Placenta samples were previously mineralized by wet method in a sand bath (J.R. Selecta, Barcelona, Spain); they were placed in a resistant flask and dissolved using nitric acid followed by a mixture of HNO3:HClO4 (69%:70%, v/v; Merck KGaA, Darmstadt, Germany; ratio 1:4, v/v) until complete elimination of organic matter. Fe, Mn, Se, Ba, Cu, Zn were determined using an Agilent 8800 ICP-MS (Agilent, Santa Clara, CA, USA). All ICP-MS standards were prepared from ICP single element standard solutions (Merck KGaA) after appropriate dilution with 10% HNO3. For calibration, two sets of multielement standards containing all the analytes of interest at five levels concentrations were prepared using Rhodium as internal standard.
Agilent 8800 icp ms
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 8800 ICP-MS is an Inductively Coupled Plasma Mass Spectrometer. It is designed to perform highly sensitive, multi-element analysis of a wide range of sample types.
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Lab products found in correlation
Icp single element standard solutions, by Merck Group (2 mentions)
Ultraclave microwave digestion system, by Milestone (2 mentions)
Qubit 2.0 fluorometer, by Thermo Fisher Scientific (2 mentions)
Hclo4, by Merck Group (1 mentions)
Zorbax gf 250 column, by Agilent Technologies (1 mentions)
Asx 500 autosampler, by Agilent Technologies (1 mentions)
Agilent 1100 hplc, by Agilent Technologies (1 mentions)
Avance 3 400 mhz nmr, by Bruker (1 mentions)
J w db 624, by Agilent Technologies (1 mentions)
Gc 6890n, by Agilent Technologies (1 mentions)
Gc 7890a, by Agilent Technologies (1 mentions)
Agilent 7900 icp ms, by Agilent Technologies (1 mentions)
10 protocols using agilent 8800 icp ms
1
Placental Elemental Profiling by ICP-MS
2
Placental Elemental Analysis via ICP-MS
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Prior to being placed in a resistant flask and dissolved in nitric acid, placenta samples were previously mineralized by a wet method in a sand bath (J.R. Selecta, Barcelona, Spain) and were then mixed with HNO3:HClO4 (69%:70%, v/v; Merck KGaA, Darmstadt, Germany; ratio 1:4, v/v) until all organic matter was completely removed. The following elements were measured with an Agilent 8800 ICP-MS: Ca, P, and Mg (Agilent, Santa Clara, CA, USA). After the proper dilution with 10% HNO3, all ICP-MS standards were made from ICP single-element standard solutions (Merck KGaA). Using rhodium as the internal standard, two sets of multi-element standards were created for calibration that included all of the relevant analytes at five different concentration levels.
3
Trace Elemental Analysis of Cellular Samples
4
Inductively Coupled Plasma Mass Spectrometry
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ICP-MS was performed by the MS service
of the University of Zurich (UZH). The measurements were conducted
by an inductively coupled plasma triple quadrupole mass spectrometer
equipped with a standard microflow sprayer or an apex-IR nebulizer
(Agilent 8800 ICP-MS).
of the University of Zurich (UZH). The measurements were conducted
by an inductively coupled plasma triple quadrupole mass spectrometer
equipped with a standard microflow sprayer or an apex-IR nebulizer
(Agilent 8800 ICP-MS).
5
Advanced Analytical Techniques for Pharmaceutical Characterization
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The Agilent GC-7890A (Santa Clara, CA, USA) was equipped with FID and FPD for the analysis of major constituents and structurally relevant impurities. The chromatographic columns used were Agilent J&W DB-1 (30 m × 0.32 mm × 1.00 μm) and DB-5 (30 m × 0.25 mm × 0.25 μm). The Agilent GC-6890N (Santa Clara, CA, USA) was equipped with an FID and a G1888-headspace injector for quantifying residual organic solvents. The chromatographic column used was an Agilent J&W DB-624 (30 m × 0.32 mm × 1.80 μm). The Thermo Q Exactive Plus (Waltham, MA, USA) was equipped with a heated electrospray ionization source (HESI) for the mass spectrometric characterization of TnPP.
Bruker AVANCE III 400 MHz NMR (Billerica, MA, USA) was used for characterization and quantification. Data processing was conducted using Bruker NMR software TopSpin 3.1.
Water content was determined using a METTLER TOLEDO C30S Karl Fischer titrator (Greifensee, Switzerland). Inorganic impurities were analyzed and determined using an Agilent 8800 ICP-MS (Tokyo, Japan). The samples were weighed using a METTLER TOLEDO XP205 or UMX2 balance (Greifensee, Switzerland). A Vortex-Genie2 vortex oscillator G560E was used to prepare homogenized process solutions (Scientific Industries, Inc., Bohemia, NY, USA).
Bruker AVANCE III 400 MHz NMR (Billerica, MA, USA) was used for characterization and quantification. Data processing was conducted using Bruker NMR software TopSpin 3.1.
Water content was determined using a METTLER TOLEDO C30S Karl Fischer titrator (Greifensee, Switzerland). Inorganic impurities were analyzed and determined using an Agilent 8800 ICP-MS (Tokyo, Japan). The samples were weighed using a METTLER TOLEDO XP205 or UMX2 balance (Greifensee, Switzerland). A Vortex-Genie2 vortex oscillator G560E was used to prepare homogenized process solutions (Scientific Industries, Inc., Bohemia, NY, USA).
6
ICP-MS Analysis of 89Zr Solution
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The ICP-MS of three batches of final 89Zr solution to determine metallic impurities were performed and validated by an ISO 9001:2015 certified Institution (Saskatchewan Research Council, Saskatoon SK, country). Niobium and yttrium were analyzed using an Agilent 7900 ICP-MS (Santa Clara, CA, USA) and all other elemental analysis were done using an Agilent 8800 ICP-MS (Santa Clara, CA, USA).
7
Multimodal Analysis of Trace Elements
8
Multimodal Analysis of Trace Elements
9
Quantifying AuNP Translocation by ICP-MS
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ICP-MS analyses were carried out on an Agilent 8800 ICP-MS (Agilent Technologies, Waldbronn, Germany) using an external calibration curve (1000 μg/ml gold standard for ICP; Merck) with internal standardization (1000 μg/ml iridium standard for ICP; Merck). The instrument is equipped with two quadrupole mass analyzers (MS/MS) and a collision/reaction cell. Rinsing was done with 1 % HNO3/2 % HCl between each measurement.
The LOD of the ICP-MS corresponded to a dose of 25 ng/cm2. With lower doses it was not possible to reliably determine the translocated AuNP fraction in the basolateral medium 24 h post-exposure.
The LOD of the ICP-MS corresponded to a dose of 25 ng/cm2. With lower doses it was not possible to reliably determine the translocated AuNP fraction in the basolateral medium 24 h post-exposure.
10
Measurement of Iodine Isotopes in Samples
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All the details of chemical reagents used here are described in the Supplementary Information 2.
The iodine in the sediment was separated by combusting at 800 ℃ for 3 hours in a tube furnace (Catalytic Pyrolyser-4 TrioTM furnace, Raddec LTD, Southampton, UK). The detail analytical method has been reported elsewhere and is summarized in the Supplementary Information 2 (Hou et al., 2010) (link).
The measurement of 129 I/ 127 I atomic ratios was conducted using a 3 MV Tandem AMS system (HVEE) at Xi'an AMS Center, China, and the details of AMS system and measurement method for 129 I have also been reported elsewhere (Hou et al., 2010) (link).The measured 129 I/ 127 I ratios in procedure blank were below 2 × 10 -13 , which was at least 2 orders of magnitude lower than that in samples. The 129 I/ 127 I ratios in the samples were corrected by subtracting the blank. The stable iodine was measured using ICP-MS (Agilent 8800 ICP-MS) at the Xi'an AMS Center. Cs + was added as internal standard, 0.15 mol/L NH3•H2O is used as wash solution between samples. The detection limit of this method is lower than 0.02 ng/L, which is more than 10 times higher than the procedure blank and at least 3 orders of magnitude lower than the samples.
The iodine in the sediment was separated by combusting at 800 ℃ for 3 hours in a tube furnace (Catalytic Pyrolyser-4 TrioTM furnace, Raddec LTD, Southampton, UK). The detail analytical method has been reported elsewhere and is summarized in the Supplementary Information 2 (Hou et al., 2010) (link).
The measurement of 129 I/ 127 I atomic ratios was conducted using a 3 MV Tandem AMS system (HVEE) at Xi'an AMS Center, China, and the details of AMS system and measurement method for 129 I have also been reported elsewhere (Hou et al., 2010) (link).The measured 129 I/ 127 I ratios in procedure blank were below 2 × 10 -13 , which was at least 2 orders of magnitude lower than that in samples. The 129 I/ 127 I ratios in the samples were corrected by subtracting the blank. The stable iodine was measured using ICP-MS (Agilent 8800 ICP-MS) at the Xi'an AMS Center. Cs + was added as internal standard, 0.15 mol/L NH3•H2O is used as wash solution between samples. The detection limit of this method is lower than 0.02 ng/L, which is more than 10 times higher than the procedure blank and at least 3 orders of magnitude lower than the samples.
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