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Snakeskin dialysis tubing 10 000 mwco

Manufactured by Thermo Fisher Scientific

SnakeSkin dialysis tubing (10,000 MWCO) is a semi-permeable membrane used for the dialysis of macromolecules. It has a molecular weight cutoff (MWCO) of 10,000 daltons, allowing the passage of small molecules while retaining larger molecules.

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2 protocols using snakeskin dialysis tubing 10 000 mwco

1

Iron Oxide Nanoparticle Synthesis and Characterization

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SAHA was purchased from LC Laboratories (Woburn, MA). LAQ was purchased from Selleck Chemicals (Houston, TX). Ferric chloride hexahydrate, cholesterol, 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p′-disulfonic acid sodium salt (ferrozine), sodium ascorbate, resazurin sodium salt were purchased from Sigma-Aldrich (St. Louis, MO). Dipalmitoylphophatidylcholine (DPPC) and dipalmitoylphosphatidylethanolamine-N-[methoxy (polyethylene glycol)-2000] (DPPE-PEG2000) were purchased from NOF America Corporation (White Plains, NY). Radioactive iron-59 (Fe-59) was purchased from PerkinElmer (Waltham, MA) in the form of FeCl3, with specific activity > 5 Ci/g. SnakeSkin dialysis tubing (10,000 MWCO), Dulbecco’s modified eagle medium (DMEM), 0.05% trypsin/EDTA solution, heat-inactivated fetal bovine serum (FBS), penicillin-streptomycin solution (100×), L-glutamine solution (100×) and non-essential amino acids solution (100X) were purchased from ThermoFisher Scientific (Waltham, MA). The fluorophore 2′,7′-dichlorodi-hydrofluorescein diacetate (H2DCFDA) was purchased from Life Technologies (Grand Island, NY). Annexin V-FITC apoptosis detection kit was purchased from BD Biosciences (San Jose, CA).
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2

Synthesis and Characterization of Polymeric Nanoparticles

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NHS-PEG-b-PLA copolymers and Mal-PEG-b-PLA copolymers with a molecular weight of 6000 Da were purchased from Nanosoft Polymers. DiI was purchased from Biotium. Ham’s F10 medium and bovine serum albumin (BSA) were obtained from Gibco and GenDEPOT, respectively. Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and antibiotic-antimycotics were purchased from Biowest. The DNA Mini Kit and RNeasy Mini Kit were purchased from QIAGEN. TOPscript™ complementary DNA (cDNA) Synthesis Kit and qPCR SyGreen Mix were purchased from Enzynomics and PCR BIOSYSTEMS, respectively. SnakeSkin™ Dialysis Tubing (10,000 MWCO) was purchased from Thermo and pluriStrainer® 500 µm was purchased from pluriSelect. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assay kit was obtained from iNtRON Biotechnology. Hank’s balanced salt solution (HBSS), dimethyl sulfoxide (DMSO), nicotinamide, D-glucose, L-glutamine, calcium chloride dihydrate, isobutylmethylxanthine, ciprofloxacin, N-(2-Hydroxyethyl) piperazine-N′-(2-ethanesulfonic acid) (HEPES), sodium bicarbonate, collagenase type V, acridine orange (AO), propidium iodide (PI), and 3,3',5-triiodo-L-thyronine sodium salt (T3) were purchased from Sigma Aldrich. Human/Canine/Porcine Insulin Quantikine ELISA Kit purchased from R&D Systems. All other chemicals and reagents were of analytical grade.
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