We measured glucose levels (mmol/L) using the glucose hexokinase method (Roche Diagnostics, Indianapolis, US) at the CHUS biochemistry clinical laboratory rapidly after blood collection [18 (link), 19 (link)]. We calculated the AUCglu based on the trapezoidal formula [20 (link)], to capture a global measure of maternal hyperglycemia in pregnancy that encompasses all three time-points of the OGTT since all of them have been associated with adverse pregnancy outcomes [5 ], and the AUCglu was the most informative phenotype in prior EWAS investigating dysregulation in child cord blood DNAm [15 (link)].
Glucose hexokinase method
Manufactured by Roche
Sourced in United States, Switzerland
The glucose hexokinase method is a laboratory technique used to measure the concentration of glucose in a sample. It utilizes the enzymatic reaction of glucose with the enzyme hexokinase to produce glucose-6-phosphate, which is then measured to determine the original glucose content.
Automatically generated - may contain errors
Lab products found in correlation
7 protocols using glucose hexokinase method
1
Maternal Glucose Metabolism and Pregnancy Outcomes
2
Blood Sample Processing and Coagulation Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All blood samples were centrifuged within 1 h at 1500 g at 4 °C for 10 min, plasma was separated (separated plasma of citrate samples was centrifuged again for 10 min) and stored immediately at − 70 °C. Plasma glucose concentrations were measured with a glucose hexokinase method (Roche/Hitachi, Indianapolis, USA). D-dimer, factor VIII activity and AT were measured on an automated coagulation analyser (Siemens BCS-XP system) using protocols and reagents from the manufacturer (Siemens Healthcare Diagnostics, Marburg, Germany). Antigen levels of vWF were assayed by ELISA using antigens from DAKO (Heverlee, Belgium). F1 + 2 and TAT were determined by ELISA from Siemens Healthcare Diagnostics, PAI-1 was determined by ELISA from BioMed and PAP was determined by ELISA from DRG diagnostica (Marburg, Germany).
3
Fetal Glycemic Control Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Insulin and C-peptide cord-blood levels were used as a surrogate measure of glycemic control during pregnancy as suggested by the HAPO study.[11 (link)] Maternal blood at 2nd trimester and cord blood were stored at –80°C until analysis. Insulin and C-peptide concentrations were quantified in maternal blood at 2nd trimester and in cord blood using multiplexed particle-based flow cytometric assays (Luminex technology, Millipore Corp, Billerica, MA, USA). Maternal plasma glucose was quantified using the glucose hexokinase method (Roche Diagnostics, Indianapolis, IN, USA). Intra- and inter-assays coefficients of variation are 1.0% and <1.7% for glucose and < 10% and <15% for C-peptide and insulin, respectively.
4
Fasting Glucose and HbA1c Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fasting blood glucose (FBG) and HbA1c were measured as recommended by the national guidelines. FBG was measured by the glucose-hexokinase method (Roche, Base, Switzerland); HbA1c was measured with a high-performance liquid chromatographer standardized and aligned to the United Kingdom Prospective Diabetes Study (UKPDS) and the Diabetes Chronic Complications Trial (DCCT) (Menarini, Florence, Italy). For this study, we collected data at baseline and after 6 months.
5
Assessing Maternal Metabolic Profile
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In plasma samples collected between 24th and 29th week of pregnancy, we measured glucose and insulin levels using glucose hexokinase method (Roche Diagnostics) and Human Milliplex map kits (EMD Millipore, catalog no HMHEMAG-34K), respectively,14 (link) as well as total cholesterol, high-density lipoprotein cholesterol (HDL-C) and triglyceride levels using colorimetric methods (Johnson & Johnson Clinical Diagnostics). We estimated IS with the Matsuda Index using the formula: 10 000/√((fasting glucose×fasting insulin)×(mean glucose×mean insulin during OGTT)),16 (link) with concentrations in glucose and insulin being expressed as mg/dL and µU/mL.17 (link) We excluded from the analyses participants for which data were missing for the Matsuda Index (n=15). We computed low-density lipoprotein cholesterol (LDL-C) concentration with the Friedewald’s equation.14 (link)
6
Fasting Blood Lipid and Glucose
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood sample was withdrawn after at least 8 h of fasting. Blood lipids and fasting plasma glucose were measured. Lipids were measured with a commercially available kit and fasting plasma glucose with the glucose-hexokinase method (Roche, Basel, Switzerland).
7
Insulin Sensitivity Assessment in Pregnant Women
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We measured glucose by the glucose hexokinase method (Roche Diagnostics) immediately after collection and insulin using multiplexed particle-based flow cytometric assays (Human MILLIPLEX map kits, EMD Millipore) from plasma samples previously frozen (−80°C). We estimated insulin sensitivity using the Matsuda index, given by the following equation: 10,000/ (√[(fasting glucose × fasting insulin) × (mean glucose during OGTT × mean insulin during OGTT)]) (7 (link)). We selected the Matsuda index over other measures of insulin sensitivity because it has been validated against euglycemic-hyperinsulinemic clamps in pregnant women (7 (link)).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!