Anti dnmt1 antibody

Manufactured by Active Motif

The Anti-DNMT1 antibody is a laboratory reagent designed for the detection and study of the DNA methyltransferase 1 (DNMT1) protein. DNMT1 is a key enzyme involved in the maintenance of DNA methylation patterns during cell division. This antibody can be used in various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to identify and quantify the DNMT1 protein in biological samples.

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Lab products found in correlation

Streptavidin beads, by Thermo Fisher Scientific (1 mentions) Amersham ecl plus western blotting detection system, by GE Healthcare (1 mentions) Amersham hyperfilm ecl, by GE Healthcare (1 mentions) Mammalian cell lysis kit, by Merck Group (1 mentions) Anti rarα antibody, by Cell Signaling Technology (1 mentions) Amersham ecl, by Cytiva (1 mentions)

Spelling variants of this product

DNMT1 (6 citations) Anti-DNMT1 (3 citations)

2 protocols using anti dnmt1 antibody

Immunoprecipitation of DNMT1 from K562 Cells

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K562 cells were lysed with 10 mM Tris-HCl pH 7.5 containing 200 nM NaCl, 5 mM ethylenediaminetetraacetate (EDTA), 0.1% Triton X-100 and protease inhibitors. Extracts (500 μg in 0.5 ml lysis buffer) were incubated for 30 min with 200 nM heat denatured biotinylated aptamers with rotation. Following three washings with PBS cells, aptamer-protein complexes were purified on streptavidin beads (Thermo Fisher Scientific) for 2 h. Beads were washed three times with PBS and bound proteins were recovered by adding Laemmli buffer and then analyzed by immunoblotting with anti-DNMT1 antibody (Active Motif).

Esposito C.L., Autiero I., Sandomenico A., Li H., Bassal M.A., Ibba M.L., Wang D., Rinaldi L., Ummarino S., Gaggi G., Borchiellini M., Swiderski P., Ruvo M., Catuogno S., Ebralidze A.K., Kortylewski M., de Franciscis V, & Di Ruscio A. (2023). Targeted systematic evolution of an RNA platform neutralizing DNMT1 function and controlling DNA methylation. Nature Communications, 14, 99.

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Western Blot Analysis of DNMT1 and RARα

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ADP cells (2 × 10⁵) were placed on laminin-ornithine coated 35-mm dishes or six-well plates in medium with 20 ng/mL bFGF. We prepared cells 24 hours after cell seeding. Total protein was prepared using the Mammalian Cell Lysis Kit (Sigma). We performed western blotting using anti-DNMT1 antibody (1:1000) (Active Motif, Carlsbad, California) and anti-RARα antibody (1:1000) (Cell Signaling, Danvers, Massachusetts) as described in our previous study (26 (link)). Protein expression was detected using the Amersham ECL Plus Western Blotting Detection System (GE Healthcare, Milwaukee, Wisconsin) and Amersham Hyperfilm ECL (GE Healthcare). Images were converted to digital files and the intensity of bands was analyzed using ImageJ software (National Institutes of Health, Bethesda, Maryland).

Boku S., Toda H., Nakagawa S., Kato A., Inoue T., Koyama T., Hiroi N, & Kusumi I. (2014). Neonatal Maternal Separation Alters the Capacity of Adult Neural Precursor Cells to Differentiate into Neurons Via Methylation of Retinoic Acid Receptor Gene Promoter. Biological psychiatry, 77(4), 335-344.

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