Neoscope jcm 5000

Plant samples from whole soil grown plants were detached with a dissecting knife and immediately placed on a 6 mm-wide double adhesive and conductive tape (Canemco Inc., Quebec, Canada) that was pre-attached onto the specimen stage. The specimen was examined with a bench-top scanning electronic microscope (NeoScope JCM-5000, Jeol Ltd, Tokyo, Japan) and images were acquired using the software provided by the manufacturer.

Bulk sediment samples taken by one of us (M.K.) were screen-washed using tap water. Ophiuroid microfossils were picked from dried sieving residues under a dissecting microscope. Selected specimens were cleaned in an ultrasonic bath, mounted on aluminum stubs and gold-coated for scanning electron microscopy (SEM) using a Jeol Neoscope JCM-5000. Skeletal plates of the recent ophiuroid Ophiopholis aculeata used for comparison with the Gotland ophiuroids were extracted from an articulated individual using household bleach, rinsed in tap water and mounted for SEM^{8 (link)}. All figured specimens are deposited in the collections of the Natural History Museum Luxembourg (MnhnL).

The surface layer of each sponge sample was sliced, dehydrated and placed on a microscope sample holder and gold sputtering was done in an argon atmosphere. Adequate care was taken to obtain a homogenous cell gold coating.The surfaces of H. pigmentifera and C. cavernosa were imaged on a Neoscope JCM 5000 scanning electron microscope (JEOL, Japan).

Scanning electron microscopy (SEM) was used to assess the morphology of SMP foams. To perform this analysis, three samples of SMP foams were gold sputter-coated using a 108 Auto Sputter Coater (Cressington Scientific Instruments Ltd, Watford, England) and placed on double-sided carbon tape for imaging in a NeoScope JCM5000 (Jeol USA, Inc., Peabody, MA, USA) scanning electron microscope. Images were taken using an acceleration voltage of 10 kV in High Vacuum mode. Four different regions from each specimen were imaged to identify representative foam morphology. Pore sizes were calculated using the Image J Software (NIH, Bethesda, MD, USA). N = 25 data points were collected for axial and transverse pore size, from samples from each region, for each SMP foam formulation.
Strut thickness of each SMP foam formulation was measured by evaluating the SEM images collected for pore size analysis. Image J software (NIH, Bethesda, MD, USA) was used to measure the width of the struts (n = 5) per formulation.

Polymer characterization
was carried out with NMR spectroscopy (Varian 400 MHz). The microstructures
of hydrogels were investigated with a scanning electron microscopy
(SEM) instrument (JEOL NeoScope JCM-5000, an accelerating voltage
of 10 kV). The rheological properties of gels were evaluated using
a rheometer (Anton PAAR MCR 302). Encapsulation and release of fluorescently
labeled model molecules were analyzed using a Varian Cary 50 Scan
UV/vis spectrophotometer.