Liquiritigenin

Manufactured by Bio-Techne

Sourced in United States

Liquiritigenin is a natural compound isolated from the root of the licorice plant (Glycyrrhiza glabra). It is a flavonoid compound with potential biological activities. The core function of Liquiritigenin is as a chemical standard for research and analytical applications.

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Lab products found in correlation

3 protocols using liquiritigenin

Regulation of Breast Cancer Cell Lines

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Parental and ERβ1-expressing MCF7 cells [12 (link)] and doxycycline-inducible Hs578T-ERβ1 cells [8 (link)] were cultured as previously described. Doxycycline-inducible ERβ1-expressing MDA-MB-231 cell lines were established using the T-REx™ System (Invitrogen) as previously described [9 (link)] and were maintained in DMEM/F12 medium supplemented with 10% FBS, 1% AA, 5 mg/L blasticidin S and 500 mg/L zeocin. Charcoal-stripped fetal bovine serum (CS-FBS) was purchased from Gemini Bio-Products (West Sacramento, CA). 17β-estradiol (E2), (Z)-tamoxifen, (Z)-4-hydroxy-tamoxifen and doxycycline (Dox) were purchased from Sigma-Aldrich (St. Louis, MO). (Z)-endoxifen was synthesized by the National Cancer Institute (Bethesda, MD). The ERβ-specific agonists; DPN, WAY200070, FERb 033 and Liquiritigenin, as well as the pure ER antagonist ICI 182,780, were purchased from Tocris Bioscience (Bristol, United Kingdom).

Reese J.M., Suman V.J., Subramaniam M., Wu X., Negron V., Gingery A., Pitel K.S., Shah S.S., Cunliffe H.E., McCullough A.E., Pockaj B.A., Couch F.J., Olson J.E., Reynolds C., Lingle W.L., Spelsberg T.C., Goetz M.P., Ingle J.N, & Hawse J.R. (2014). ERβ1: characterization, prognosis, and evaluation of treatment strategies in ERα-positive and -negative breast cancer. BMC Cancer, 14, 749.

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Quantitative Analysis of Flavonoids

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All solvents used for solid‐phase extraction, HPLC analysis/purification and MS‐based experiments were LC‐MS grade from either Sigma‐Aldrich or VWR Chemicals. Authentic standards for LC‐HRESIMS quantification and molecule identification through LC‐HRESIMS and LC‐UV/vis were purchased from different suppliers: p‐coumaric acid (Sigma Aldrich, USA), naringenin (Sigma Aldrich, USA), dihydro kaempferol (Sigma Aldrich, USA), kaempferol (Cayman Chemical, USA), apigenin (Extrasynthese, Genay, France), 2‐hydroxynaringenin (Ambinter, Orléans, France), iso liquiritigenin (Sigma Aldrich, USA), liquiritigenin (Tocris Bioscience, Bristol, UK), (2R,3R)‐garbanzol (BioBioPha, Kunming, Yunnan, China), (2S,3S)‐garbanzol (AnalytiCon Discovery, Potsdam, Germany), resokaempferol (Extrasynthese), fustin (Biosynth Carbosynth, Compton, UK), butin (ChemFaces, Wuhan, China), 7,4′‐dihydroxyflavone (Extrasynthese, France), quercetin (Cayman Chemical) and dihydroquercetin (Sigma Aldrich).

Marín L., Gutiérrez‐del‐Río I., Villar C.J, & Lombó F. (2021). De novo biosynthesis of garbanzol and fustin in Streptomyces albus based on a potential flavanone 3‐hydroxylase with 2‐hydroxylase side activity. Microbial Biotechnology, 14(5), 2009-2024.

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Estrogen receptor signaling in breast and liver cells

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Estradiol was from Sigma (St Louis, MO). Liquiritigenin and iso-Liquiritigenin, and the antiestrogen ICI 182,780 were from Tocris Bioscience (Bristol, UK). All compounds were checked for identity and purity by mass spectrometry and NMR. MCF-7 cells (ATCC) were cultured in DMEM, Dulbecco’s Minimal Essential Medium (Gibco/Life Technologies, Grand Island, NY), supplemented with 5% calf serum (HyClone, Logan, UT) and 100 μg/ml penicillin/streptomycin (Invitrogen, Carlsbad, CA), as previously described [7 (link), 8 (link)]. For estrogen-free experiments, the cells were seeded in phenol red-free DMEM (Gibco/Life Technologies) plus 5% charcoal-dextran-treated calf serum for 3 days before treatments with compounds were initiated. HepG2 cells (ATCC) and HepG2 cells stably expressing ERα (HepG2+ERα cells, kindly provided by David J. Shapiro, University of Illinois at Urbana-Champaign) were grown as described [9 (link), 10 (link)]. The siRNA experiments for knockdown of the endogenous ERα in MCF-7 cells were performed as previously described and resulted in knockdown of ERα mRNA and protein by greater than 95% [11 (link)]. siERα sequences (Dharmacon, Lafayette, CO) were forward, 5′-UCAUCGCAUUCCUUGCAAAdTdT-3′, and reverse, 5′-UUUGCAAGGAAUGCGAUGAdTdT-3′ [11 (link)].

Boonmuen N., Gong P., Ali Z., Chittiboyina A.G., Khan I., Doerge D.R., Helferich W.G., Carlson K.E., Martin T., Piyachaturawat P., Katzenellenbogen J.A, & Katzenellenbogen B.S. (2015). Licorice Root Components in Dietary Supplements are Selective Estrogen Receptor Modulators with a Spectrum of Estrogenic and Anti-Estrogenic Activities. Steroids, 105, 42-49.

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