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Nadph standard

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NADPH standard is a chemical compound used as a reference material in various biochemical and analytical applications. It serves as a standard for the accurate quantification of NADPH (Nicotinamide Adenine Dinucleotide Phosphate), an important cofactor involved in numerous cellular processes.

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Nadp nadph glo assay kit, by Promega (1 mentions) Nadp nadph glo assays kit, by Promega (1 mentions)

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NADPH

2 protocols using nadph standard

1

Quantifying Cellular NADP/NADPH Levels

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The total cellular NADP+ and NADPH levels were determined by an NADP/NADPH quantitation colorimetric assay kit with an NADPH standard (BioVision Inc., San Francisco, CA) and normalized by the total protein of each sample. To determine the NADPH/NADP+ ratio, an NADP/NADPH-Glo assay kit (Promega, Madison, WI) was also used, following the manufacturers’ protocol. The relative NADPH/NADP+ ratio was normalized by that of control cells (or treatment control) under matrix-attached condition.
Zhang Y., Xu Y., Lu W., Li J., Yu S., Brown E.J., Stanger B.Z., Rabinowitz J.D, & Yang X. (2022). G6PD-mediated increase in de novo NADP+ biosynthesis promotes antioxidant defense and tumor metastasis. Science Advances, 8(29), eabo0404.
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2

Quantifying NADP+, NADPH, and ATP

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NADP+ and NADPH were also quantified using the NADP/NAPDH quantitation colorimetric assays with a NADPH standard (BioVision, Inc, San Francisco, CA) and normalized by protein concentration. The NADP/NADPH ratio was measured by a NADP/NADPH-Glo™ assays kit (Promega, Madison, WI), following the manufacturers’ protocol, and the relative NADPH/NADP+ ratio is normalized by that of control cells (or treatment control) under matrix-attached condition. ATP levels were detected by an ATP colorimetric assays kit (Abcam) and normalized by protein concentration, and the relative ATP levels is normalized by that of control cells (or treatment control) under matrix-attached condition.
Zhang Y., Xu Y., Lu W., Ghergurovich J.M., Guo L., Blair I.A., Rabinowitz J.D, & Yang X. (2020). Upregulation of antioxidant capacity and nucleotide precursor availability suffices for oncogenic transformation. Cell metabolism, 33(1), 94-109.e8.
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