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Ab115579

Manufactured by Abcam
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Ab115579 is a primary antibody product from Abcam. It is a polyclonal antibody raised in rabbit and targets the protein of interest. The antibody is purified and provided in a liquid format.

Automatically generated - may contain errors

Lab products found in correlation

Anti scl25a13, by Abcam (2 mentions) Dfc420c camera, by Leica (2 mentions) Dm400b microscope, by Leica (2 mentions)

2 protocols using ab115579

1

Immunohistochemistry of Tumor Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Six-micrometre sections were cut from 27 paraffin-embedded tumour samples (Supplementary Table 2) and mounted on Superfrost plus slides. The following antibodies and a standard procedure were used for immunohistochemistry40 (link) with Histogreen as a chromogen: anti-PC (1:100, ab115579, abcam), anti-SCL25A13 (1:50, abcam). Antigen retrieval was performed by boiling slides in Tris-EDTA buffer (pH9) for 45 min for PC and in citrate buffer (pH6) for SLC25A13. Images were acquired with a Leica DM400B microscope with Leica Application Suite software version 2.8.1, and a Leica DFC420C camera (Leica).
Lussey-Lepoutre C., Hollinshead K.E., Ludwig C., Menara M., Morin A., Castro-Vega L.J., Parker S.J., Janin M., Martinelli C., Ottolenghi C., Metallo C., Gimenez-Roqueplo A.P., Favier J, & Tennant D.A. (2015). Loss of succinate dehydrogenase activity results in dependency on pyruvate carboxylation for cellular anabolism. Nature Communications, 6, 8784.
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2

Immunohistochemical Analysis of Tumor Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
6 μm sections were cut from 27 paraffin-embedded tumour samples (Supplementary Table 2) and mounted on Superfrost plus slides. The following antibodies and a standard procedure were used for immunohistochemistry40 (link) with Histogreen as a chromogen: anti-PC (1:100, ab115579, abcam), anti-SCL25A13 (1:50, abcam). Antigen retrieval was performed by boiling slides in Tris-EDTA buffer (pH9) for 45 min for PC and in citrate buffer (pH6) for SLC25A13. Images were acquired with a Leica DM400B microscope with Leica Application Suite software version 2.8.1, and a Leica DFC420C camera.
Lussey-Lepoutre C., Hollinshead K.E., Ludwig C., Menara M., Morin A., Castro-Vega L.J., Parker S.J., Janin M., Martinelli C., Ottolenghi C., Metallo C., Gimenez-Roqueplo A.P., Favier J, & Tennant D.A. (2015). Loss of succinate dehydrogenase activity results in dependency on pyruvate carboxylation for cellular anabolism. Nature Communications, 6, 8784.
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