Mouse ifn β elisa kit

Bone marrow extracellular fluid was collected as we previously reported [21 (link)]. IFN-β levels in bone marrow extracellular fluid were measured in duplicate using a mouse IFN-β ELISA Kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. The optical density was measured using a Tecan Infinite 200® PRO microplate reader.

Murine IFN-α and IFN-β levels were detected using the Mouse IFN-α ELISA kit and the Mouse IFN-β ELISA kit (both from R&D Systems), respectively. Murine TNF-α and IL-6 were quantified using the ELISA MAX Deluxe Set Mouse IL-6 and ELISA MAX Deluxe Set Mouse TNF-α kits (BioLegend). Human IFN-α was determined with the Human IFN-α Multi-Subtype ELISA Kit (R&D Systems). Proteinuria was examined using the LBIS Mouse Urinary Albumin Assay Kit (Shibayagi). RF-IgM, RF-IgG, and anti-dsDNA antibody levels in serum were measured by ELISA (LBIS Mouse RF-IgM, RF-IgG, and anti-dsDNA antibody kits, Shibayagi). All detection was performed according to the manufacturer’s instructions.

Tumors were established as described above. Mice were randomized (n = 4-6) and intratumorally injected with a single dose of 1 × 10⁷ PFU. At day 4 after virus injection, mice were sacrificed and tumors were harvested, washed with PBS, and fluorescence signals from tumors were acquired using a Geldoc imaging system (Bio-Rad, Hercules, CA) and quantified using ImageJ.
To determine viral titer and IFN-β concentrations within tumors, mice were treated as described above and tumors were harvested, weighted, and homogenized using metal beads and a tissue homogenizer (Qiagen, Hilden, Germany). Virus titers were determined by plaque assays on MA104 cells and IFN-β was quantified using a mouse IFN-β ELISA kit (R&D Systems, Minneapolis, MN, USA). Six mice per group were used to determine IFN-β concentration.