Lux cellulose 2 column

Racemic PCB149 (99.9%) containing 50% of (-)-PCB149 and 50% of (+)-PCB149 was provided by Dr. Ehrenstorfer GmbH (Germany). The racemate was separated and prepared on a Lux Cellulose-2 column (250 × 4.6 mm, 5 μm, Phenomenex, Torrance, CA) using an Agilent 1200 series high performance liquid chromatography (HPLC) instrument (Wilmington, DE). The mobile phase was 100% n-hexane at a flow rate of 1.0 mL/ min. The atropisomers (-)-PCB149 and (+)-PCB149[26 (link)] were repeatedly collected separately and concentrated to dryness using a nitrogen-evaporator (Hangzhou Allsheng Instruments company, China) and then dissolved in acetone (Fisher Scientific, USA). The purities and concentrations of the isomers were determined using a gas chromatography-mass spectrometry (GC-MS). Both of the (-) and (+) PCB149 atropisomers were pure (>98.0%).
The reconstituted water also considered as standard solution for embryo-larvae was prepared in the lab with the formula of iso-7346-3, which contained 2 mM Ca²⁺, 0.5 mM Mg²⁺, 0.75 mM Na⁺ and 0.074 mM K⁺ (ISO, 1996) were used for following tests. All organic reagents in this study were of HPLC-grade and the other reagents of analytical grade.

Racemic PCB149 (99.9%) was provided by Dr. Ehrenstorfer GmbH (Germany). The racemate was separated and prepared on a Lux Cellulose-2 column (250 × 4.6 mm, 5 μm, Phenomenex, Torrance, CA) using an Agilent 1200 series high performance liquid chromatography (HPLC) instrument (Wilmington, DE) with 100% n-hexane as the mobile phase at a flow rate of 1.0 mL/min. The enantiomers (−)-PCB149 and (+)-PCB14948 (link) were repeatedly collected separately, concentrated to dryness using a nitrogen-evaporator (Hangzhou Allsheng Instruments company, China) and then dissolved in acetone (Fisher). The purities and concentrations of the isomers were determined by gas chromatography-mass spectrometry (GC-MS).
An Agilent 7890A/5975C GC-MS system equipped with a Chirasil-Dex capillary column (25 m × 0.25 mm; I.D. 0.25 μm df) from Agilent was used for purity and concentration determinations, and the oven temperature was programmed as follows: 60 °C for 2 min, 60–150 °C at 10 °C·min⁻¹ (held for 5 min), 150–180 °C at 1 °C·min⁻¹ (held for 22 min). The SIM ions were m/z 360 (quantification ion), 362, and 3587 (link). The purities of (−)-PCB149 and (+)-PCB149 were higher than >98.0%.