Embed812

Manufactured by Thermo Fisher Scientific

Sourced in United States

Embed812 is a laboratory embedding medium used for the preparation of specimens for electron microscopy. It is a low-viscosity, epoxy-based resin that provides a firm, durable support for sectioning and observation of samples under an electron microscope.

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Lab products found in correlation

Glutaraldehyde, by Ted Pella (2 mentions) Lkb 3 ultratome, by Leica (2 mentions) Graded ethanol and propylene oxide, by Thermo Fisher Scientific (2 mentions) Jem 2010, by JEOL (2 mentions) Uranyl acetate, by Ted Pella (1 mentions) Lead citrate, by Ted Pella (1 mentions) H 7600 electron microscope, by Hitachi (1 mentions)

2 protocols using embed812

Transmission Electron Microscopy Tissue Analysis

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TEM samples were analyzed by a transmission electron microscope (JEM-2010, JEOL) installed in the Center for University-Wide Research Facilities (CURF) at Chonbuk National University. After fixation of arterial samples in 2.5% glutaraldehyde (TED PELLA, USA) in PBS (pH, 7.2), specimens were post-fixed in 1% osmium tetroxide (Heraeus, South Africa), dehydrated in graded ethanol and propylene oxide (Acros Organics, USA), and then embedded in Epoxy resin (Embed812, NMA; nadic methyl anhydride. DDSA; dodenyl succinic anhydride. DMP-30, USA) as used previously. Serial ultrathin sections were cut on an LKB-III ultratome (LEICA, Germany). Ultrathin sections were stained with uranyl acetate (TED PELLA, USA) and lead citrate (TED PELLA, USA) and examined with the aid of a Hitachi H7600 electron microscope (Hitachi, Japan) at an accelerating voltage of 100 kV.

Jeong J.K., Lee Y.J., Jeong S.Y., Jeong S., Lee G.W, & Park S.Y. (2017). Autophagic flux induced by graphene oxide has a neuroprotective effect against human prion protein fragments. International Journal of Nanomedicine, 12, 8143-8158.

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Transmission Electron Microscopy of SK-N-SH Cells

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TEM samples were analyzed by Transmission Electron Microscope (JEM-2010, JEOL) installed in the Center for University-Wide Research Facilities (CURF) at Chonbuk National University. After fixing SK-N-SH cell samples in 2.5% glutaraldehyde (TED PELLA, USA) in PBS (pH: 7.2), specimens were post-fixed in 1% osmium tetroxide (Heraeus, South Africa), dehydrated in graded ethanol and propylene oxide (Acros Organics, USA), and then embedded in epoxy resin (Embed812, NMA: Nadic methyl anhydride, DDSA: Dodenyl Succinic Anhidride, DMP-30, USA), as used previously. Serial ultrathin sections were cut on an LKB-III ultratome (LEICA, Germany). Ultrathin sections were stained with uranyl acetate (TED PELLA, USA) and lead citrate (TED PELLA, USA) and were examined using a Hitachi H7600 electron microscope (Hitachi, Japan) at an accelerating voltage of 100 kV.

Moon J.H., Lee J.H., Lee Y.J, & Park S.Y. (2016). Hinokitiol protects primary neuron cells against prion peptide-induced toxicity via autophagy flux regulated by hypoxia inducing factor-1. Oncotarget, 7(21), 29944-29957.

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