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Cyclohexanone chx

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Cyclohexanone (CHX) is a colorless, oily liquid that is commonly used as a solvent and an intermediate in the production of various chemicals and pharmaceuticals. It has a distinctive odor and is miscible with most organic solvents. Cyclohexanone is a key component in the synthesis of nylon and other important industrial chemicals.

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2 protocols using cyclohexanone chx

1

Immunoblot Analysis of NF-κB Pathway

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Phospho-IKKα/β (Ser176/180), I-κBα, phosphor-I-κBα (Ser32) and anti-α-tubulin (3873p) were purchased from Cell Signaling Technology (Beverly, MA). Anti-TRAF6 (H-274), anti-p65 (C-20), anti-ub (P4D1) and Sp1 (sc-59-G) were from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-CHIP antibody was developed in our lab (21 (link)). MG132 was purchased from Millipore (Billerica, MA). Cyclohexanone (CHX) was purchased from Amresco (AMRESCO Inc., OH).
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2

Regulation of NF-κB Signaling by CHIP

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pCMV/Myc-TRAF6, TRAF2, and TRAF5 was provided by Dr. Lingqiang Zhang (State Key Laboratory of Proteomics, Beijing, China). pEFNeo/HA-CHIP, pRKIM/Myc-CHIP, and pRKIM/Myc-CHIP (H260Q) were generated in our lab as described previously.23 (link) I-κBα, phosphor-I-κBα (Ser32) and anti-α-tubulin (3873p) were purchased from Cell Signaling Technology (Beverly, MA). Anti-TRAF6 (H-274) and anti-p65 (C-20) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-CHIP antibody was developed in our lab.16 (link) MG132 was purchased from Millipore (Billerica, MA). Cyclohexanone (CHX) was purchased from Amresco (AMRESCO Inc., OH).
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