Goat anti mouse igg h l hrp ab6789

Sepasol-RNA I Super G was purchased from Nacalai Tesque (Kyoto, Japan). Isopentane, formaldehyde, 2-mercaptoethanol, and 1% eosin Y solution were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). The PrimeScript RT Reagent Kit with gDNA Eraser was purchased from TaKaRa Bio (Shiga, Japan). SYBR FAST qPCR kits were purchased from Kapa Biosystems (Wilmington, MA, USA). Primary antibodies against AMPKα (#2532), phosphorylated Thr-172 AMPKα (#2535), Akt (#9272), phosphorylated Ser-473 Akt (#4058), forkhead box protein O1 (FOXO1) (#2880), phosphorylated Ser-256 FOXO1 (#9461), FOXO3a (#12829), and phosphorylated Ser-253 FOXO3a (#13129) were purchased from Cell Signaling Technology (Danvers, MA, USA); MEF2A (sc-313), PGC-1α (sc-517380), and Sp1 (sc-14027) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA); and α-tubulin (#017-25031) was obtained from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). For secondary antibodies, goat anti-mouse IgG H&L (HRP) (ab6789) and goat anti-rabbit IgG H&L (HRP) (ab6721) were purchased from Abcam plc. (Cambridge, UK), and goat anti-rabbit IgG-HRP (sc-2004) was purchased from Santa Cruz Biotechnology. Polyvinylidene difluoride membrane (Immobilon-P, 0.45μm) for Western blotting was purchased from Merck (Darmstadt, Germany).

Bio-Rad protein assay dye reagent [50 (link)], iProof DNA polymerase, Nuvia^TM IMAC resin, Precision plus protein dual color standards, and reagents for protein electrophoresis were mainly purchased from Bio-Rad, Hercules, CA, USA. Restriction endonucleases, PrimeSTAR DNA polymerase, and the In-Fusion HD cloning kit were obtained from Takara, Japan. SeaKem^® LE Agarose was purchased from Lonza, Morristown, NJ, USA. The Presto^TM Mini Plasmid kit and GenepHlow^TM Gel/PCR kit were supplied by Geneaid, New Taipei City, Taiwan. Goat anti-mouse IgG H&L (HRP, ab6789) and a colorimetric phosphate assay kit—PiColorLock^TM (ab27004) were obtained from Abcam, Cambridge, UK. Immobilon PVDF membranes, Western Chemiluminescent HRP substrate (ECL), and anti-6x His tag monoclonal antibodies (Cat. # 05-949, Lot # 2869961) were purchased from Merck Millipore, Burlington, MA, USA. Di-C18:0 PA (Lot # 830865P-25MG-C-045) was obtained from Avanti Polar Lipids, Alabaster, AL, USA. Oligonucleotide synthesis and DNA sequencing were carried out by Tri-I Biotech, New Taipei City, Taiwan. All other chemicals were reagent grade.