The co-culture supernatants containing planktonic S. epidermidis were collected in 15 ml centrifuge tubes (Greiner Bio-One International AG, Kremsmünster, Austria) with 1 ml PBS (1X), and centrifuged at 3,345 × g for 10 min at 4°C on day 2 and 7 of co-culture. The number of planktonic bacteria were quantified by serial dilution in 1X PBS and then counting the number of colony forming units on TSB-agar plates (Thermo Fisher Scientific, Inc.) following 24 h incubation at 37°C and 5% CO2.
Tsb agar plates
Manufactured by Thermo Fisher Scientific
TSB-agar plates are a type of microbiological growth medium used for the cultivation and isolation of a wide range of bacteria. The plates are composed of Tryptic Soy Broth (TSB) and agar, providing a nutrient-rich environment for the growth of bacterial colonies.
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Lab products found in correlation
2 protocols using tsb agar plates
1
Quantification of Planktonic S. epidermidis
2
Quantifying S. epidermidis Removal by Ultrasonication
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The test samples were transferred to glass test tubes (Greiner Bio-One International GmbH, Kremsmünster, Austria) containing 1 ml PBS (1X). S. epidermidis were removed by ultrasonic treatment with device settings at 100% for 4 min (BANDELIN BactoSonic, GmbH & Co. KG, Berlin, Germany) at days 2 and 7 of co-culture. The solution in the glass test tube was diluted in 1X PBS and plated onto tryptic soy broth (TSB)-agar plates (Thermo Fisher Scientific, Inc.). Following 24 h incubation at 37°C and 5% CO2, the number of colony forming units were quantified.
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