Dspe peg2000 mal

Distearoyl-glycerophosphoethanolamine-polyethyleneglycol₂₀₀₀-maleimide (DSPE-PEG₂₀₀₀-Mal) was purchased from NOF Corporation (Tokyo, Japan). Peptide (CYKLEGTTRLTRKRGLKLA) was custom synthesized by GL Biochem (Shanghai, China). Paclitaxel (PTX) was purchased from Norzer Pharmaceutical Co., Ltd. Alpha linolenic acid (ALA) was purchased from ANPEL Laboratory Technologies (Shanghai, China). Soybean phosphatidylcholine (SPC), unesterified cholesterol (UC), triolein (TO) and cholesteryl oleate (CO) were from Lipoid (Ludwigshafen, Germany), Wako Ltd. (Tokyo, Japan), Sinopharm Chemical Reagent Co., Ltd (Beijing, China) and InnoChem Science & Technology Co., Ltd. (Beijing, China), respectively. Solutol^® HS 15 was produced by BASF (Germany). Dicyclohexylcarbodiimide (DCC) and 4-dimethylaminopyridine (DMAP) were obtained from Sinopharm Group Co., Ltd. Cell Counting Kit-8 (CCK- 8) was obtained from SolarbioScience & Technology Co., Ltd (Beijing, China). Coumarin-6 (COU) was purchased from Sigma-Aldrich Company (USA). TUNEL Kit was obtained from KeyGEN Biotech Co., Ltd. (Nanjing, China). Enzyme-linked Immunosorbent Assay (ELISA) Kit for LDLR was obtained from USCN Life Science Inc. Modified eagle medium (MEM), DMEM, penicillin-streptomycin, trypsin and Hoechst 33258 were obtained from Macgene Technology (Beijing, China).

Lipids, such as 1,2-distearoyl-sn-glycero-3-phosphatidylcholine (DSPC), N-(carbonyl-methoxypolyethyleneglycol2000)-1,2-distearoyl-sn-glycero-3 phosphatidyl-ethanolamine (DSPE-PEG2000), and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-2000] (ammonium salt) (DSPE-PEG2000-Mal), were purchased from NOF Corporation (Tokyo, Japan). Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) and L-cysteine were purchased from FUJIFILM Wako Pure Chemical Corporation (Tokyo, Japan). Herceptin^® (trastuzumab) was purchased from Chugai Pharmaceutical Co., Ltd. (Tokyo, Japan). 1,1-dioctadecyl-3,3,3,3-tetramethyl-indocarbocyanine perchlorate (DiI) was purchased from Setareh Biotech LLC (Eugene, OR, USA).