Rats received bilateral infusions into the amygdala. The total volume of the infusion (0.5 µl/side) was given over 60-s, and the injection cannula remained in place an additional 90-s to ensure diffusion away from the injector tip. The injection cannulae were cut to extend approximately 0.5mm beyond the guide cannula. Rats were returned to their home cages after infusions. The specific CaMKII inhibitor myristoylated autocamtide-2 related inhibitory peptide (myr-AIP, 6ng/µl; Enzo Life Sciences) was dissolved in distilled H2O. The myristolated version of this peptide was used to enhance cell permeability. This dosage was determined based on prior work from our lab (Jarome et al., 2013 (link)). Anisomycin (ANI, 125µg/µl; Sigma) was dissolved in HCl and diluted with artificial CSF. A small amount of NaOH was added to bring the pH to ~7.4.
Anisomycin ani
Manufactured by Merck Group
Sourced in United States
Anisomycin (ANI) is a laboratory reagent used for research purposes. It is a protein synthesis inhibitor that functions by blocking the peptidyl transferase center of the 60S ribosomal subunit, thereby preventing the formation of new peptide bonds. This core function makes ANI a valuable tool in the study of cellular processes involving protein synthesis.
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Lab products found in correlation
2 protocols using anisomycin ani
1
Amygdalar CaMKII Inhibition and Anisomycin Infusion
2
Intra-IL Infusion of Muscimol and Anisomycin
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The GABAA receptor agonist muscimol (MUS; Tocris, USA), which suppresses the neurophysiologic activity within 0.5–1.0 mm of the infusion site27 (link), was brought to a final concentration of 0.46 μg/μl using phosphate-buffered saline (PBS) 0.1 M. The protein synthesis inhibitor anisomycin (ANI; Sigma-Aldrich, USA), which inhibits most of de novo protein synthesis for at least 3 h28 (link), was dissolved in PBS 0.1 M to obtain a final concentration of 100 μg/μl. PBS 0.1 M served as the vehicle for both drugs.
On the day of intra-IL infusion, obturators were removed, and the treatment was delivered using two 14 mm dental needles (30G) connected to microsyringes of 5.0 μl by polyethylene tubing (PE10) inserted into the guide cannulae while the animal was gently restrained with a towel. Either 0.2 μl of drug or vehicle solution was given bilaterally for one min using an infusion pump. The needles remained inside guide-cannulae for one min after the end of injections to reduce possible drug backflow.
On the day of intra-IL infusion, obturators were removed, and the treatment was delivered using two 14 mm dental needles (30G) connected to microsyringes of 5.0 μl by polyethylene tubing (PE10) inserted into the guide cannulae while the animal was gently restrained with a towel. Either 0.2 μl of drug or vehicle solution was given bilaterally for one min using an infusion pump. The needles remained inside guide-cannulae for one min after the end of injections to reduce possible drug backflow.
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