All animal procedures were conducted in accordance with Institutional Animal Care and Use Committee guidelines of Sanford Burnham Prebys Medical Discovery Institute. C57BL/6J 8-week-old male mice were maintained on a diet of 0.2% cuprizone (Bis(cyclohexanone) oxaldihydrazone 98%; Thermo Fisher Scientific, Cat # 370-81-0) for 5-6 weeks. A suspension of ponesimod (30 mg/kg) in vehicle (0.25% methylcellulose (400 cp) with 0.05% polysorbate 80) was prepared weekly after stirring overnight at room temperature and aliquoted accordingly to store at 4°C. Vehicle or ponesimod were delivered orally twice a day (8 hr apart) by re-equilibrating the suspension upon stirring for 30 minutes before the procedure. The ponesimod dose, frequency, delivery, and formulation was based on a prior study published at ACTRIMS.
Cuprizone
Manufactured by Thermo Fisher Scientific
Sourced in United States
Cuprizone is a laboratory chemical compound used in scientific research. It is a copper chelator that has been utilized in various studies, particularly in the field of neuroscience. The core function of Cuprizone is to induce demyelination, a process in which the protective myelin sheath surrounding nerve fibers is damaged or removed. This research tool is commonly employed to create animal models for the study of demyelinating diseases, such as multiple sclerosis.
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Lab products found in correlation
4 protocols using cuprizone
1
Cuprizone-Induced Demyelination and Ponesimod Treatment
2
Fluorescein-labeled Peptide Synthesis
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Peptides were synthesized with an N-terminal fluorescein amine (FAM) tag and provided at >90% purity by (Genscript). Lyophilized aliquots of ~3–4 mg were stored at −80°C and reconstituted with sterile phosphate-buffered saline (PBS) for use in mice. Lysolecithin was from Sigma (Cat# L4129) and cuprizone from Thermo Fisher Scientific (Cat# 370-81-0).
3
Cuprizone-Induced Demyelination and Ponesimod Treatment
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All animal procedures were conducted in accordance with Institutional Animal Care and Use Committee guidelines of the Sanford Burnham Prebys Medical Discovery Institute. C57BL/6J 8‐week‐old male mice were maintained on a diet of 0.2% cuprizone (Bis(cyclohexanone) oxaldihydrazone 98%; Thermo Fisher Scientific, Cat #370‐81‐0) for 5–6 weeks. A suspension of ponesimod (30 mg/kg) in vehicle (0.25% methylcellulose [400 cp] with 0.05% polysorbate 80) was prepared weekly after stirring overnight at room temperature and aliquoted accordingly to store at 4°C. Vehicle or ponesimod were delivered orally twice a day (8 h apart) by re‐equilibrating the suspension upon stirring for 30 min before the procedure. The ponesimod dose, frequency, delivery, and formulation was based on a prior study published at ACTRIMS.
4
Cuprizone and Palmaria Effects on Body Weight
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Cuprizone (Thermo Fisher Scientific-C9012-25G; Waltham, MA, USA) was freshly prepared daily and blended with ground regular chow in 0.2% w/w of CPZ [66 (link)]. Palmaria p. leaf Powder Liquid Extract 2 × 4 oz was purchased from HawaiPharm LLC (Honolulu, HI, USA). The effect of CPZ and Palmaria p. on body weight was measured by the percentage of body weight gain throughout the seven weeks of the experiment following this equation: weight gain (%) = (new weight [W1] − initial weight [W0]/initial weight [W0]) × 100 [67 (link)].
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