Tb0198

Manufactured by Sangon

Sourced in United States

TB0198 is a laboratory equipment that serves the core function of mixing and blending various substances or samples. Its primary purpose is to create homogeneous mixtures through controlled agitation or stirring.

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Lab products found in correlation

7 protocols using tb0198

Immunofluorescence Staining of Cells

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Cells of 0.2 million were seeded in chamber (154526, Thermo Scientific), and cultured for two days. Cells were fixed with cold methanol, membrane perforated with 0.15% Triton X-100 (TB0198, Sangon Biotech), blocked with animal non-immune serum (SP KIT-B, Maxvision), and incubated with primary antibodies overnight at 4℃, and then incubated with secondary antibodies for one hour. Cell nucleus was stained with DAPI (P36931, Life Technologies). Images were captured with confocal microscope (LSM 710, Zeiss) with 100x oil objective lens. The following antibodies were used for immune fluorescence: β-tubulin (1:100, HC101, TransGen Biotech), goat anti-mouse IgG secondary antibody Alexa Fluor 488 (1:200, A-11029, Life Technologies).

Qin Y., Chen W., Liu B., Zhou L., Deng L., Niu W., Bao D., Cheng C., Li D., Liu S, & Niu C. (2017). MiR-200c Inhibits the Tumor Progression of Glioma via Targeting Moesin. Theranostics, 7(6), 1663-1673.

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Immunofluorescence Imaging of Cultured Cells

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Cells of 0.1 million were seeded in chamber (154526, Thermo Scientific, USA) and cultured for 2 days. Cells were fixed with cold methanol, membrane perforated with 0.15% Triton X-100 (TB0198, Sangon Biotech), blocked with animal nonimmune serum (SP KIT-B, Maxvision), incubated with primary antibodies overnight at 4°C, and then incubated with secondary antibodies for 1 hour. Cell nucleus was stained with DAPI (P36931, Life Technologies, USA). Images were captured with confocal microscope (LSM 710, Zeiss, Germany) with 100× oil objective lens. The following antibodies were used for immune fluorescence: goat anti-rabbit IgG secondary antibody Alexa Fluor 546 (1:200, A11035, Invitrogen, USA).

Chen W., Qin Y., Wang D., Zhou L., Liu Y., Chen S., Yin L., Xiao Y., Yao X.H., Yang X., Ma W., Chen W., He X., Zhang L., Yang Q., Bian X., Shao Z.M, & Liu S. (2018). CCL20 triggered by chemotherapy hinders the therapeutic efficacy of breast cancer. PLoS Biology, 16(7), e2005869.

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Immunofluorescence Assay of Breast Cancer Cells

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Breast cancer cells (5000 cells per well) were seeded in chamber (154526, Thermo Scientific) and cultured for 2 days. Cells were fixed with cold methanol, and the membrane was perforated with 0.15% Triton X-100 (TB0198, Sangon Biotech), blocked with animal nonimmune serum (SP KIT-B, Maxvision), and incubated with primary antibodies overnight at 4°C and then with secondary antibodies for 1 hour. Cell nucleus was stained with 4′,6-diamidino-2-phenylindole (P36931, Life Technologies). Images were captured with a confocal microscope (LEICA SP5) with a 63× objective lens. The following antibodies were used for immune fluorescence: MSN (1:100, ab52490, Abcam), NONO (1:100, 11058-1-AP, Proteintech), FLAG (1:100, F7425, Sigma-Aldrich), pPKCζ (1:50, 9378S, CST), and goat anti-rabbit IgG secondary antibody Alexa Fluor 546 (1:200, A-11010, Life Technologies).

Qin Y., Chen W., Jiang G., Zhou L., Yang X., Li H., He X., Wang H.L., Zhou Y.B., Huang S, & Liu S. (2020). Interfering MSN-NONO complex–activated CREB signaling serves as a therapeutic strategy for triple-negative breast cancer. Science Advances, 6(8), eaaw9960.

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Immunofluorescence Staining of Cells

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5 × 10⁴ cells were seeded in a chamber (154526, Thermo Scientific, USA), cultured in a 5% CO₂ 37°C cell incubator for two days, then fixed with cold methanol and treated with 0.15% Triton X100 (TB0198, Sangon Biotech). Animal non-immunized serum (SP KIT-B, Maxvision) was used for blocking. Samples were incubated with primary antibody at 4°C overnight and then incubated with secondary antibody at room temperature for 30 min. Nuclei were stained with DAPI (Invitrogen). Images were captured by confocal microscopy (Leica TCS SP5) with a 63× oil objective.

Ma W., Zhang L., Chen W., Chang Z., Tu J., Qin Y., Yao Y., Dong M., Ding J., Li S., Li F., Deng Q., Yang Y., Feng T., Zhang F., Shao X., He X., Zhang L., Hu G., Liu Q., Jiang Y.Z., Zhu S., Xiao Z., Su D., Liu T, & Liu S. (2024). Microbiota enterotoxigenic Bacteroides fragilis-secreted BFT-1 promotes breast cancer cell stemness and chemoresistance through its functional receptor NOD1. Protein & Cell, 15(6), 419-440.

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Immunofluorescence Staining of Cellular Markers

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Cells were fixed with 4% paraformaldehyde (Dingguo Changsheng Biotechnology) at 37°C for 30 min, then permeabilized with 0.2% Triton X-100 (Shanghai Sangon Biotech, TB0198) at 4°C for 10 min. After blocking in goat serum for 2 hr, cells were incubated with the indicated antibodies for 2 hr at room temperature or overnight at 4°C, washed with 0.05% Triton X-100, and stained with FITC- or CY5-conjugated secondary antibodies (Invitrogen) for 1 hr at room temperature. Cell images were captured with TCS SP5 Leica confocal microscope. The following antibodies were used: CAIX (1:100, Proteintech, 66243-1-Ig); DBC1 (1:1000, CST, 5693); and DAPI (300 nM, Invitrogen, D21490).

Liu Q., Luo Q., Feng J., Zhao Y., Ma B., Cheng H., Zhao T., Lei H., Mu C., Chen L., Meng Y., Zhang J., Long Y., Su J., Chen G., Li Y., Hu G., Liao X., Chen Q, & Zhu Y. (2022). Hypoxia-induced proteasomal degradation of DBC1 by SIAH2 in breast cancer progression. eLife, 11, e81247.

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Immunofluorescence Staining of Cell Organelles

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Cells were fixed with 4% paraformaldehyde (Dingguo Changsheng Biotechnology) at 37°C for 30 min, then permeabilized with 0.2% Triton X-100 (Shanghai Sangon Biotech, TB0198) at 4°C for 10 min. After blocking in goat serum for 2 h, cells were incubated with the indicated antibodies for 2 h at room temperature or overnight at 4°C, washed with 0.05% Triton X-100, and stained with FITC-or CY5-conjugated secondary antibodies (Invitrogen) for 1 h at room temperature. Cell images were captured with TCS SP5 Leica confocal microscope. The following antibodies were used: Myc (1:1000, Santa Cruz, Sc-40); TGN46 (1:500, Abcam, ab41702); Giantin (1:500, Abcam, ab80864); GRASP55 (1:600, Proteintech, 10598-1-AP).

Luo Q., Liu Q., Cheng H., Wang J., Zhao T., Zhang J., Mu C., Meng Y., Chen L., Zhou C., Lei H., Yang J., Chen G., Li Y., Pan L., Chen Q, & Zhu Y. (2022). Nondegradable ubiquitinated ATG9A organizes Golgi integrity and dynamics upon stresses. Cell reports, 40(7).

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Immunofluorescence Staining of Cells

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Cells were fixed with 4% paraformaldehyde (Dingguo Changsheng Biotechnology) at 37°C for 30 min, then stained with the lectin diluent buffer containing 2 μL WGA (5 mg/mL) at 4°C for 30 min. After discarding the staining solution, cells were permeabilized with 0.2% Triton X-100 (Shanghai Sangon Biotech, TB0198) at 4°C for 10 min and blocked by goat serum at room temperature for 2 h. Cells were then incubated with the anti-Biotin antibody overnight at 4°C, washed with 0.05% Triton X-100, and stained with CY5-conjugated secondary antibodies (Invitrogen) for 1 h at room temperature. Cell images were captured with TCS SP5 Leica confocal microscope.

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