Magnesium sulfate heptahydrate

Manufactured by Thermo Fisher Scientific

Sourced in United Kingdom, United States

Magnesium sulfate heptahydrate is a chemical compound with the formula MgSO4·7H2O. It is a naturally occurring mineral salt that is commonly used in various laboratory applications.

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5 protocols using magnesium sulfate heptahydrate

Preparation of Buffers and Materials

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Agarose, lysogeny broth (LB),
poly(ethylene glycol) PEG (Mn 4000), poly(vinyl alcohol) (PVA) (MW
10 000, dialyzed), and poly(vinylpyrrolidone) (PVP) (Mn 40 000)
were purchased from Sigma-Aldrich (Merck). Cesium chloride, magnesium
sulfate heptahydrate, sodium chloride, and PEG (Mn 8000) were purchased
from Fisher Scientific. Glycerol was purchased from Scientific Laboratory
Supplies (SLS). Hydroxyethyl starch (HES) was purchased from Carbosynth.
Phosphate-buffered solution (PBS) (8 g·L^–1 NaCl,
0.2 g·L^–1 KCl, 1.15 g·L^–1 Na₂HPO₄, 0.2 g·L^–1 KH₂PO₄) and Tris-HCl (24.2 g·L^–1 Tris, 80 g·L^–1 NaCl) were prepared by media
preparation facility in the School of Life Sciences at the University
of Warwick. SM-I buffer (1 M NaCl, 8 mM MgSO₄·7H₂O, 22.5 mM Tris-HCl pH 7.5) and SM-II buffer (100 mM NaCl,
8 mM MgSO₄·7H₂O, 22.5 mM Tris-HCl pH 7.5)
was prepared in-house.

Marton H.L., Styles K.M., Kilbride P., Sagona A.P, & Gibson M.I. (2021). Polymer-Mediated Cryopreservation of Bacteriophages. Biomacromolecules, 22(12), 5281-5289.

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Evaluation of Compound Interactions

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All chemicals used in this study were of the highest purity.
Erythromycin, 1,1dimethylbiguanide hydrochloride (metformin), gemfibrozil, p-nitrophenyl phosphate, disodium salt, hexahydrate (pNPP), and calcium chloride dihydrate were purchased from Thermo Scientific. Trimethoprim, diclofenac sodium, gabapentin, and carbamazepine were purchased from Acros Organics. Amoxicillin, albumin from bovine serum (BSA), L-glutathione reduced (GSH), 1-chloro,24-dinitrobenzene (CDNB), potassium chloride, 2-nitrophenyl-β-D-galactopyranoside (ONPG), p-nitrophenyl butyrate (pNPB) were purchased from Sigma Aldrich. L-Leucine-4-nittoanilide, beta-nicotinamide adenine dinucleotide reduced (NADH) were purchased from Alfa Aesar. Sodium pyruvate, magnesium sulfate heptahydrate, and sodium hydrogen carbonate were purchased from Fisher Scientific.

O’Rourke K., Engelmann B., Altenburger R., Rolle-Kampczyk U, & Grintzalis K. (2023). Molecular Responses of Daphnids to Chronic Exposures to Pharmaceuticals. International Journal of Molecular Sciences, 24(4), 4100.

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Synthesis of Polymer Composites

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Aniline monomer (99.0%) was obtained from Research Lab, India. Ammonia solution 25% was received from Chem Solute, Germany. d(+)Glucose anhydrous was purchased from BDH Prolabo Chemicals. Hydrochloric acid (36.0%), sodium hydroxide (98.0%), sodium bicarbonate (99.5%) and boric acid (≥99.5%) were purchased from Sigma-Aldrich, USA. Potassium dichromate (99.0%) and sodium borohydride (99.0%) were obtained from Merck, Germany. Ammonium persulfate (APS) (98.0%), ethanol (HPLC grade), copper sulfate pentahydrate (99.0%) and magnesium sulfate heptahydrate (99.0%) were purchased from Fisher Scientific, UK. 2-Acrylamido-2-methylpropanesulfonic acid (AMPSA) (97.0%), magnesium chloride hexahydrate (98.0%) and calcium nitrate tetrahydrate (98.0%) were obtained from Acros Organics, Germany. Anhydrous lead chloride (99.0%) was received from Oxford Laboratory, India. Sodium chloride (99.0%) was received from Honeywell Company, USA. Aluminum chloride hexahydrate (95.0%) and silver nitrate (99.8%) were purchased from PRS Panreac, Spain. Dodecylbenzene sulfonic acid (DBSA) and potassium sulfate (99%) were purchased from El-Gomhoria Chemical Company, Egypt.

Shokry A., El Tahan A., Ibrahim H., Soliman M, & Ebrahim S. (2019). The development of a ternary nanocomposite for the removal of Cr(vi) ions from aqueous solutions. RSC Advances, 9(67), 39187-39200.

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Purification and Characterization of Protein

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We purchased yeast extract, sodium chloride, LB broth (Miller), potassium phosphate monobasic and dibasic, tris base, tetracycline hydrochloride, magnesium sulfate heptahydrate, imidazole, (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (HEPES), and premade 1 M HEPES buffer (pH 7.3) from Fisher; Triton X-100, tris(2-carboxyethyl)phosphine (TCEP), phenylmethylsulfonyl fluoride (PMSF), bovine serum albumin (BSA), p-nitrophenyl phosphate disodium salt hexahydrate (pNPP), and dimethyl sulfoxide (DMSO) from MilliporeSigma; Phusion and DNase I from New England Biolabs; agar and M9 salts from Becton Dickinson; glucose and Nalpha-4-tosyl-L-arginine methyl ester hydrochloride (TAME) from Acros Organics; tryptone from Research Products International; isopropyl βd-1-thiogalactopyranoside (IPTG) from ChemCruz; kanamycin sulfate from IBI Scientific; carbenicillin from Gemini Bioproducts; lysozyme from Alfa Aesar; 10 kDa spin columns from Sartorius; 4–20% criterion TGX stain-free protein gels from Bio-Rad; and HisTrap and HiTrap columns from GE Healthcare.

Davis B.M., Moyer K.E., Boyd E.F, & Waldor M.K. (2000). CTX Prophages in Classical Biotype Vibrio cholerae: Functional Phage Genes but Dysfunctional Phage Genomes. Journal of Bacteriology, 182(24), 6992-6998.

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Yeast Growth in Furfural and HMF

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Glucose (ACS, cat# D16-3), ammonium chloride (ACS, cat# A661-500), and magnesium sulfate heptahydrate (BioReagents, cat# BP213-1) were from Fisher Scientific (Fair Lawn, NJ, USA). BBL yeast extract (cat# 211929) and DIFCO yeast nitrogen base (cat# 239210) were from Becton, Dickinson and Company (Sparks, MD, USA). Potassium phosphate monobasic (purity >99.0%, cat# RC-084) was from G-Biosciences (St. Louis, MO, USA). Sodium phosphate dibasic (ACS, cat# LC24774-1) was from LabChem Inc (Pittsburgh, PA, USA). Furfural (minimum 98% purity, cat# F0073) was from TCI America (Portland, OR, USA). 5-Hydroxymethyl furfural (HMF) (98% purity, cat# A12475) was from Alfa Aesar (Lancashire LA3 2XY, United Kingdom). Sucrose (ACS, cat#SX1075-3) was from EM Science (Gibbstown, NJ, USA). Vitamin free yeast base (cat#M208-500G) was from HiMedia Labs (India).

Sitepu I., Selby T., Lin T., Zhu S, & Boundy-Mills K. (2014). Carbon source utilization and inhibitor tolerance of 45 oleaginous yeast species. Journal of industrial microbiology & biotechnology, 41(7), 1061-1070.

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