Hek 293t

HEK-293T and HEK-293T-ACE2 cell lines were obtained from Tsingke Biotechnology Co., Ltd. (Hangzhou, China). The cells were all cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Invitrogen, USA) containing 10% fetal bovine serum (FBS; Gibco) and 1% penicillin-streptomycin (Gibco) at 37°C with 5% CO₂ supplied. pET21a(+) plasmid, pET21a(+)/Zwt plasmids, and E. coli BL21(DE3) strain were stocks in our laboratory. We also used E. coli TG1 strain, pCANTAB5E phagemid vector, and mouse anti-M13K MAb from the Bio-Viewshine company (Beijing, China) and restriction endonucleases (NdeI and XhoI) and T4 DNA ligase (New England BioLabs, MA). Isopropyl-d-thiogalactopyranoside (IPTG), paraformaldehyde, Triton X-100, ampicillin, kanamycin, and defatted milk powder were all products of Sigma (Saint Louis, USA). Ni-NTA agarose was from Qiagen (Dusseldorf, Germany). Bicinchoninic acid (BCA) kit was from Beyotime (Beijing, China). PCR master mix was purchased from Tiangen company (Beijing, China).

HEK-293T and HEK-293T-ACE2 cell lines were obtained from Tsingke Biotechnology Co., Ltd. (Hangzhou, China). The cells were all cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Invitrogen, USA) containing 10% fetal bovine serum (FBS; Gibco) and 1% penicillin-streptomycin (Gibco) at 37°C with 5% CO₂ supplied. pET21a(+) plasmid, pET21a(+)/Zwt plasmids, and E. coli BL21(DE3) strain were stocks in our laboratory. We also used E. coli TG1 strain, pCANTAB5E phagemid vector, and mouse anti-M13K MAb from the Bio-Viewshine company (Beijing, China) and restriction endonucleases (NdeI and XhoI) and T4 DNA ligase (New England BioLabs, MA). Isopropyl-d-thiogalactopyranoside (IPTG), paraformaldehyde, Triton X-100, ampicillin, kanamycin, and defatted milk powder were all products of Sigma (Saint Louis, USA). Ni-NTA agarose was from Qiagen (Dusseldorf, Germany). Bicinchoninic acid (BCA) kit was from Beyotime (Beijing, China). PCR master mix was purchased from Tiangen company (Beijing, China).