Sample solutions were applied to carbon-coated copper grids and negatively stained with 2.0% (w/v) phosphotungstic acid or 2.0% (w/v) uranyl acetate. Images were observed with a JEM-1010 transmission electron microscope (JEOL, Tokyo, Japan) operating at 100 kV using a BIOSCAN model 792 CCD camera, a JEM-1011 transmission electron microscope (JEOL, Tokyo, Japan) operating at 100 kV using a TVIPS TemCam-F114 CCD camera or a TemCam-F415 CCD camera, or a JEM-2010 transmission electron microscope (JEOL, Tokyo, Japan) operating at 200 kV using a Orius SC200D model 833 CCD camera (Gatan, Pleasanton, CA, USA).
Jem 1011 transmission
Manufactured by JEOL
Sourced in Japan
The JEM-1011 is a transmission electron microscope (TEM) manufactured by JEOL. It is designed to provide high-resolution imaging and analysis of a wide range of materials at the nanoscale level. The JEM-1011 features a tungsten filament electron source, a condenser lens system, and an objective lens that allow for the generation and focused targeting of the electron beam onto the sample. This enables the detailed observation and characterization of the internal structure and composition of the specimen under investigation.
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Lab products found in correlation
9 protocols using jem 1011 transmission
1
Transmission Electron Microscopy Imaging
2
Phage vB_EfaH_163 Concentration and Visualization
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Phage vB_EfaH_163 was concentrated using the PEG/NaCl method [32 (link)]. Electron microscopy images were obtained as previously described [33 (link)]: phage particles were stained with 2% uranyl acetate solution, and electron micrographs produced using a CCD Gatan Erlangshen ES 1000 W camera coupled to a JEOL JEM 1011 transmission electron microscope (JEOL USA, Inc., Peabody, MA, USA) operating at 100 kV (performed at the Electron Microscopy Service of the Biotechnology National Centre [CNB-CSIC], Madrid, Spain).
3
Structural Analysis of FliI Ring Complexes
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Purified proteins (final concentration 1 μM) was incubated in 50 mM Tris–HCl, pH 8.0, 113 mM NaCl, 0.8 mM EDTA, 1 mM DTT, 5 mM MgCl2, 5 mM ADP, 5 mM AlCl3, and 15 mM NaF at room temperature for 20 min. Samples were applied to carbon-coated copper grids and negatively stained with 2% (w/v) uranyl acetate. Electron micrographs were recorded at a magnification of ×50,000 with a JEM-1011 transmission electron microscope (JEOL, Tokyo, Japan) operated at 100 kV. To carry out two-dimensional class averaging of the FliI-3A, FliI-4A, and FliI-5A ring structures, 154, 100, and 103 particle images were picked manually, aligned, classified, and averaged using the RELION3.0.7 program45 (link).
4
Ultrastructural Leaf Tissue Analysis
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Leaf tissue blocks were fixed in 2.5% glutaraldehyde, 0.1 M potassium phosphate pH 7.2, washed and postfixed in 1% osmium tetroxide, 0.1 M buffer for 2 hr at 4°C, dehydrated and embedded in Spurr's resin. Sections were cut at 60 nm using a Diatome diamond knife on a Reichert‐Jung Ultracut S ultramicrotome. Sections were picked up on grids and poststained using 4% uranyl acetate and lead citrate. Grids were observed in a JEOL JEM 1,011 transmission electron microscope operated at 80 kV.
5
Transmission Electron Microscopy of Bacterial Cell Morphology
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Selected strains (two adherent and two non-adherent) for each species were examined by TEM after growth at 37°C in Luria broth. Bacteria were applied to Formvar-coated grids and were air dried. The cells were then negatively stained with 1% phosphotungstic acid in distilled water for 5 s and were examined with a JEM-1011 transmission electron microscope (JEOL) operating at 80 kV and equipped with an Orius SC1000 charge-coupled device (CCD) camera (Gatan).
6
Transmission Electron Microscopy of NTHi
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PBS normalized NTHi suspensions (OD600 = 1) were prepared by using NTHi strains freshly grown on chocolate agar. Then, 100 µl of such suspensions were incubated with none or with 100 µl sodium deoxycholate 0.5 mg/ml for 20 min at RT in static conditions. Bacteria were next applied to Formvar-coated grids, air dried, negatively stained with 1% phosphotungstic acid in distilled water for 10 s, and examined with a JEM-1011 transmission electron microscope (JEOL) operating at 80 kV and equipped with an Orius SC1000 charge-coupled device (CCD) camera (Gatan).
7
H. influenzae Ultrastructure Visualization
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H. influenzae strains were examined by TEM after growth on chocolate agar following established procedures (Remuzgo-Martinez et al., 2015 (link)). Briefly, bacteria were applied to Formvar-coated grids, air dried, negatively stained with 1% phosphotungstic acid in distilled water for 10 s, and examined with a JEM-1011 transmission electron microscope (JEOL) operating at 80 kV and equipped with an Orius SC1000 charge-coupled device (CCD) camera (Gatan).
8
Kidney Histological Analysis Protocol
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The kidneys were sectioned and fixed in 10% formalin, dehydrated and embedded in paraffin. Tissues were sectioned at 5 µm and stained with PAS. The kidney tissues were evaluated for a semi-quantitative scoring method by a researcher-blinded to treatments of mice, as explained by Ayla et al. 45) (link) The photographs of sections were taken at different magnifications in a Nikon Eclipse Ni research microscope, Amsterdam, Netherlands, fitted with Nikon DS-Fi2 model digital camera and used Nikon Sight D5 V3 software.
Electron Microscopy After necropsy, the left kidney cortex was immediately divided into pieces that were each 1 mm 3 and prepared for transmission electron microscopy as described by Seckin et al. 46) (link) The samples were investigated by Jeol JEM 1011 transmission electron microscope.
Statistical Analysis The results were expressed as means ± standard error of the mean (S.E.M.). All statistical comparisons were performed by one-way ANOVA followed by Dunnett's tests. p < 0.05 was considered statistically significant.
Electron Microscopy After necropsy, the left kidney cortex was immediately divided into pieces that were each 1 mm 3 and prepared for transmission electron microscopy as described by Seckin et al. 46) (link) The samples were investigated by Jeol JEM 1011 transmission electron microscope.
Statistical Analysis The results were expressed as means ± standard error of the mean (S.E.M.). All statistical comparisons were performed by one-way ANOVA followed by Dunnett's tests. p < 0.05 was considered statistically significant.
9
Nanoparticle-Virus Interaction Visualization
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30 μL of the nanoparticle and virus suspension mixtures, as previously described in cytotoxicity assay part, were incubated at RT for 10 and 60 min and then transferred by pipette to carbon-coated copper TEM grids (400 mesh). After 10 min at RT, the liquid was blotted with filter paper and a droplet of 3 wt% PTA solution was loaded onto the grid for negative staining. The excess PTA solution was blotted with filter paper after 10–40 s, followed by washing 2× with a droplet of deionized water, and the grid was dried for 4 h prior to analysis. TEM images were obtained with a JEM-1011 transmission electron microscope (JEOL Ltd., Tokyo, Japan).
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