Enzyme Linked Immunosorbent Assay method was applied to determine the concentrations of iron and iron-related proteins, including transferrin, ferritin and lactoferrin in CSF and serum. Iron: kit Ab83366 (Abcam Company, Cambridge, UK), transferrin: kit Ab108911 (Abcam Company, Cambridge, UK), ferritin: kit Ab108837 (Abcam Company, Cambridge, UK), lactoferrin: kit E01L0224 (Shanghai Lanji Biological Limited Company, Shanghai, China).
Ab108911
Manufactured by Abcam
Sourced in United Kingdom
Ab108911 is a laboratory equipment product offered by Abcam. It is a device designed for a specific scientific or experimental purpose. The core function of this product is to perform a particular task or operation within a laboratory setting, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Ab108837, by Abcam (2 mentions)
Ab83366, by Abcam (1 mentions)
Procartaplex multiplex immunoassay, by Thermo Fisher Scientific (1 mentions)
Adi eks 800, by Enzo Life Sciences (1 mentions)
Dihm 250, by BioAssay Systems (1 mentions)
Gwb 8da44b, by Aviva Systems Biology (1 mentions)
Gwb 4b6d1a, by Aviva Systems Biology (1 mentions)
2 protocols using ab108911
1
Quantifying Iron-Related Proteins in CSF and Serum
2
Plasma Biomarker Quantification via ELISA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Enzyme-linked immunosorbent assays (ELISA) were conducted according to the manufacturers’ instructions to measure plasma concentrations of haptoglobin (HPT, GWB-8DA44B, Genway Biotech), haemopexin (HPX, GWB-4B6D1A, Genway Biotech), haem oxygenase-1 (HO-1, ADI-EKS-800, Enzo Life Sciences), transferrin (Tf, ab108911, abcam), and ferritin (ab108837, abcam). Colorimetric determination of haem in plasma samples was conducted according to the manufacturer’s instructions (DIHM-250, Bioassay Systems). Samples were diluted prior to testing as follows: 1:50,000 (HPT); 1:1 (haem), 1:40,000 (HPX), 1:10 (Ferritin), 1:6.6 (HO-1), and 1:200 (Tf). Plasma cytokine concentrations were determined by magnetic bead multiplex assay (EPX01A-10228-901 (IFN-γ), EPX01A-10215-901 (IL-10), EPX01A-12001-901 (G-CSF), EPX01A-10213-901 (IL-6), EPX01A-10223-901 (TNFα), EPX01A-10224-901 (IL-1β), EPX01A-10225-901 (IL-4), EPX01A-10204-901 (IL-8), ProcartaPlex Multiplex Immunoassay, Invitrogen, USA) following the manufacturer’s instructions, and analysed on a Luminex 100 (LuminexCorp, Austin, USA) running Bioplex Manager software. Samples were diluted 1:2.5. Samples giving values below the limit of detection were arbitrarily assigned a concentration at the limit of detection for the purposes of statistical analyses (13.008 pg/mL for IFN-γ, 9.5146 pg/mL for IL-10).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!