile (ACN) and methanol (MeOH) were sourced from MilliporeSigma (Burlington, MO, USA) and Optima™ formic acid from Fisher (Fisher Chemical™). Ultra-pure deionized water was obtained from the Millipore Milli-Q water system (MilliporeSigma, Burlington, MO, USA).

Facchin C., Fraga-Timiraos A.B., Schmitt J., Babaa N., Pannu N., Aliaga A., Larroque A.L, & Jean-Claude B.J. (2023). Molecular Analysis of the Superior Efficacy of a Dual Epidermal Growth Factor Receptor (EGFR)-DNA-Targeting Combi-Molecule in Comparison with Its Putative Prodrugs 6-Mono-Alkylamino- and 6,6-Dialkylaminoquinazoline in a Human Osteosarcoma Xenograft Model. Cells, 12(6), 914.

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Tissue Sample Preparation for Metabolomics

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At the specified time point, the tissues were removed from the inserts using an 8 mm biopsy punch, placed in centrifuge tubes (Eppendorf Lo-Bind, 2 mL centrifuge tubes), and then flash frozen in liquid N₂. The tubes were then stored at −80 °C until further processing. Then, 1 mL of ice-cold Optima™ methanol (Fisher Scientific) with 0.1% Optima™ formic acid (Fisher Scientific) was added to each tube containing a tissue sample. Keeping the tubes on ice, each sample was ground using a Potter tissue homogenizer for 1–2 min. The samples were then centrifuged for 10 min at 20,000× g at 4 °C. The supernatant was then removed to a glass vial for lipid/metabolite extraction, and the protein pellet was stored at −80 °C for protein extraction.

Dhummakupt E., Jenkins C., Rizzo G., Melka A., Carmany D., Prugh A., Horsmon J., Renner J, & Angelini D. (2022). Proteomic, Metabolomic, and Lipidomic Analyses of Lung Tissue Exposed to Mustard Gas. Metabolites, 12(9), 815.

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Metformin LC-MS Analytical Workflow

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Metformin hydrochloride (Catalog Number:151691, >98.0% purity) was purchased from MP Biomedicals (Solon, OH). 1,1-Dimethyl-d₆-biguanide HCL (Metformin-D6 hydrochloride, CAS:1185166-01-1, 98.5% purity) was purchased from CDN Isotopes (Pointe-Claire, Quebec, Canada). Methanol Optima^™ (Catalog Number: A456), Acetonitrile Optima^™ (Catalog Number: A955), Formic Acid Optima^™ (Catalog Number: A117) and Ammonium Acetate Optima^™ (Catalog Number: A11450) were LC-MS grade and purchased from Fisher Scientific (Pittsburgh, PA). Ultra-pure water was obtained from a Milli-Q Plus water purification system (Millipore, Bedford, MA).

Chaudhari K., Wang J., Xu Y., Winters A., Wang L., Dong X., Cheng E.Y., Liu R, & Yang S.H. (2020). Determination of metformin bio-distribution by LC-MS/MS in mice treated with a clinically relevant paradigm. PLoS ONE, 15(6), e0234571.

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Quantitative Analysis of Vitamin D Metabolites

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Reference standards for vitamin metabolites D₃ (Sigma-Aldrich, Saint Louis, MO, USA), 25(OH)D₂ (Sigma-Aldrich, Saint Louis, MO, USA), 1,25(OH)₂D₃ (Toronto Research Chemicals, Toronto, ON, Canada), 24,25(OH)₂D₃ (Toronto Research Chemicals, Toronto, ON, Canada), 25(OH)D₃ (Toronto Research Chemicals, Toronto, ON, Canada), and 3-epi-25(OH)D₃ (Toronto Research Chemicals, Toronto, ON, Canada) and internal standards 1,25(OH)₂D₃-d₆ (Toronto Research Chemicals, Toronto, ON, Canada), 24,25(OH)₂D₃-d₆ (Toronto Research Chemicals, Toronto, ON, Canada), D₃-d₇ (Toronto Research Chemicals, Toronto, ON, Canada), 3-epi-25(OH)D₃-d₃ (Eurisotop, Gif sur Yvette, France), and 25(OH)D₃-d₆ (Eurisotop, Gif sur Yvette, France) were used. Formic acid Optima, 99% (Fisher Chemical, Pardubice, Czech Republic), zinc sulfate monohydrate, 99% (Merck, Darmstadt, Germany), activated charcoal Norit A (Serva Feinbiochemica, Heidelberg, Germany), ammonium acetate, 96% (Merck, Darmstadt, Germany), ethyl acetate LC-MS (Fluka Analytical, Steinheim, Germany), methanol for LC-MS 99.9% (J.T. Baker, Philipsburg, NJ, USA), and acetonitrile for LC-MS, 99.9% (Honeywell, Seelze, Germany) were used. Deionized water was prepared with a Milli-Q Advantage A10 water treatment system (Millipore, Molsheim, France). Agilent Bond Elut C18 (50 mg, 1 mL) cartridges were used for SPE.

Usoltseva L., Ioutsi V., Panov Y., Antsupova M., Rozhinskaya L., Melnichenko G, & Mokrysheva N. (2023). Serum Vitamin D Metabolites by HPLC-MS/MS Combined with Differential Ion Mobility Spectrometry: Aspects of Sample Preparation without Derivatization. International Journal of Molecular Sciences, 24(9), 8111.

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