Young tobacco leaves were accurately weighed (0.1 g) and fully ground in liquid nitrogen, following the strict operating instructions of the RNAprep Pure Plant Plus Kit (Polysaccharides Polyphenolics-rich, DP441, TIAN GEN, Beijing, China). The purity and concentration of RNA were determined using a microspectrophotometer. Qualified RNA was used for cDNA synthesis, which was performed strictly according to the instructions for use of the RNA PCR Kit v2.1 (TaKaRa, Dalian, China), employing AMV Reverse Transcriptase XL as the enzyme. Finally, the concentration and purity of cDNA were determined using a trace spectrophotometer.
Rna pcr kit v2
Manufactured by Takara Bio
Sourced in China
The RNA PCR Kit v2.1 is a laboratory equipment product designed for the reverse transcription and amplification of RNA samples. It provides the necessary reagents and protocols for efficient RNA-to-cDNA conversion and subsequent polymerase chain reaction (PCR) analysis.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using rna pcr kit v2
1
Accurate RNA Extraction from Tobacco Leaves
2
Accurate RNA Extraction from Tobacco Leaves
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Young tobacco leaves were accurately weighed (0.1 g) and fully ground in liquid nitrogen, following the strict operating instructions of the RNAprep Pure Plant Plus Kit (Polysaccharides Polyphenolics-rich, DP441, TIAN GEN, Beijing, China). The purity and concentration of RNA were determined using a microspectrophotometer. Qualified RNA was used for cDNA synthesis, which was performed strictly according to the instructions for use of the RNA PCR Kit v2.1 (TaKaRa, Dalian, China), employing AMV Reverse Transcriptase XL as the enzyme. Finally, the concentration and purity of cDNA were determined using a trace spectrophotometer.
3
Accurate RNA Extraction from Tobacco Leaves
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Young tobacco leaves were accurately weighed (0.1 g) and fully ground in liquid nitrogen, following the strict operating instructions of the RNAprep Pure Plant Plus Kit (Polysaccharides Polyphenolics-rich, DP441, TIAN GEN, Beijing, China). The purity and concentration of RNA were determined using a microspectrophotometer. Qualified RNA was used for cDNA synthesis, which was performed strictly according to the instructions for use of the RNA PCR Kit v2.1 (TaKaRa, Dalian, China), employing AMV Reverse Transcriptase XL as the enzyme. Finally, the concentration and purity of cDNA were determined using a trace spectrophotometer.
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