RNA was extrated from untreated and IMB-HDC-treated cells expressing wild-type or mutant STAT5 variants using Trizol reagent (Invitrogen, Waltham, MA, USA). cDNA synthesis was performed with Oligo (dT) or random primers by using a Quantscript RT Kit (Tiangen). Real-time PCR was performed with SsoAdvanced SYBR GreenSupermix (Bio-Rad) and primers for Bcl-2 [10 (link)] and c-myc (forward 5′-AACAGGAACTATGACCTCG-3′, and reverse 5′-AGCAGCTCGAATTTCTTC-3′), CyclinD1 (forward 5′-ACAAACAGATCATCCGCAAACAC-3′ and reverse 5′- TGTTGGGGCTCCTCAGGTTC-3′), Pim-1 (forward 5′-TCTTCTGGCAGGTGCTG-3′ and reverse 5′-GGTAGCGAATCCACTCTG-3′), Osm (forward 5′-AGATACCTGAGCCCACACAGACAG-3′ and reverse 5′-ATCGTCCCATTCCCTGAAGACC-3′), and c-Fos (forward 5′-CGAAGGGAACGGAATAAGATGG-3′ and reverse 5′-AGACCTCCAGTCAAATCCAGGG-3′). Primers for target gene expression of STAT5 were normalized to GAPDH.
Oligo
Manufactured by Tiangen Biotech
Sourced in China
Oligo is a laboratory equipment used for the synthesis of oligonucleotides, which are short sequences of nucleic acids. The core function of Oligo is to automate the process of synthesizing custom DNA or RNA oligonucleotides, allowing researchers to quickly and efficiently produce the desired genetic sequences for various applications.
Automatically generated - may contain errors
2 protocols using oligo
1
Quantitative RT-PCR Analysis of STAT5 Target Genes
2
Chemical Reagents for RNA Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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In our study, chemicals and reagents used were of analytical grade and purchased from Sigma-Aldrich (St. Louis, MO, USA). In addition, Oligo (dT), dNTPs, and M-MLV reverse transcriptase were collected from Tiangen (Beijing, China).
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