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Anti human dnmt1 m0231l

Manufactured by New England Biolabs

Anti-human-DNMT1 (M0231L) is a recombinant DNA methyltransferase 1 (DNMT1) protein derived from human cells. DNMT1 is a key enzyme responsible for maintaining DNA methylation patterns during cell division.

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2 protocols using anti human dnmt1 m0231l

1

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The whole cellular lysates were prepared by harvesting the cells in 1 × cell lysis buffer and Western blotting was performed as previously described.6 , 11 (link)–13 , 18 The following antibodies were purchased from Abcam (Cambridge, MA): anti-IL-6 (ab6672, 1:500), and anti-VEGF (ab46154, 1:500). Anti-β-actin (sc-1616, 1:1000) and anti-mouse-DNMT1 (sc-52919, 1:1000) were from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-STAT3 (9139, 1:1000) and anti-phospho-STAT3 (Tyr705, 9131, 1:1000) were from Cell Signaling Technology (Danvers, Massachusetts). Anti-human-FABP4 (AF3150, 1:1000) and anti-mouse-FABP4 (AF1443, 1:500) were from R&D systems (Minneapolis, MN) and the anti-human-DNMT1 (M0231L, 1:500) was from New England Biolabs (Ipswich, MA).
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2

Protein Expression Analysis Workflow

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The whole cellular lysates were prepared by harvesting the cells in 1 × cell lysis buffer and Western blotting was performed as previously described.6 , 11 (link)–13 , 18 The following antibodies were purchased from Abcam (Cambridge, MA): anti-IL-6 (ab6672, 1:500), and anti-VEGF (ab46154, 1:500). Anti-β-actin (sc-1616, 1:1000) and anti-mouse-DNMT1 (sc-52919, 1:1000) were from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-STAT3 (9139, 1:1000) and anti-phospho-STAT3 (Tyr705, 9131, 1:1000) were from Cell Signaling Technology (Danvers, Massachusetts). Anti-human-FABP4 (AF3150, 1:1000) and anti-mouse-FABP4 (AF1443, 1:500) were from R&D systems (Minneapolis, MN) and the anti-human-DNMT1 (M0231L, 1:500) was from New England Biolabs (Ipswich, MA).
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