The effect of acetaminophen metabolites on methemoglobinemia was tested in vitro by adding them to a sample of perfusate (1:1 whole blood with modified Krebs-Henseleit). P-aminophenol (Sigma Aldrich Canada, Oakville, ON, Canada) was added in a concentration of 100 µg/mL and let sit at room temperature. Methemoglobin was measured, as described above, at 30 and 90 min. N-acetyl-p-benzoquinone imine (NAPQI) (Sigma Aldrich Canada, Oakville, ON, Canada) was added to a separate sample of perfusate at a concentration of 125 µg/mL and methemoglobin was again measured after 90 and 180 min at room temperature. For comparison, acetaminophen (Sigma Aldrich Canada, Oakville, ON, Canada) was added to perfusate at a concentration of 10 mg/mL and methemoglobin was measured after 60 min. The effect of CCl4 on hemolysis was tested in vitro by adding CCl4 (Sigma Aldrich Canada, Oakville, ON, Canada) to whole blood in a concentration of 10 µL/mL and measuring plasma free hemoglobin after 30 min at room temperature, as detailed above. Free hemoglobin was measured in porcine whole blood after 30 min at room temperature without the addition of CCl4 for control comparison.
P aminophenol
Manufactured by Merck Group
Sourced in United States
P-aminophenol is a chemical compound used in the manufacture of various pharmaceutical and industrial products. It serves as a precursor in the synthesis of various organic compounds. The core function of P-aminophenol is to provide a starting material for the production of other chemicals and materials.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Merck Group (3 mentions)
Tetrahydrofuran, by Merck Group (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
P benzoquinone, by Merck Group (1 mentions)
2 ethoxyethanol, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Vanillin, by Merck Group (1 mentions)
Araldite mt 35700, by Huntsman (1 mentions)
Acetaminophen, by Merck Group (1 mentions)
Catechol, by Merck Group (1 mentions)
Sulfanilic acid, by Merck Group (1 mentions)
Sulfuric acid, by Thermo Fisher Scientific (1 mentions)
Potassium ferrocyanide, by HiMedia (1 mentions)
Potassium hydrogen phosphate, by HiMedia (1 mentions)
Dopamine, by Merck Group (1 mentions)
P phenylenediamine, by Merck Group (1 mentions)
Labostar, by Siemens (1 mentions)
P aminobenzoic acid, by Merck Group (1 mentions)
α al2o3, by Merck Group (1 mentions)
Graphite powder, by Merck Group (1 mentions)
8 protocols using p aminophenol
1
Acetaminophen Metabolites and Methemoglobinemia
2
Synthesis and Characterization of Benzoxazine
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Bisphenol-F and aniline-based benzoxazine Araldite MT35700® (BF-a) was obtained from Huntsman Corporation (Basel, Switzerland), and 4,4′-methylenedianiline (MDA) from Fluka (Buchs, Switzerland). p-Aminophenol, p-benzoquinone, ethanol, 2-ethoxyethanol, methanol, tetrahydrofuran and vanillin were supplied from Sigma Aldrich (Taufkirchen, Germany). 4-hydroxybenzaldehyde was from ABCR (Karlsruhe, Germany), and DOPO was from TCI (Zwijndrecht, Belgium). All chemicals were used without further purification.
3
Analytical Reagents for Pharmaceuticals
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Acetaminophen (APAP), as well as catechol (CAT), hydroquinone (HQ), and p-aminophenol (APA), were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other reagents were of analytical reagent grade and were obtained from Riedel-de Haën (Seelze, Germany). Water purified with a Mili-Q system was used in all the experiments.
4
Electrochemical Sensing Platform Fabrication
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Graphite powder (≤20 µM), α-Al2O3, potassium permanganate, sodium nitrate, hydrogen peroxide (H2O2), LiClO4, potassium chloride, sodium hydroxide, ruthenium hexamine chloride, dopamine, p-aminothiophenol, p-aminophenol, p-phenylenediamine, p-aminobenzoic acid and sulfanilic acid were purchased from Sigma-Aldrich. Sulfuric acid was purchased from Fisher-Scientific. Potassium ferrocyanide and potassium hydrogen phosphate were purchased from Hi-Media. Acetonitrile was purchased from SRL. All the chemicals were used as received without any purification. Ultrapure water from Siemens LaboStar (resistivity ~18.2 MΩ.cm) system was used to prepare phosphate buffer and other aqueous solutions.
5
Daunorubicin Extraction Protocol
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Ethylene glycol (EG), (1S)-(+)-camphor-10-sulfonic acid (CSA), p-aminophenol, salicylic acid (SA), methyltrioctylammoniumchloride (MTOAC), n-butanoic acid (BA), 1-undecanol, l-menthol, 1-octanol, thymol and pure standard of daunorubicin hydrochloride (purity higher than 95 %) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetone, acetonitrile (for spectroscopy), methanol (HPLC grade), sodium dodecyl sulfate (SDS), decanoic acid, 1-decyl-3-methylimidazolium chloride ([DMIM]Cl), Tetrabutyl ammonium chloride (TBAC), Na2HPO4, NaCl, KOH and HCl (37 %) were purchased from Merck (Darmstadt, Germany). A stock solution of DNR at 1000 mg L−1 was prepared in 1 % (v/v) formic acid in water:methanol (50:50 % v/v). Working solutions were prepared by daily dilution of the stock solution.
6
Adipocyte Differentiation Assay Protocol
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3-Isobutyl-1-methylxanthine (IBMX), dexamethasone (DEX), insulin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT), Oil Red O, dimethyl sulfoxide (DMSO), m-hydroxyphenylglycine, p-aminophenol, ellagic acid, and 4-hydroxycoumarin were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Erycibelline was purchased from ChemFaces (Wuhan, China). 5-Hydroxy-2-pyridinemethanol was purchased from IS Chemical Technology (Shanghai, China). Dulbecco's Modified Eagle's Medium (DMEM), bovine serum (BS), and fetal bovine serum (FBS) were purchased from Invitrogen Inc. (Grand Island, NY, USA). All other chemicals were analytical reagent grade.
7
Fabrication of Electrochemical Sensors
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All chemicals used in fabrication
including SU8 2002 and KMPR 1010 photoresists, SU8 developer, and
Omnicoat adhesion promoter were purchased from KayukaAM (Westborough,
Massachusetts). Hydroxymethylferrocene (97%), p-aminophenol
(98%), (±)-epinephrine hydrochloride (98%), and monobasic potassium
phosphate (99%) were purchased from Sigma-Aldrich (St. Louis, Missouri).
85 w/w% phosphoric acid (ACS grade) was purchased from Fischer Chemical
(Hampton, New Hampshire). Potassium nitrate (reagent grade) was purchased
from VWR Life Sciences (Radnor, Pennsylvania). A 1,4-dimethyoxybenzene
derivative (C7) was synthesized40 (link) and provided
by the Zhang group from Argonne National Laboratory. A tri(dialkylamino)cyclopropenium
derivative (HS-cyclopropenium) was synthesized41 (link) and provided by the Sanford group from the University of
Michigan, Ann Arbor. Eighteen MΩ DI water was obtained from
a Milli-Q purification system. All other chemicals were used as purchased
without further purification. Glass slides (2” × 3”)
were purchased from Corning Inc. (Corning, New York). Anisotropically
conductive film for making device connections was purchased from 3
M (St. Paul, Minnesota). Photomasks for the device were purchased
from Front Range Photomasks (Phoenix, Arizona).
including SU8 2002 and KMPR 1010 photoresists, SU8 developer, and
Omnicoat adhesion promoter were purchased from KayukaAM (Westborough,
Massachusetts). Hydroxymethylferrocene (97%), p-aminophenol
(98%), (±)-epinephrine hydrochloride (98%), and monobasic potassium
phosphate (99%) were purchased from Sigma-Aldrich (St. Louis, Missouri).
85 w/w% phosphoric acid (ACS grade) was purchased from Fischer Chemical
(Hampton, New Hampshire). Potassium nitrate (reagent grade) was purchased
from VWR Life Sciences (Radnor, Pennsylvania). A 1,4-dimethyoxybenzene
derivative (C7) was synthesized40 (link) and provided
by the Zhang group from Argonne National Laboratory. A tri(dialkylamino)cyclopropenium
derivative (HS-cyclopropenium) was synthesized41 (link) and provided by the Sanford group from the University of
Michigan, Ann Arbor. Eighteen MΩ DI water was obtained from
a Milli-Q purification system. All other chemicals were used as purchased
without further purification. Glass slides (2” × 3”)
were purchased from Corning Inc. (Corning, New York). Anisotropically
conductive film for making device connections was purchased from 3
M (St. Paul, Minnesota). Photomasks for the device were purchased
from Front Range Photomasks (Phoenix, Arizona).
8
Adsorption of Paracetamol onto Modified Clay
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All chemicals were of analytical grade. The clay used was Wyoming Na-montmorillonite SWY-2 obtained from the Source Clays Registry (Clay Mineral Society, Colombia, MO). Quartz sand (grain size 0.8-1.2 mm) was obtained from Negev industrial minerals (Israel). Octadecyltrimethylammonium (ODTMA) bromide was obtained from Sigma Aldrich. Paracetamol was obtained from Birzeit pharmaceutical company (Ramallah-Palestine). Activated Charcoal (12-20 mesh) was obtained from Sigma (Sigma Chemical Company, USA). De-ionized water was used to prepare all solutions. Methanol, acetonitrile, tetrahydrofuran, and water were both HPLC grade and purchased from Sigma Aldrich. Magnesium sulfate, hydrogen peroxide, and p-aminophenol were purchased from Sigma Aldrich. High purity diethyl ether (>99%) was purchased from Biolab (Israel); orthophosphoric acid was obtained from Riedel-De Haën (Germany). Microbiological growth media (MacConkey agar, Blood Agar, Eosin Methylene Blue (EMB) Agar, and Mannitol Salt Agar (MSA)) were obtained from Sigma-Aldrich company; API-20E test Kit was obtained from Biomerieux.
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