Canto instrument

Manufactured by BD

Sourced in United States

The Canto instrument is a flow cytometry system designed for the analysis of cells and particles. It is capable of detecting and measuring multiple parameters of individual cells or particles in a sample. The Canto instrument provides accurate and reliable data to support research and clinical applications.

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Lab products found in correlation

Collagenase type 2, by Merck Group (1 mentions)

Close spelling variants of this product

FACS Canto 10-color instruments Canto BD II flow cytometer instrument BD Canto II instrument BD FACS Canto II flow cytometer instrument FACS Canto II instrument

2 protocols using canto instrument

Cell Cycle Analysis by Flow Cytometry

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For cell-cycle analysis, cells were fixed in 70% ethanol for at least 1 h at 4 °C. Then cells were washed and resuspended in 1 ml PBS containing 8 μg RNAse A and 50 μg propidium iodide. The cell suspensions were incubated in the dark at room temperature for 30 min prior to FACS analysis. Flow cytometry was performed on a Becton Dickinson Canto instrument (BD Biosciences, San Jose, CA, USA) and red fluorescence PI-labeled cells were measured through a 600 nm wave-length filter. Analysis was performed according to the manufacturer's instructions.

Li J., Liu J., Liang Z., He F., Yang L., Li P., Jiang Y., Wang B., Zhou C., Wang Y., Ren Y., Yang J., Zhang J., Luo Z., Vaziri C, & Liu P. (2017). Simvastatin and Atorvastatin inhibit DNA replication licensing factor MCM7 and effectively suppress RB-deficient tumors growth. Cell Death & Disease, 8(3), e2673-.

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Stromal Vascular Fraction Analysis of Epididymal Adipose Tissue

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Epididymal adipose tissue was excised from 26w old HFD mice, minced, and
incubated in 1mg/ml Type 2 Collagenase (Sigma) for 1 hour at 37°C.
Folllowing RBC lysis, the stromal vascular fraction cells were counted and
stained. Data were acquired on a Canto instrument (BD Biosciences) equipped for
the detection of 8 fluorescent parameters. The following antibodies were used
for FACS analysis: anti-CD3-PerCPCy5.5 (145-2C11), anti-CD11b eFluor450 (M1/70),
anti-B220-APC (RA3-6B2), anti-CD45-PECy7 30-F11) (all from eBioscience);
anti-CD19-PECy7 (1D3), anti-GR1-APCCy7 (RB6-8C5), (all from BD Biosciences);
anti-F4/80-PerCPCy5.5 (BM8.1) (Tonbo Biosciences).

Skeldon A.M., Morizot A., Douglas T., Santoro N., Kursawe R., Kozlitina J., Caprio S., Mehal W.Z, & Saleh M. (2015). Caspase-12, but not caspase-11, inhibits obesity and insulin resistance. Journal of immunology (Baltimore, Md. : 1950), 196(1), 437-447.

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