Anti lineage cocktail 1

Cells were surface-stained with monoclonal antibodies in PBS containing 10% (v/v) human serum, 2.5% (v/v) FBS and 0.1% (w/v) azide on ice. The following antibodies were used: anti-lineage cocktail 1, anti-CD123 (7G3), anti-HLA-DR (L243), anti-CD40 (5C3), anti-CD11c (B-ly6), anti-CD86 (2331, FUN-1), anti-CD19 (4G7), anti-CD69 (FN50), all from BD Biosciences and anti-CD14 (61D3), anti-CD169 (7–239), anti-CD3 (OKT-3), anti-CD56 (MEM188), anti-CD80 (2D10), all from eBioscience.
All flow cytometric parameters of cells were acquired on a FACSCalibur (BD Biosciences). Frequencies were related to the indicated parent populations; geometric means of fluorescent cells were indicated as mean fluorescence intensity (MFI). Data were analyzed with the FlowJo software.

Cells were surface‐stained with monoclonal antibodies in PBS containing 10% (v/v) human serum, 2.5% (v/v) FCS, and 0.1% (w/v) azide on ice. The following antibodies were used: anti‐lineage cocktail 1, αCD123 (7G3), αHLA‐DR (L243), αCD40 (5C3), αCD11c (B‐ly6), αCD86 (2331 FUN‐1), αCD19 (4G7), αCD69 (FN50), all from BD Biosciences (Heidelberg, Germany) and αCD14 (61D3), αCD169 (7–239), αCD3 (OKT‐3), αCD56 (MEM188), αCD80 (2D10), all from eBioscience.
All flow cytometric parameters of cells were acquired on a FACSCalibur (BD Biosciences). Frequencies were related to the indicated parent populations; geometric means of fluorescent cells were indicated as mean fluorescent intensity (MFI). Data were analyzed with the FlowJo software (Tree Star, Ashland, OR).