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Polyethylene glycol sorbitan monooleate tween 80

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Polyethylene glycol sorbitan monooleate, also known as Tween 80, is a non-ionic surfactant commonly used in laboratory settings. It serves as an emulsifier, dispersing agent, and wetting agent to facilitate the mixing and solubilization of various substances in aqueous and non-aqueous solutions.

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Lab products found in correlation

Chloroauric acid haucl4, by Merck Group (2 mentions) Sorbitan monooleate span 80, by Merck Group (2 mentions) Propylene glycol 400, by Merck Group (1 mentions) Milli q purification system, by Merck Group (1 mentions) Plurol oleique cc 497, by Gattefosse (1 mentions) Castor oil ricinus communis l, by Merck Group (1 mentions) Lauroglycol 90, by Gattefosse (1 mentions) Transcutol p, by Gattefosse (1 mentions) Labrafil m 1944 cs, by Gattefosse (1 mentions) Oleic acid, by Thermo Fisher Scientific (1 mentions) Abietic acid, by Merck Group (1 mentions) Carbopol 940, by Thermo Fisher Scientific (1 mentions) Nisin, by Merck Group (1 mentions) Phosphate buffer saline (pbs), by Thermo Fisher Scientific (1 mentions) Mueller hinton agar m173, by HiMedia (1 mentions) Acetic acid, by Merck Group (1 mentions)

7 protocols using polyethylene glycol sorbitan monooleate tween 80

1

Synthesis of Gold Nanoparticles

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Lavender oil (Lavandula angustifolia) (CAS number 8000-28-0), chloroauric acid (HAuCl4), polyethylene glycol sorbitan monooleate (Tween 80) and sorbitan monooleate (Span 80) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Fadel B.A., Elwakil B.H., Fawzy E.E., Shaaban M.M, & Olama Z.A. (2023). Nanoemulsion of Lavandula angustifolia Essential Oil/Gold Nanoparticles: Antibacterial Effect against Multidrug-Resistant Wound-Causing Bacteria. Molecules, 28(19), 6988.
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2

Apremilast Formulation Development and Characterization

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Apremilast (purity of 99.6% and molecular weight of 460.5 Da) was purchased from Wuhan Senwayer Century Chemical (Wuhan, China). Polyglyceryl-3 dioleate (Plurol® oleique CC497), oleoyl polyoxyl-6 glycerides (Labrafil® M1944 CS), propylene glycol monolaurate-type II (Lauroglycol™ 90), caprylocaproyl polyoxyl-8 glycerides (Labrasol®) and diethylene glycol monoethyl ether (Transcutol® P) were supplied by Gattefossé (Saint-Priest, France). Castor oil (Ricinus communis L.), polyethylene glycol sorbitan monooleate (Tween® 80) and propylene glycol 400 were supplied by Sigma-Aldrich (Madrid, Spain). Macrogol glycerol ricinoleate [Cremophor® EL] was supplied by Fagron Iberica (Barcelona, Spain). Reagents for histological procedures were purchased from Sigma and Thermo Fisher Scientific (Barcelona, Spain). HaCaT cell line was purchased from Cell Line Services (Eppelheim, Germany) and the reagents used for cell cultures were obtained from Gibco (Carcavelos, Portugal). Reagents for MTT assay were obtained from Invitrogen Alfagene® (Carcavelos, Portugal). A Millipore Milli-Q® purification system (Millipore Corporation; Burlington MA) was used to obtain ultrapure water for all experiments. Finally, the reagents used in this study were of analytical grade.
Sarango-Granda P., Silva-Abreu M., Calpena A.C., Halbaut L., Fábrega M.J., Rodríguez-Lagunas M.J., Díaz-Garrido N., Badia J, & Espinoza L.C. (2020). Apremilast Microemulsion as Topical Therapy for Local Inflammation: Design, Characterization and Efficacy Evaluation. Pharmaceuticals, 13(12), 484.
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3

Biocompatible Nanocomposite Film Fabrication

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Silicon dioxide powder SiO2 (size 10–20 nm brand IOLITEC Purity 99%) and Cinnamomum verum essential oil CEO (brand VidaScent purity 99.99%). Ethanol brand CTR Scientific (purity 99.95% density 0.804 g/cm3). Polyethylene glycol sorbitan monooleate Tween 80 (brand Sigma-Aldrich, density 1.07 g/cm3, Oleic acid ≥ 58.0%). Commercial polylactic acid (PLA) filament was used to prepare the films. (brand Inky, white, molecular weight 195,000 g/mol, viscosity 109.76 mPa/s to shear rate between 10–100 s−1, aspect ratio (L/D) approx. 0.5 with an average length of 2000 ± 0.3 µm, an average diameter of 1750 ± 0.2 µm, and a density of 1.24 g/cm3).
Martínez-Aguilar V., Peña-Juárez M.G., Carrillo-Sanchez P.C., López-Zamora L., Delgado-Alvarado E., Gutierrez-Castañeda E.J., Flores-Martínez N.L., Herrera-May A.L, & Gonzalez-Calderon J.A. (2023). Evaluation of the Antioxidant and Antimicrobial Potential of SiO2 Modified with Cinnamon Essential Oil (Cinnamomum Verum) for Its Use as a Nanofiller in Active Packaging PLA Films. Antioxidants, 12(5), 1090.
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4

Synthesis and Characterization of Surfactant Formulations

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Polyethylene glycol sorbitan monooleate (Tween 80, Sigma Aldrich, Saint Louis, MO, USA, ≥99%), oleic acid (Alfa Aesar, Ward Hill, MA, USA, 99%), Carbopol® 940 (Acros Organics, Geel, Belgium), abietic acid (Sigma Aldrich, Saint Louis, MO, USA, ~75%), 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AAPH) and luminol were obtained from Stanchem Sp. z o.o. Przedsiębiorstwo Chemiczne. Reagents were used as received without further purification. The carbamate surfactant CB-16(Bu) was synthesized according to the method described in [38 (link)].
Mirgorodskaya A., Kushnazarova R., Pavlov R., Valeeva F., Lenina O., Bushmeleva K., Kuryashov D., Vyshtakalyuk A., Gaynanova G., Petrov K, & Zakharova L. (2022). Supramolecular Tools to Improve Wound Healing and Antioxidant Properties of Abietic Acid: Biocompatible Microemulsions and Emulgels. Molecules, 27(19), 6447.
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5

Antimicrobial Efficacy of Nisin

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Acetic acid (Merck Co., Germany), nisin (Sigma Aldrich from Lactococcus lactis, potency ≥ 900 IU/mg, purity ≥ 95%, CAS Number 1414-45-5), Brain Heart Infusion (BHI) (BBL 11,407, USA), phosphate buffer saline (PBS) (Oxoid, Basingstoke, UK) were purchased and used as received. Polyethylene glycol sorbitan monooleate (Tween 80) was purchased from Sigma Aldrich. Additionally, Mueller Hinton agar (M173) was purchased from HiMedia (Pvt., India), and LAB204 Neogen Company. While, 0.5 McFarland Standard (8.2 log10 CFU/ml) (Cat. No. TM50) was purchased from Dalynn Biologicals Co. The deionized water was obtained from the Molecular Biology Unit, Assiut University, Egypt.
Elsherif W.M., Hassanien A.A., Zayed G.M, & Kamal S.M. (2024). Natural approach of using nisin and its nanoform as food bio-preservatives against methicillin resistant Staphylococcus aureus and E.coli O157:H7 in yoghurt. BMC Veterinary Research, 20, 192.
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6

Synthesis of Gold Nanoparticles

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Lavender oil (Lavandula angustifolia) (CAS number 8000-28-0), chloroauric acid (HAuCl4), polyethylene glycol sorbitan monooleate (Tween 80) and sorbitan monooleate (Span 80) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Fadel B.A., Elwakil B.H., Fawzy E.E., Shaaban M.M, & Olama Z.A. (2023). Nanoemulsion of Lavandula angustifolia Essential Oil/Gold Nanoparticles: Antibacterial Effect against Multidrug-Resistant Wound-Causing Bacteria. Molecules, 28(19), 6988.
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7

Lipid Nanoparticle Formulation Protocol

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Plasmid pNL1.1CMV (designed by Promega, Madison, WI, USA) was purchased from PlasmidFactory & Co. KG (Bielefeld, Germany). The ionizable aminolipid (6Z,9Z,28Z,31Z)-heptatriacont-6,9,28,31-tetraene-19-yl 4-(dimethylamino)-butanoate (DLin-MC3-DMA) was purchased from DC Chemicals. Helper lipid 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), cholesterol, 2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (PEG-DSPE) and polyethylene glycol sorbitan monooleate (Tween 80) were obtained from Sigma-Aldrich. Poly(ethylene glycol) sorbitan monolaurate (Tween 20) was purchased from Croda International (Snaith, UK).
Zukancic D., Suys E.J., Pilkington E.H., Algarni A., Al-Wassiti H, & Truong N.P. (2020). The Importance of Poly(ethylene glycol) and Lipid Structure in Targeted Gene Delivery to Lymph Nodes by Lipid Nanoparticles. Pharmaceutics, 12(11), 1068.
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